Burkholderiales bacterium PBB5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; unclassified Burkholderiales

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8079 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257LQ16|A0A257LQ16_9BURK Hybrid-cluster NAD(P)-dependent oxidoreductase OS=Burkholderiales bacterium PBB5 OX=2015567 GN=CFE45_04455 PE=4 SV=1
MM1 pKa = 7.55SMSLSKK7 pKa = 9.65TLQWAASAALGAALCLPVAGQASVLARR34 pKa = 11.84VDD36 pKa = 4.69FEE38 pKa = 4.32AAPPLTVVGPGQSDD52 pKa = 3.22ASYY55 pKa = 10.86SEE57 pKa = 3.9AGFRR61 pKa = 11.84FTPSGGDD68 pKa = 3.38AQVDD72 pKa = 3.74LSFCSAPDD80 pKa = 3.64FCAVGNATQYY90 pKa = 9.57LTALNDD96 pKa = 3.61ASVTIAAEE104 pKa = 4.29SGSSFSLGSFEE115 pKa = 5.33ASFVPSPSLDD125 pKa = 3.5YY126 pKa = 11.25SGLSILLTLSGALSGGGTASQTFEE150 pKa = 4.55LLGTGAGDD158 pKa = 3.98YY159 pKa = 10.8LGSACLSDD167 pKa = 3.24PTYY170 pKa = 11.14INDD173 pKa = 3.92AQFALDD179 pKa = 3.78NLAFVPEE186 pKa = 5.02PSALWLVGLALAGLGLTRR204 pKa = 11.84RR205 pKa = 11.84TASRR209 pKa = 3.31

Molecular weight: 21.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257LGA0|A0A257LGA0_9BURK Ubiquinol oxidase subunit 2 OS=Burkholderiales bacterium PBB5 OX=2015567 GN=cyoA PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSQVV45 pKa = 2.54

Molecular weight: 5.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8079

0

8079

1874526

17

1946

232.0

24.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.807 ± 0.045

0.921 ± 0.01

5.256 ± 0.02

4.261 ± 0.023

3.071 ± 0.018

8.802 ± 0.028

2.354 ± 0.018

3.369 ± 0.02

2.354 ± 0.022

11.599 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.18 ± 0.013

2.261 ± 0.017

5.758 ± 0.028

4.541 ± 0.025

7.15 ± 0.031

4.95 ± 0.029

5.202 ± 0.029

7.692 ± 0.028

1.607 ± 0.013

1.864 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski