Salmonella phage RE2010

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Peduovirinae; Felsduovirus; Salmonella virus RE2010

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E5G6N4|E5G6N4_9CAUD Tail completion protein OS=Salmonella phage RE2010 OX=929814 GN=ELphiS00026 PE=4 SV=1
MM1 pKa = 7.65NKK3 pKa = 9.37PQSLRR8 pKa = 11.84NALNKK13 pKa = 9.77AVPYY17 pKa = 10.26VRR19 pKa = 11.84NNPDD23 pKa = 3.44KK24 pKa = 11.34LHH26 pKa = 6.71LFVDD30 pKa = 3.87NGSLVATGASSMSWEE45 pKa = 3.73YY46 pKa = 10.8RR47 pKa = 11.84YY48 pKa = 8.2TLNVVIEE55 pKa = 4.75DD56 pKa = 3.96FSGDD60 pKa = 3.63QNLLMAPVLLWLSASQPDD78 pKa = 4.55AINNPEE84 pKa = 4.05LRR86 pKa = 11.84EE87 pKa = 3.86KK88 pKa = 11.11LFTFDD93 pKa = 4.91VDD95 pKa = 3.11ILRR98 pKa = 11.84NDD100 pKa = 3.17VCDD103 pKa = 3.48ISLNLQLTEE112 pKa = 3.8RR113 pKa = 11.84VLVNTDD119 pKa = 3.21GSVSSVEE126 pKa = 4.44AVTEE130 pKa = 3.94PDD132 pKa = 3.47EE133 pKa = 4.56PEE135 pKa = 4.08EE136 pKa = 3.84MWTVKK141 pKa = 10.42RR142 pKa = 11.84GG143 pKa = 3.24

Molecular weight: 16.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E5G6N5|E5G6N5_9CAUD Uncharacterized protein OS=Salmonella phage RE2010 OX=929814 GN=ELphiS00027c PE=4 SV=1
MM1 pKa = 8.11DD2 pKa = 5.33DD3 pKa = 3.26WLMALLANLEE13 pKa = 4.01PTARR17 pKa = 11.84SRR19 pKa = 11.84MMRR22 pKa = 11.84QLAQQLRR29 pKa = 11.84RR30 pKa = 11.84SQQQNIRR37 pKa = 11.84LQRR40 pKa = 11.84NPDD43 pKa = 3.01GSGYY47 pKa = 9.06EE48 pKa = 3.95PRR50 pKa = 11.84RR51 pKa = 11.84VTARR55 pKa = 11.84SKK57 pKa = 10.21KK58 pKa = 10.29GRR60 pKa = 11.84IKK62 pKa = 10.53RR63 pKa = 11.84QMFAKK68 pKa = 10.4LRR70 pKa = 11.84TTKK73 pKa = 10.41YY74 pKa = 10.55LKK76 pKa = 9.46TAASADD82 pKa = 3.84SASVQFDD89 pKa = 3.38GKK91 pKa = 7.31VQRR94 pKa = 11.84IARR97 pKa = 11.84VHH99 pKa = 6.27HH100 pKa = 5.92YY101 pKa = 10.35GLRR104 pKa = 11.84DD105 pKa = 3.15RR106 pKa = 11.84VSRR109 pKa = 11.84KK110 pKa = 9.9GPEE113 pKa = 3.26VRR115 pKa = 11.84YY116 pKa = 10.29AEE118 pKa = 4.09RR119 pKa = 11.84HH120 pKa = 5.03LLGINDD126 pKa = 3.67EE127 pKa = 4.57VAALTCDD134 pKa = 3.18TLLRR138 pKa = 11.84WLIAA142 pKa = 3.88

Molecular weight: 16.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

10250

38

935

218.1

24.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.688 ± 0.742

1.122 ± 0.182

5.854 ± 0.296

6.263 ± 0.354

3.288 ± 0.238

6.293 ± 0.428

1.951 ± 0.226

5.249 ± 0.348

5.454 ± 0.298

9.756 ± 0.317

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.654 ± 0.146

4.546 ± 0.238

4.254 ± 0.213

4.176 ± 0.275

6.585 ± 0.359

6.38 ± 0.234

5.659 ± 0.398

6.498 ± 0.339

1.444 ± 0.145

2.888 ± 0.263
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski