Alcaligenaceae bacterium 429

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; unclassified Alcaligenaceae

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3405 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4S1E732|A0A4S1E732_9BURK CoA transferase subunit A OS=Alcaligenaceae bacterium 429 OX=2562948 GN=E4695_14860 PE=3 SV=1
MM1 pKa = 7.71PLSFKK6 pKa = 10.76QRR8 pKa = 11.84FCAGLPQTPPEE19 pKa = 5.05LDD21 pKa = 3.44LQLDD25 pKa = 3.73AFIVFEE31 pKa = 4.6SEE33 pKa = 4.65SLPDD37 pKa = 6.44DD38 pKa = 3.63IDD40 pKa = 5.84ADD42 pKa = 4.02AKK44 pKa = 10.82QLLLAEE50 pKa = 5.03GLPEE54 pKa = 4.09SAPPFLSFSTYY65 pKa = 9.22STDD68 pKa = 4.53DD69 pKa = 3.49IEE71 pKa = 4.57SLRR74 pKa = 11.84AFGDD78 pKa = 3.38IPASFFPLGQTAYY91 pKa = 10.66GDD93 pKa = 3.91LLGIDD98 pKa = 4.12TDD100 pKa = 3.95TKK102 pKa = 10.29EE103 pKa = 4.06IIYY106 pKa = 10.3FNHH109 pKa = 7.17DD110 pKa = 3.47NNNQRR115 pKa = 11.84VFINTSLAQFLEE127 pKa = 4.59CLCVYY132 pKa = 10.2QEE134 pKa = 4.22HH135 pKa = 6.43LHH137 pKa = 6.74AEE139 pKa = 4.42THH141 pKa = 5.87TSPLDD146 pKa = 4.26AIEE149 pKa = 4.6QVDD152 pKa = 4.48APAAQPGTMWHH163 pKa = 6.26TEE165 pKa = 3.9ALSDD169 pKa = 3.88

Molecular weight: 18.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4S1EGM0|A0A4S1EGM0_9BURK ABC transporter ATP-binding protein OS=Alcaligenaceae bacterium 429 OX=2562948 GN=E4695_03010 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.06QPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.17RR14 pKa = 11.84THH16 pKa = 5.95GFRR19 pKa = 11.84IRR21 pKa = 11.84MKK23 pKa = 9.32TRR25 pKa = 11.84TGRR28 pKa = 11.84AVLNARR34 pKa = 11.84RR35 pKa = 11.84GKK37 pKa = 9.63GRR39 pKa = 11.84KK40 pKa = 8.91RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3405

0

3405

1057531

26

2496

310.6

34.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.632 ± 0.047

0.946 ± 0.015

5.029 ± 0.03

5.611 ± 0.044

3.67 ± 0.026

7.519 ± 0.046

2.363 ± 0.026

5.395 ± 0.03

3.548 ± 0.029

10.956 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.677 ± 0.022

3.252 ± 0.027

4.63 ± 0.026

4.781 ± 0.041

5.642 ± 0.035

6.23 ± 0.031

5.387 ± 0.03

7.605 ± 0.043

1.453 ± 0.019

2.675 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski