Enterobacteria phage 2851

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pankowvirus; Enterobacteria virus 2851

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 75 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B6ETB1|B6ETB1_9CAUD Putative anti-termination protein N OS=Enterobacteria phage 2851 OX=254397 GN=antN PE=4 SV=1
MM1 pKa = 7.62EE2 pKa = 4.36FHH4 pKa = 7.21EE5 pKa = 5.34SAICDD10 pKa = 3.52FRR12 pKa = 11.84ANANSVKK19 pKa = 9.25PQPIAVLFKK28 pKa = 10.94TMGAWAVLCFAADD41 pKa = 3.61DD42 pKa = 3.83TDD44 pKa = 3.42ARR46 pKa = 11.84MAIGQEE52 pKa = 4.02MEE54 pKa = 3.99MDD56 pKa = 3.55PTNDD60 pKa = 2.86EE61 pKa = 4.58FIIYY65 pKa = 7.71GAPSNYY71 pKa = 10.43LLDD74 pKa = 3.47TCNIYY79 pKa = 10.99NKK81 pKa = 10.37AAA83 pKa = 3.64

Molecular weight: 9.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B6ETF3|B6ETF3_9CAUD Uncharacterized protein OS=Enterobacteria phage 2851 OX=254397 GN=EP2851_58 PE=4 SV=1
MM1 pKa = 7.8IEE3 pKa = 4.08TLLDD7 pKa = 3.78FSGLEE12 pKa = 4.36DD13 pKa = 3.89ISRR16 pKa = 11.84DD17 pKa = 3.57LQLLSGAEE25 pKa = 3.95NNRR28 pKa = 11.84VLRR31 pKa = 11.84EE32 pKa = 4.01ATRR35 pKa = 11.84AGANVLKK42 pKa = 10.7EE43 pKa = 4.32EE44 pKa = 4.45VVSRR48 pKa = 11.84APVRR52 pKa = 11.84RR53 pKa = 11.84GKK55 pKa = 10.28LRR57 pKa = 11.84RR58 pKa = 11.84NVVVLSRR65 pKa = 11.84RR66 pKa = 11.84SRR68 pKa = 11.84DD69 pKa = 3.03GGMEE73 pKa = 4.05SGVHH77 pKa = 4.84IRR79 pKa = 11.84GVNPDD84 pKa = 3.81TGNSDD89 pKa = 3.26NTMKK93 pKa = 10.84ADD95 pKa = 3.51NPRR98 pKa = 11.84NAFYY102 pKa = 10.46WRR104 pKa = 11.84FVEE107 pKa = 4.15MGTVNMPPHH116 pKa = 6.68PFVRR120 pKa = 11.84PAFDD124 pKa = 3.27VRR126 pKa = 11.84SEE128 pKa = 3.89QAAQVAIARR137 pKa = 11.84MNRR140 pKa = 11.84AIDD143 pKa = 3.53EE144 pKa = 4.28VLRR147 pKa = 11.84RR148 pKa = 3.79

Molecular weight: 16.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

75

0

75

16116

44

1184

214.9

23.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.574 ± 0.515

1.353 ± 0.16

5.585 ± 0.197

6.608 ± 0.303

3.245 ± 0.163

7.179 ± 0.358

1.967 ± 0.151

5.355 ± 0.248

5.287 ± 0.258

8.197 ± 0.269

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.755 ± 0.172

4.219 ± 0.205

4.151 ± 0.281

4.337 ± 0.269

6.714 ± 0.286

6.943 ± 0.237

5.684 ± 0.326

6.515 ± 0.238

1.651 ± 0.138

2.681 ± 0.146
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski