Pseudomonas phage phi8

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Vidaverviricetes; Mindivirales; Cystoviridae; Cystovirus; Pseudomonas virus phi8

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9MC03|Q9MC03_9VIRU p5 OS=Pseudomonas phage phi8 OX=120086 GN=5 PE=4 SV=1
MM1 pKa = 7.87TDD3 pKa = 4.48PITAVAINRR12 pKa = 11.84LVDD15 pKa = 4.19TIDD18 pKa = 3.29RR19 pKa = 11.84AAWASMEE26 pKa = 4.44DD27 pKa = 4.0EE28 pKa = 4.62EE29 pKa = 4.67KK30 pKa = 10.62LGYY33 pKa = 8.88IRR35 pKa = 11.84RR36 pKa = 11.84YY37 pKa = 10.46VSDD40 pKa = 4.68ADD42 pKa = 4.15DD43 pKa = 4.97LGISPRR49 pKa = 11.84MVDD52 pKa = 4.32DD53 pKa = 3.84VLDD56 pKa = 5.13GISSKK61 pKa = 10.74PVVQDD66 pKa = 3.49DD67 pKa = 4.26VFGEE71 pKa = 4.42AEE73 pKa = 4.3TASSLASRR81 pKa = 11.84LATTLTRR88 pKa = 11.84ATALRR93 pKa = 11.84TVMVDD98 pKa = 2.88GYY100 pKa = 9.66RR101 pKa = 11.84VVVGRR106 pKa = 11.84VGEE109 pKa = 4.21LTLSARR115 pKa = 11.84VVQEE119 pKa = 3.55DD120 pKa = 3.18HH121 pKa = 6.39RR122 pKa = 11.84QVVVGYY128 pKa = 9.94IRR130 pKa = 11.84VAHH133 pKa = 6.43DD134 pKa = 3.25VGGDD138 pKa = 3.09EE139 pKa = 4.31YY140 pKa = 11.46TRR142 pKa = 11.84MCANIARR149 pKa = 11.84NEE151 pKa = 3.68VDD153 pKa = 3.2YY154 pKa = 11.55DD155 pKa = 4.24RR156 pKa = 11.84ITIGGDD162 pKa = 3.11EE163 pKa = 4.56SSLEE167 pKa = 4.14DD168 pKa = 5.28DD169 pKa = 4.23EE170 pKa = 5.31QADD173 pKa = 3.91EE174 pKa = 4.19AA175 pKa = 5.03

Molecular weight: 19.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9MC09|Q9MC09_9VIRU p3b OS=Pseudomonas phage phi8 OX=120086 GN=3b PE=4 SV=1
MM1 pKa = 7.41RR2 pKa = 11.84QLIVDD7 pKa = 4.19EE8 pKa = 4.34QQWDD12 pKa = 3.8RR13 pKa = 11.84SSSTLMVVKK22 pKa = 10.21GIRR25 pKa = 11.84LYY27 pKa = 11.15LSYY30 pKa = 11.06DD31 pKa = 3.19GTAINVDD38 pKa = 3.59GAIGNGNLTKK48 pKa = 10.99GEE50 pKa = 4.38LFAGLYY56 pKa = 10.04PMLARR61 pKa = 11.84FPGARR66 pKa = 11.84YY67 pKa = 7.69VTWQRR72 pKa = 11.84GTRR75 pKa = 11.84SNRR78 pKa = 11.84FRR80 pKa = 11.84LRR82 pKa = 11.84NLDD85 pKa = 3.38HH86 pKa = 6.49QAVKK90 pKa = 10.69RR91 pKa = 11.84LRR93 pKa = 11.84TSAAVEE99 pKa = 3.73RR100 pKa = 11.84RR101 pKa = 11.84TDD103 pKa = 3.2CRR105 pKa = 11.84YY106 pKa = 8.98PATKK110 pKa = 10.38DD111 pKa = 3.23VTT113 pKa = 3.72

Molecular weight: 12.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19

0

19

4620

59

792

243.2

26.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.584 ± 0.49

0.411 ± 0.093

5.844 ± 0.338

5.39 ± 0.317

3.615 ± 0.335

7.511 ± 0.591

1.71 ± 0.254

5.455 ± 0.336

4.74 ± 0.407

9.545 ± 0.368

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.506 ± 0.198

3.398 ± 0.428

3.636 ± 0.235

3.485 ± 0.244

5.779 ± 0.368

7.208 ± 0.304

6.212 ± 0.405

8.312 ± 0.411

0.866 ± 0.127

2.792 ± 0.328
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski