Clostridium phage phiCD211

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 190 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A8WIV9|A0A0A8WIV9_9CAUD Transcriptional regulator Putative repressor RepR OS=Clostridium phage phiCD211 OX=1582152 GN=PHICD211_20168 PE=4 SV=1
MM1 pKa = 7.35LNIYY5 pKa = 8.13ITNLGKK11 pKa = 10.76YY12 pKa = 9.92NEE14 pKa = 4.68GEE16 pKa = 4.65LIGEE20 pKa = 4.18WAEE23 pKa = 3.9LPVNEE28 pKa = 5.18EE29 pKa = 4.0EE30 pKa = 4.43LQEE33 pKa = 4.02ILDD36 pKa = 4.09RR37 pKa = 11.84IGINEE42 pKa = 4.11EE43 pKa = 3.86YY44 pKa = 10.37EE45 pKa = 4.19EE46 pKa = 4.23YY47 pKa = 10.55FITDD51 pKa = 4.19FEE53 pKa = 4.46TDD55 pKa = 3.14IEE57 pKa = 4.13GLEE60 pKa = 4.06ISEE63 pKa = 4.61HH64 pKa = 6.88SNIEE68 pKa = 3.93QLNEE72 pKa = 3.5LAMKK76 pKa = 10.49LEE78 pKa = 4.04RR79 pKa = 11.84LEE81 pKa = 4.09EE82 pKa = 4.11YY83 pKa = 10.38EE84 pKa = 4.3IEE86 pKa = 4.07EE87 pKa = 4.21LKK89 pKa = 11.11ALLEE93 pKa = 3.99RR94 pKa = 11.84GYY96 pKa = 10.46IDD98 pKa = 4.53FYY100 pKa = 11.98DD101 pKa = 3.7MLEE104 pKa = 3.94GDD106 pKa = 3.8ICRR109 pKa = 11.84LSGNYY114 pKa = 8.55TFIQLDD120 pKa = 3.78DD121 pKa = 4.56RR122 pKa = 11.84SPLDD126 pKa = 3.95DD127 pKa = 4.2EE128 pKa = 4.56NLGRR132 pKa = 11.84SYY134 pKa = 10.71IEE136 pKa = 4.67EE137 pKa = 4.19ICCGDD142 pKa = 4.03LSCIEE147 pKa = 4.32NIAYY151 pKa = 10.36YY152 pKa = 10.5FDD154 pKa = 4.18IEE156 pKa = 4.37AFSRR160 pKa = 11.84DD161 pKa = 3.43LRR163 pKa = 11.84FDD165 pKa = 3.49RR166 pKa = 11.84DD167 pKa = 3.77MIIEE171 pKa = 4.06NLYY174 pKa = 10.89EE175 pKa = 4.01EE176 pKa = 5.48DD177 pKa = 3.09IEE179 pKa = 4.74YY180 pKa = 10.07YY181 pKa = 11.07EE182 pKa = 4.39NMTDD186 pKa = 4.43IEE188 pKa = 4.11FAEE191 pKa = 4.33NYY193 pKa = 9.54IEE195 pKa = 4.11RR196 pKa = 11.84LGDD199 pKa = 3.48IEE201 pKa = 4.93EE202 pKa = 4.88LGQEE206 pKa = 3.9TLEE209 pKa = 4.28RR210 pKa = 11.84YY211 pKa = 9.23FNYY214 pKa = 10.33EE215 pKa = 3.87LYY217 pKa = 9.48GTDD220 pKa = 3.8LRR222 pKa = 11.84YY223 pKa = 10.57EE224 pKa = 4.36NICIASNYY232 pKa = 9.13LAIVSS237 pKa = 3.87

Molecular weight: 28.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A8WEC6|A0A0A8WEC6_9CAUD Structural protein OS=Clostridium phage phiCD211 OX=1582152 GN=PHICD211_20006 PE=4 SV=1
MM1 pKa = 7.43AQKK4 pKa = 10.45CDD6 pKa = 3.29QKK8 pKa = 11.21AKK10 pKa = 9.81KK11 pKa = 9.43RR12 pKa = 11.84HH13 pKa = 5.59RR14 pKa = 11.84NVTKK18 pKa = 10.61KK19 pKa = 10.62INLKK23 pKa = 10.17SYY25 pKa = 10.41NIEE28 pKa = 3.67KK29 pKa = 10.33EE30 pKa = 3.82KK31 pKa = 11.19YY32 pKa = 7.72EE33 pKa = 4.09RR34 pKa = 11.84EE35 pKa = 3.95KK36 pKa = 11.26NRR38 pKa = 11.84INIFRR43 pKa = 11.84RR44 pKa = 11.84FRR46 pKa = 4.28

Molecular weight: 5.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

190

0

190

37964

34

2176

199.8

23.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.667 ± 0.246

1.509 ± 0.135

6.554 ± 0.159

8.479 ± 0.304

3.88 ± 0.135

4.72 ± 0.249

1.225 ± 0.09

9.78 ± 0.231

11.037 ± 0.321

8.822 ± 0.166

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.244 ± 0.116

7.968 ± 0.25

1.862 ± 0.105

2.726 ± 0.127

3.553 ± 0.134

6.319 ± 0.188

4.939 ± 0.212

4.849 ± 0.151

0.753 ± 0.07

5.113 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski