Sclerophthora macrospora virus B

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 7.67

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|Q9WTA1|Q9WTA1_9VIRU Polyprotein OS=Sclerophthora macrospora virus B OX=75914 PE=4 SV=1
MM1 pKa = 7.42EE2 pKa = 6.21PKK4 pKa = 9.27TQVNYY9 pKa = 11.13AEE11 pKa = 4.53VVGGRR16 pKa = 11.84YY17 pKa = 8.67NRR19 pKa = 11.84PAEE22 pKa = 3.99VRR24 pKa = 11.84AMRR27 pKa = 11.84FLASNLYY34 pKa = 9.88GAGIVFDD41 pKa = 3.96ATEE44 pKa = 4.43GGAKK48 pKa = 10.09CFTSPPPHH56 pKa = 6.66PASLRR61 pKa = 11.84FCSVRR66 pKa = 11.84GMPALAVCQTQGWWASFVLKK86 pKa = 10.51SLPFPARR93 pKa = 11.84WLIFQFLNYY102 pKa = 8.76CWEE105 pKa = 4.0WVEE108 pKa = 3.96LQDD111 pKa = 4.46RR112 pKa = 11.84SAVFTATTWTLLPMAKK128 pKa = 9.43RR129 pKa = 11.84FMGPGLEE136 pKa = 4.09YY137 pKa = 10.61PEE139 pKa = 5.73IGEE142 pKa = 4.29DD143 pKa = 2.92WFDD146 pKa = 5.55RR147 pKa = 11.84IIDD150 pKa = 3.74SPRR153 pKa = 11.84YY154 pKa = 9.28RR155 pKa = 11.84GWEE158 pKa = 3.81VVPQVSYY165 pKa = 10.9LYY167 pKa = 10.68QNVGNCCLSYY177 pKa = 10.42SAHH180 pKa = 5.82EE181 pKa = 4.6KK182 pKa = 9.81IVAFVTEE189 pKa = 4.95LYY191 pKa = 10.75CRR193 pKa = 11.84FSTYY197 pKa = 10.78GIYY200 pKa = 10.67LSARR204 pKa = 11.84TLRR207 pKa = 11.84QLSHH211 pKa = 4.89VHH213 pKa = 6.26HH214 pKa = 6.86IKK216 pKa = 10.84YY217 pKa = 9.23MVMLQLVRR225 pKa = 11.84SVPVARR231 pKa = 11.84ALSTGDD237 pKa = 3.23AWDD240 pKa = 3.97LSLVFARR247 pKa = 11.84FLEE250 pKa = 4.47WEE252 pKa = 4.34RR253 pKa = 11.84RR254 pKa = 11.84WSLSLLDD261 pKa = 5.99LIAPYY266 pKa = 11.12LNFAWRR272 pKa = 11.84DD273 pKa = 3.74CVSPTLVKK281 pKa = 10.08TDD283 pKa = 3.2VSLGMSCSKK292 pKa = 9.64GTLVTFPSTLSLEE305 pKa = 4.72FSQGLKK311 pKa = 10.67FNVFEE316 pKa = 4.51PSEE319 pKa = 4.23LCCEE323 pKa = 4.33EE324 pKa = 3.93RR325 pKa = 11.84HH326 pKa = 6.23HH327 pKa = 6.9PLLTVSMCLAYY338 pKa = 10.73VLLWYY343 pKa = 10.21LIIQLVIISADD354 pKa = 3.52VVLDD358 pKa = 3.61ASKK361 pKa = 10.68YY362 pKa = 8.09ICEE365 pKa = 4.37TSLHH369 pKa = 5.06VCQRR373 pKa = 11.84FATLLSSLLRR383 pKa = 11.84WYY385 pKa = 10.09RR386 pKa = 11.84LKK388 pKa = 10.22IDD390 pKa = 3.5RR391 pKa = 11.84SLVRR395 pKa = 11.84DD396 pKa = 3.54SRR398 pKa = 11.84VVTSCSGEE406 pKa = 3.89APPVYY411 pKa = 10.05RR412 pKa = 11.84LVSKK416 pKa = 10.85GPEE419 pKa = 3.93AVDD422 pKa = 3.48SLEE425 pKa = 3.57MSMRR429 pKa = 11.84GSEE432 pKa = 3.66IRR434 pKa = 11.84TSVAIPGCLYY444 pKa = 10.57LAVVHH449 pKa = 7.0DD450 pKa = 4.47SDD452 pKa = 5.2VIFIGMAFRR461 pKa = 11.84YY462 pKa = 9.44KK463 pKa = 10.64GYY465 pKa = 10.62LVTAQHH471 pKa = 6.81NILAMSSAPGKK482 pKa = 10.79YY483 pKa = 8.06YY484 pKa = 10.7VLPFRR489 pKa = 11.84PGKK492 pKa = 10.27DD493 pKa = 3.2SEE495 pKa = 4.34FCYY498 pKa = 10.6LDD500 pKa = 3.31QEE502 pKa = 4.54RR503 pKa = 11.84MIEE506 pKa = 4.08LTSVMLHH513 pKa = 5.97NDD515 pKa = 2.68IVSEE519 pKa = 4.09EE520 pKa = 3.92FQGYY524 pKa = 9.47DD525 pKa = 3.18VAIIPIAAAAWSCLGVKK542 pKa = 10.12SLLHH546 pKa = 7.26ADD548 pKa = 3.68ATWGINVTLYY558 pKa = 11.14GLEE561 pKa = 3.97KK562 pKa = 9.26TGKK565 pKa = 9.88RR566 pKa = 11.84KK567 pKa = 9.0LQRR570 pKa = 11.84SLGTIRR576 pKa = 11.84EE577 pKa = 4.33DD578 pKa = 3.89KK579 pKa = 10.49EE580 pKa = 4.6SPLHH584 pKa = 4.99SVYY587 pKa = 11.19YY588 pKa = 10.02NASTLRR594 pKa = 11.84GWSGSPVLNGHH605 pKa = 5.9KK606 pKa = 9.97RR607 pKa = 11.84VVALHH612 pKa = 6.78CGTNGQVNRR621 pKa = 11.84GLNFAFVRR629 pKa = 11.84FIIEE633 pKa = 3.37IHH635 pKa = 6.5EE636 pKa = 4.34INTRR640 pKa = 11.84EE641 pKa = 4.07SSTDD645 pKa = 3.08SYY647 pKa = 11.37EE648 pKa = 4.57DD649 pKa = 3.97EE650 pKa = 4.19IEE652 pKa = 3.97KK653 pKa = 10.72SFRR656 pKa = 11.84TRR658 pKa = 11.84GGYY661 pKa = 9.8LSRR664 pKa = 11.84RR665 pKa = 11.84QEE667 pKa = 3.89EE668 pKa = 4.61LEE670 pKa = 3.86VEE672 pKa = 4.13RR673 pKa = 11.84RR674 pKa = 11.84VDD676 pKa = 3.25EE677 pKa = 5.08GIAVYY682 pKa = 10.19KK683 pKa = 10.28RR684 pKa = 11.84YY685 pKa = 9.59IVSQSSRR692 pKa = 11.84GIISFCDD699 pKa = 2.88SDD701 pKa = 4.05YY702 pKa = 11.39VDD704 pKa = 3.67RR705 pKa = 11.84QRR707 pKa = 11.84ATHH710 pKa = 6.05TATTNVVEE718 pKa = 5.37QVFDD722 pKa = 4.61DD723 pKa = 4.05NLEE726 pKa = 4.01SSYY729 pKa = 11.35SPKK732 pKa = 10.41CIQRR736 pKa = 11.84CRR738 pKa = 11.84TGEE741 pKa = 3.98AEE743 pKa = 4.04KK744 pKa = 11.2SEE746 pKa = 4.49DD747 pKa = 3.68LSSAEE752 pKa = 4.26QLFDD756 pKa = 3.64YY757 pKa = 10.88HH758 pKa = 8.62DD759 pKa = 4.58GDD761 pKa = 4.01PFVASRR767 pKa = 11.84VTTRR771 pKa = 11.84LSAPHH776 pKa = 5.56YY777 pKa = 9.01KK778 pKa = 10.17GRR780 pKa = 11.84PEE782 pKa = 4.59RR783 pKa = 11.84YY784 pKa = 9.23DD785 pKa = 3.21EE786 pKa = 4.94HH787 pKa = 8.47VDD789 pKa = 3.59LSRR792 pKa = 11.84AKK794 pKa = 10.26EE795 pKa = 3.79LGYY798 pKa = 10.86DD799 pKa = 3.54PEE801 pKa = 4.54EE802 pKa = 4.96YY803 pKa = 11.04GMPRR807 pKa = 11.84ATDD810 pKa = 3.17RR811 pKa = 11.84TSAIKK816 pKa = 10.34RR817 pKa = 11.84SAASLSKK824 pKa = 10.72SLEE827 pKa = 3.88DD828 pKa = 5.16AIRR831 pKa = 11.84TSKK834 pKa = 9.62FQKK837 pKa = 9.77PSEE840 pKa = 4.36DD841 pKa = 3.32LRR843 pKa = 11.84SAAVSIMMDD852 pKa = 3.4TLSSLRR858 pKa = 11.84YY859 pKa = 8.36PVDD862 pKa = 3.31GSGVTRR868 pKa = 11.84DD869 pKa = 4.23KK870 pKa = 10.89IASQLNSSAVGDD882 pKa = 3.91GRR884 pKa = 11.84SPGLPYY890 pKa = 10.68ASEE893 pKa = 4.36FPTNRR898 pKa = 11.84DD899 pKa = 3.36LLSKK903 pKa = 10.51FSVDD907 pKa = 3.16EE908 pKa = 4.26LADD911 pKa = 4.68LIYY914 pKa = 10.58TKK916 pKa = 10.62YY917 pKa = 10.53RR918 pKa = 11.84EE919 pKa = 4.51DD920 pKa = 3.11RR921 pKa = 11.84WEE923 pKa = 3.89QPSINFLKK931 pKa = 10.42IEE933 pKa = 3.97PTKK936 pKa = 10.78VEE938 pKa = 4.59KK939 pKa = 10.65LDD941 pKa = 3.58QGLDD945 pKa = 3.08RR946 pKa = 11.84CVQAVGLDD954 pKa = 3.31TQLYY958 pKa = 7.66FRR960 pKa = 11.84CHH962 pKa = 6.13FGALADD968 pKa = 3.84VASANYY974 pKa = 9.43RR975 pKa = 11.84KK976 pKa = 10.15SPVMQGWSPLKK987 pKa = 10.61PGDD990 pKa = 3.24GHH992 pKa = 6.15YY993 pKa = 10.24LYY995 pKa = 11.18NVITRR1000 pKa = 11.84NSNRR1004 pKa = 11.84KK1005 pKa = 8.52ILEE1008 pKa = 3.81YY1009 pKa = 10.38DD1010 pKa = 3.64GKK1012 pKa = 10.21AFEE1015 pKa = 4.78YY1016 pKa = 10.31VAHH1019 pKa = 6.03TAEE1022 pKa = 4.86AYY1024 pKa = 10.85SDD1026 pKa = 3.73VTDD1029 pKa = 4.23VIIGLSTPAVGANVDD1044 pKa = 3.91KK1045 pKa = 10.88VRR1047 pKa = 11.84EE1048 pKa = 4.2WRR1050 pKa = 11.84SEE1052 pKa = 3.59AQRR1055 pKa = 11.84FMMRR1059 pKa = 11.84VGEE1062 pKa = 4.18SGYY1065 pKa = 11.24LLADD1069 pKa = 3.39GTLVSKK1075 pKa = 10.8RR1076 pKa = 11.84SPFVLSSGRR1085 pKa = 11.84WDD1087 pKa = 3.34TFLRR1091 pKa = 11.84NSLTGYY1097 pKa = 8.16YY1098 pKa = 9.32WLIIGLLDD1106 pKa = 4.29AGYY1109 pKa = 8.15TADD1112 pKa = 5.05DD1113 pKa = 3.73IKK1115 pKa = 11.48AKK1117 pKa = 10.46FLIKK1121 pKa = 10.72VGGDD1125 pKa = 3.56DD1126 pKa = 4.53VILSVPSNFDD1136 pKa = 3.22TQTFTSALGRR1146 pKa = 11.84YY1147 pKa = 7.66GMKK1150 pKa = 9.2IHH1152 pKa = 6.72KK1153 pKa = 9.99VKK1155 pKa = 10.76LKK1157 pKa = 10.52PITDD1161 pKa = 3.67DD1162 pKa = 4.42FEE1164 pKa = 4.42FFSWKK1169 pKa = 9.68FSKK1172 pKa = 10.62NSRR1175 pKa = 11.84GLVQWTPTRR1184 pKa = 11.84FSKK1187 pKa = 10.81HH1188 pKa = 5.81LEE1190 pKa = 3.74NFFNTKK1196 pKa = 10.24HH1197 pKa = 6.38EE1198 pKa = 4.65DD1199 pKa = 2.9RR1200 pKa = 11.84HH1201 pKa = 5.2QALVSHH1207 pKa = 6.22MMNWVHH1213 pKa = 6.91SEE1215 pKa = 3.27PHH1217 pKa = 5.82YY1218 pKa = 11.09NFFRR1222 pKa = 11.84EE1223 pKa = 3.65IYY1225 pKa = 8.02MRR1227 pKa = 11.84GRR1229 pKa = 11.84EE1230 pKa = 4.32SEE1232 pKa = 3.88PDD1234 pKa = 2.96IYY1236 pKa = 10.6EE1237 pKa = 3.8LSKK1240 pKa = 10.63IPEE1243 pKa = 3.97RR1244 pKa = 11.84RR1245 pKa = 11.84DD1246 pKa = 3.02LLFYY1250 pKa = 11.08LRR1252 pKa = 11.84GNEE1255 pKa = 4.6SVIKK1259 pKa = 10.41DD1260 pKa = 3.55WGSVGAFVSLASAAVLKK1277 pKa = 11.14NNN1279 pKa = 4.11

Molecular weight:
145.09 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|Q9WTA1|Q9WTA1_9VIRU Polyprotein OS=Sclerophthora macrospora virus B OX=75914 PE=4 SV=1
MM1 pKa = 7.14TKK3 pKa = 10.66KK4 pKa = 10.21NVKK7 pKa = 8.41TSGAIVVYY15 pKa = 10.23KK16 pKa = 9.88PQTRR20 pKa = 11.84LPPQNKK26 pKa = 9.19KK27 pKa = 10.58KK28 pKa = 10.25NRR30 pKa = 11.84KK31 pKa = 8.29KK32 pKa = 10.36KK33 pKa = 10.3GRR35 pKa = 11.84VGPGKK40 pKa = 10.53SDD42 pKa = 3.14MLACYY47 pKa = 9.26RR48 pKa = 11.84ASLSAPFSITAQGARR63 pKa = 11.84VPDD66 pKa = 4.29MYY68 pKa = 11.21SCPTVTRR75 pKa = 11.84HH76 pKa = 4.26ITKK79 pKa = 10.36SFTVVTNSAGEE90 pKa = 3.89ADD92 pKa = 4.47LVVLPSAFHH101 pKa = 6.32HH102 pKa = 6.03AVSPRR107 pKa = 11.84NNIPSGVTWSTLSGATAANALVFTSPGQLANSLVNYY143 pKa = 9.65RR144 pKa = 11.84IVGYY148 pKa = 7.74GVRR151 pKa = 11.84IFGVASMTNTAGRR164 pKa = 11.84AVIATVPIASWINDD178 pKa = 3.03KK179 pKa = 9.49TASVGGQVSNAVNAAANVAGTLVAYY204 pKa = 7.58GVPVTGAYY212 pKa = 10.18VDD214 pKa = 3.81IASLPSLPNTVGVSMMNLSEE234 pKa = 4.24RR235 pKa = 11.84QMIITPKK242 pKa = 8.87ITGPEE247 pKa = 3.37AFVFNEE253 pKa = 4.19TTDD256 pKa = 3.34NAIGFNIVDD265 pKa = 3.6QTSVSFVASGDD276 pKa = 3.37ASYY279 pKa = 11.38LRR281 pKa = 11.84VAGFEE286 pKa = 4.16GVVIGLSGCPPNVGVLEE303 pKa = 4.3VEE305 pKa = 4.36LVYY308 pKa = 10.73HH309 pKa = 6.93LEE311 pKa = 4.26GQPTITNNNIVGDD324 pKa = 4.17SPEE327 pKa = 4.31SFCAPVSFLNTLQAIAKK344 pKa = 7.77TPTFKK349 pKa = 10.59QGLKK353 pKa = 10.25VVGNSIYY360 pKa = 10.39PGLGTLASRR369 pKa = 11.84YY370 pKa = 9.18

Molecular weight:
38.67 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1649

370

1279

824.5

91.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.58 ± 1.376

1.698 ± 0.316

4.912 ± 1.269

5.276 ± 1.455

4.306 ± 0.405

6.671 ± 1.15

2.062 ± 0.502

4.791 ± 0.315

4.488 ± 0.055

8.914 ± 1.381

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.941 ± 0.025

3.76 ± 1.534

4.73 ± 0.899

2.668 ± 0.018

6.367 ± 1.46

9.46 ± 0.277

5.761 ± 1.339

8.611 ± 1.679

1.698 ± 0.592

4.306 ± 0.682

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski