Paenibacillus sp. NFR01

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; unclassified Paenibacillus

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4792 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I0CM81|A0A1I0CM81_9BACL Glycosyl hydrolases family 2 TIM barrel domain OS=Paenibacillus sp. NFR01 OX=1566279 GN=SAMN03159358_1017 PE=3 SV=1
GG1 pKa = 6.27ATGDD5 pKa = 3.73AGATGATGATGDD17 pKa = 3.92TGATGATGATGDD29 pKa = 4.07AGATGATGATGDD41 pKa = 4.07AGATGATGDD50 pKa = 3.92TGATGATGVTGPTGDD65 pKa = 3.55TGVTGATGVTGITGATGDD83 pKa = 4.01TGPTGLTGATGATGITGATGDD104 pKa = 3.76TGATGLTGATGATGTTFTTEE124 pKa = 3.75NFSALLPTISTSTASQLTGWSVASPYY150 pKa = 9.69YY151 pKa = 10.77ASAAFNPTTGNYY163 pKa = 7.4TVPEE167 pKa = 3.93TGRR170 pKa = 11.84YY171 pKa = 8.99SFEE174 pKa = 3.91ATLSYY179 pKa = 10.32ATTASITISLGAGVNPAFVIRR200 pKa = 11.84RR201 pKa = 11.84TSPVVTDD208 pKa = 4.17LVSGLFPLLDD218 pKa = 3.58VNVALVLTLRR228 pKa = 11.84AVLGNGTVTLTGEE241 pKa = 4.3FEE243 pKa = 4.8LNAGDD248 pKa = 4.03VIGVFYY254 pKa = 11.03VADD257 pKa = 4.23GLTIPIDD264 pKa = 3.22IGGDD268 pKa = 3.4STGSVWSVHH277 pKa = 5.83RR278 pKa = 11.84LSS280 pKa = 3.91

Molecular weight: 26.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I0JFM1|A0A1I0JFM1_9BACL Serine protease Do OS=Paenibacillus sp. NFR01 OX=1566279 GN=SAMN03159358_3230 PE=3 SV=1
MM1 pKa = 7.9RR2 pKa = 11.84PTFKK6 pKa = 10.82PNVSKK11 pKa = 10.8RR12 pKa = 11.84KK13 pKa = 8.79KK14 pKa = 8.3VHH16 pKa = 5.4GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.87VLAARR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.2GRR39 pKa = 11.84KK40 pKa = 8.79VLSAA44 pKa = 4.05

Molecular weight: 5.06 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4792

0

4792

1577750

26

2520

329.2

36.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.519 ± 0.05

0.738 ± 0.011

5.043 ± 0.026

6.7 ± 0.041

3.971 ± 0.025

8.104 ± 0.035

1.994 ± 0.018

6.128 ± 0.034

4.885 ± 0.035

10.436 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.696 ± 0.022

3.547 ± 0.031

4.228 ± 0.027

3.6 ± 0.02

5.302 ± 0.039

6.1 ± 0.033

5.376 ± 0.039

6.982 ± 0.025

1.219 ± 0.015

3.433 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski