Influenza A virus (A/duck/Jiangxi/5460/2014(mixed))

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus; Influenza A virus; mixed subtypes

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UVB0|A0A0B4UVB0_9INFA Isoform of A0A0B4UWC0 Neuraminidase OS=Influenza A virus (A/duck/Jiangxi/5460/2014(mixed)) OX=1587846 GN=NA PE=3 SV=1
MM1 pKa = 7.38SLLTEE6 pKa = 4.4VEE8 pKa = 4.38TPTRR12 pKa = 11.84NGWEE16 pKa = 4.5CKK18 pKa = 10.41CNDD21 pKa = 3.43SSDD24 pKa = 4.01PLVIAASIIGILHH37 pKa = 7.1LILWILDD44 pKa = 3.39RR45 pKa = 11.84LFFKK49 pKa = 10.78CIYY52 pKa = 9.95RR53 pKa = 11.84RR54 pKa = 11.84LKK56 pKa = 10.55YY57 pKa = 10.03GLKK60 pKa = 10.09RR61 pKa = 11.84GPSTEE66 pKa = 4.02GVPEE70 pKa = 4.24SMRR73 pKa = 11.84EE74 pKa = 3.92EE75 pKa = 4.1YY76 pKa = 10.41RR77 pKa = 11.84QEE79 pKa = 3.74QQSAVDD85 pKa = 3.3VDD87 pKa = 4.47DD88 pKa = 3.92GHH90 pKa = 6.42FVNIEE95 pKa = 3.83LEE97 pKa = 4.08

Molecular weight: 11.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UW38|A0A0B4UW38_9INFA Matrix protein 1 OS=Influenza A virus (A/duck/Jiangxi/5460/2014(mixed)) OX=1587846 GN=M1 PE=3 SV=1
MM1 pKa = 7.36EE2 pKa = 6.17RR3 pKa = 11.84IKK5 pKa = 10.51EE6 pKa = 4.0LRR8 pKa = 11.84DD9 pKa = 3.29LMSQSRR15 pKa = 11.84TRR17 pKa = 11.84EE18 pKa = 3.86ILTKK22 pKa = 8.81TTVDD26 pKa = 2.86HH27 pKa = 5.76MAIIKK32 pKa = 10.06KK33 pKa = 7.84YY34 pKa = 8.29TSGRR38 pKa = 11.84QEE40 pKa = 3.99KK41 pKa = 10.64NPALRR46 pKa = 11.84MKK48 pKa = 10.19WMMAMKK54 pKa = 10.45YY55 pKa = 10.07PITADD60 pKa = 3.19KK61 pKa = 10.67RR62 pKa = 11.84IMEE65 pKa = 4.94MIPEE69 pKa = 4.3RR70 pKa = 11.84NEE72 pKa = 3.52QGQTLWSKK80 pKa = 9.91TNDD83 pKa = 3.44AGSDD87 pKa = 3.19RR88 pKa = 11.84VMVSPLAVTWWNRR101 pKa = 11.84NGPTTSTVHH110 pKa = 5.17YY111 pKa = 8.49PKK113 pKa = 10.47VYY115 pKa = 8.68KK116 pKa = 10.24TYY118 pKa = 10.19FEE120 pKa = 3.9KK121 pKa = 11.0AEE123 pKa = 4.08RR124 pKa = 11.84LKK126 pKa = 10.93HH127 pKa = 4.77GTFGPVHH134 pKa = 6.7FRR136 pKa = 11.84NQVKK140 pKa = 9.47IRR142 pKa = 11.84RR143 pKa = 11.84RR144 pKa = 11.84VDD146 pKa = 3.11INPGHH151 pKa = 7.33ADD153 pKa = 3.84LSAKK157 pKa = 9.4EE158 pKa = 3.98AQDD161 pKa = 3.52VIMEE165 pKa = 4.23VVFPNEE171 pKa = 3.21VGARR175 pKa = 11.84ILTSEE180 pKa = 4.22SQLTITKK187 pKa = 9.33EE188 pKa = 4.15KK189 pKa = 10.77KK190 pKa = 10.27EE191 pKa = 3.94EE192 pKa = 3.95LQDD195 pKa = 3.83CKK197 pKa = 10.5IAPLMVAYY205 pKa = 9.47MLEE208 pKa = 4.19RR209 pKa = 11.84EE210 pKa = 4.59LVRR213 pKa = 11.84KK214 pKa = 6.95XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXVLFQNWGVEE452 pKa = 4.28PIDD455 pKa = 3.95NVMGMIGILPDD466 pKa = 3.56MTPSTEE472 pKa = 3.52MSLRR476 pKa = 11.84GVRR479 pKa = 11.84VSKK482 pKa = 10.41MGVDD486 pKa = 4.11EE487 pKa = 4.4YY488 pKa = 11.53SSTEE492 pKa = 3.81RR493 pKa = 11.84VVVNIDD499 pKa = 2.92RR500 pKa = 11.84FLRR503 pKa = 11.84VRR505 pKa = 11.84DD506 pKa = 3.41QRR508 pKa = 11.84GNILLSPEE516 pKa = 4.37EE517 pKa = 3.98VSEE520 pKa = 4.32TQGTEE525 pKa = 3.64KK526 pKa = 10.4LTITYY531 pKa = 9.18SSSMMWEE538 pKa = 3.7INGPEE543 pKa = 3.98SVLVNTYY550 pKa = 7.57QWIIRR555 pKa = 11.84NWEE558 pKa = 3.94TVKK561 pKa = 10.56IQWSQDD567 pKa = 2.67PTMLYY572 pKa = 10.81NKK574 pKa = 9.63MEE576 pKa = 4.24FEE578 pKa = 4.66PFQSLVPKK586 pKa = 10.24AARR589 pKa = 11.84GQYY592 pKa = 10.02SGFVRR597 pKa = 11.84TLFQQMRR604 pKa = 11.84DD605 pKa = 3.48VLGTFDD611 pKa = 3.43TVQIIKK617 pKa = 10.36LLPFAAAPPEE627 pKa = 3.92QSRR630 pKa = 11.84MQFSSLTVNVRR641 pKa = 11.84GSGMRR646 pKa = 11.84ILVRR650 pKa = 11.84GNSPVFNYY658 pKa = 10.56NKK660 pKa = 8.71ATKK663 pKa = 10.14RR664 pKa = 11.84LTVLGKK670 pKa = 10.13DD671 pKa = 3.2AGALTEE677 pKa = 5.36DD678 pKa = 4.62PDD680 pKa = 3.67EE681 pKa = 4.45GTXGVEE687 pKa = 4.06SAVLRR692 pKa = 11.84GFLILGKK699 pKa = 9.47EE700 pKa = 4.26DD701 pKa = 3.46KK702 pKa = 10.5RR703 pKa = 11.84YY704 pKa = 10.65GPALSINEE712 pKa = 4.34LSNLAKK718 pKa = 10.63GEE720 pKa = 4.09KK721 pKa = 10.32ANVLIGQGDD730 pKa = 3.9VVLVMKK736 pKa = 10.39RR737 pKa = 11.84KK738 pKa = 9.29RR739 pKa = 11.84DD740 pKa = 3.62SSILTDD746 pKa = 3.31SQTATKK752 pKa = 9.73RR753 pKa = 11.84IRR755 pKa = 11.84MAINN759 pKa = 3.15

Molecular weight: 60.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

2

14

5838

90

759

417.0

45.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.379 ± 0.417

2.073 ± 0.406

4.693 ± 0.297

6.903 ± 0.641

3.888 ± 0.306

6.629 ± 0.573

1.627 ± 0.172

6.492 ± 0.475

5.464 ± 0.381

7.211 ± 0.494

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.374 ± 0.399

5.344 ± 0.55

3.803 ± 0.251

3.905 ± 0.368

6.355 ± 0.525

6.869 ± 0.319

6.475 ± 0.384

5.396 ± 0.399

1.679 ± 0.178

2.467 ± 0.209
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski