Magnetospira sp. (strain QH-2) (Marine magnetic spirillum (strain QH-2))

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Thalassospiraceae; Magnetospira; unclassified Magnetospira

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3700 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W6KPV5|W6KPV5_MAGSQ 2-oxo-carboxylic acid reductase (Glyoxalate reductase) (2-ketoaldonate reductase) OS=Magnetospira sp. (strain QH-2) OX=1288970 GN=MGMAQ_3928 PE=3 SV=1
MM1 pKa = 7.48KK2 pKa = 10.29KK3 pKa = 10.36VLLTTTALVVAGAFASQAQAADD25 pKa = 4.41PIKK28 pKa = 10.74LSVGGHH34 pKa = 4.37MNQWIMFADD43 pKa = 3.78SDD45 pKa = 4.67KK46 pKa = 10.65ATQTGGVDD54 pKa = 3.39TLSDD58 pKa = 3.56TEE60 pKa = 4.9VYY62 pKa = 10.61FKK64 pKa = 11.45GDD66 pKa = 3.27TTLEE70 pKa = 4.18NGLKK74 pKa = 10.0VSVVIQLEE82 pKa = 4.39GEE84 pKa = 3.98EE85 pKa = 4.12DD86 pKa = 3.64TATNNADD93 pKa = 3.32EE94 pKa = 4.46QYY96 pKa = 11.53VVISSDD102 pKa = 3.18SLGTLRR108 pKa = 11.84VGDD111 pKa = 4.59KK112 pKa = 8.58EE113 pKa = 4.3TALATIKK120 pKa = 10.29IGSPAVGYY128 pKa = 9.93GGPTDD133 pKa = 4.4ADD135 pKa = 3.63DD136 pKa = 4.1VVAPAFAGASDD147 pKa = 3.48QFANAEE153 pKa = 4.14FVDD156 pKa = 4.98DD157 pKa = 5.56DD158 pKa = 4.9DD159 pKa = 4.96NQVTYY164 pKa = 10.51LSPSFAGFQVGGSWVPHH181 pKa = 6.07FAAGDD186 pKa = 3.79AQGGPNTNPVGGNNDD201 pKa = 3.66GGWGVGAIYY210 pKa = 10.55SADD213 pKa = 3.44YY214 pKa = 11.01DD215 pKa = 4.32GIGVGVSGGYY225 pKa = 10.13AYY227 pKa = 10.49HH228 pKa = 7.85DD229 pKa = 4.31AGADD233 pKa = 2.92IWQAGAKK240 pKa = 9.99VSFAGFTVSGGWQRR254 pKa = 11.84TDD256 pKa = 2.85VHH258 pKa = 6.48EE259 pKa = 4.39VQLGNTNNALSVDD272 pKa = 3.89GVEE275 pKa = 4.41GTGWEE280 pKa = 4.2AGVSYY285 pKa = 9.24ATGPYY290 pKa = 9.89AVSLVYY296 pKa = 10.32GAGEE300 pKa = 5.01DD301 pKa = 3.67EE302 pKa = 4.59DD303 pKa = 5.18TYY305 pKa = 11.98ADD307 pKa = 3.63TDD309 pKa = 3.77KK310 pKa = 11.83DD311 pKa = 3.93EE312 pKa = 4.86LKK314 pKa = 10.67VWAVGANYY322 pKa = 11.0SMGAGVMMKK331 pKa = 9.35GTIYY335 pKa = 10.38HH336 pKa = 6.99ADD338 pKa = 3.55YY339 pKa = 11.17NGEE342 pKa = 4.21TNTTGVGGDD351 pKa = 3.72TASGWGGIVGMAISFF366 pKa = 3.8

Molecular weight: 37.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W6K585|W6K585_MAGSQ Uncharacterized protein OS=Magnetospira sp. (strain QH-2) OX=1288970 GN=MGMAQ_0348 PE=4 SV=1
MM1 pKa = 8.11RR2 pKa = 11.84IPGTRR7 pKa = 11.84LSLLLVALPSALLAPLLGLALLALRR32 pKa = 11.84RR33 pKa = 11.84GSALPISLLALRR45 pKa = 11.84RR46 pKa = 11.84WATLPTALLATLLGTALLPTGGRR69 pKa = 11.84TTLPATLLAASPRR82 pKa = 11.84AALPLSLRR90 pKa = 11.84ALGRR94 pKa = 11.84RR95 pKa = 11.84PTLPVALLALRR106 pKa = 11.84WRR108 pKa = 11.84TPLPVSRR115 pKa = 11.84LTALPGTALPLSLLSSRR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 11.84PALPVALLALRR145 pKa = 11.84WRR147 pKa = 11.84PPLPLPWRR155 pKa = 11.84RR156 pKa = 11.84RR157 pKa = 11.84VCLLLRR163 pKa = 11.84HH164 pKa = 5.42WTRR167 pKa = 11.84PPKK170 pKa = 10.66NGGKK174 pKa = 10.05RR175 pKa = 11.84GGGEE179 pKa = 3.66

Molecular weight: 19.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3700

0

3700

1196687

23

3296

323.4

35.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.641 ± 0.052

0.914 ± 0.016

6.445 ± 0.055

6.185 ± 0.039

3.629 ± 0.025

8.54 ± 0.052

2.274 ± 0.021

5.038 ± 0.035

3.893 ± 0.04

10.329 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.751 ± 0.022

2.843 ± 0.023

5.073 ± 0.036

3.327 ± 0.025

6.568 ± 0.043

5.281 ± 0.029

5.367 ± 0.037

7.221 ± 0.033

1.357 ± 0.015

2.326 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski