Cellulophaga phage phi13:2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.47

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 127 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S0A5R6|S0A5R6_9CAUD Uncharacterized protein OS=Cellulophaga phage phi13:2 OX=1328030 GN=Phi13:2_gp112 PE=4 SV=1
MM1 pKa = 6.53TTATTSTATKK11 pKa = 10.51GRR13 pKa = 11.84IFLTDD18 pKa = 3.23YY19 pKa = 11.34ASYY22 pKa = 11.66NNGTQFEE29 pKa = 4.23FGHH32 pKa = 5.74WVEE35 pKa = 4.96LDD37 pKa = 3.37QFSDD41 pKa = 3.7AEE43 pKa = 4.16EE44 pKa = 4.09LNEE47 pKa = 4.09YY48 pKa = 9.66ILNHH52 pKa = 5.9FQEE55 pKa = 5.43CDD57 pKa = 3.2EE58 pKa = 4.78KK59 pKa = 11.57SPLDD63 pKa = 3.71SPRR66 pKa = 11.84EE67 pKa = 3.86EE68 pKa = 4.09TMITDD73 pKa = 4.77FEE75 pKa = 4.82DD76 pKa = 4.23FPKK79 pKa = 10.81AFYY82 pKa = 10.45SEE84 pKa = 4.72SMDD87 pKa = 3.58FEE89 pKa = 4.57KK90 pKa = 10.89LFEE93 pKa = 4.29YY94 pKa = 10.45FDD96 pKa = 4.55RR97 pKa = 11.84LDD99 pKa = 3.4NCFYY103 pKa = 11.12DD104 pKa = 3.95ADD106 pKa = 3.7IVEE109 pKa = 4.93AFAEE113 pKa = 4.28LGSYY117 pKa = 10.07DD118 pKa = 4.01INDD121 pKa = 3.06IDD123 pKa = 4.0EE124 pKa = 4.96FFDD127 pKa = 3.9ALEE130 pKa = 4.56DD131 pKa = 4.06SYY133 pKa = 11.89SGQYY137 pKa = 10.8NSDD140 pKa = 3.12EE141 pKa = 4.67DD142 pKa = 3.84FAEE145 pKa = 4.74DD146 pKa = 3.58LAEE149 pKa = 3.98QTGVEE154 pKa = 4.5ISNSWPHH161 pKa = 6.3NCIDD165 pKa = 3.38WEE167 pKa = 4.22RR168 pKa = 11.84AARR171 pKa = 11.84DD172 pKa = 3.39LMYY175 pKa = 10.41DD176 pKa = 3.45YY177 pKa = 11.37SEE179 pKa = 5.06SNGHH183 pKa = 5.47YY184 pKa = 9.81FRR186 pKa = 11.84SII188 pKa = 3.05

Molecular weight: 22.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S0A2N7|S0A2N7_9CAUD Uncharacterized protein OS=Cellulophaga phage phi13:2 OX=1328030 GN=Phi13:2_gp061 PE=4 SV=1
MM1 pKa = 7.02LTNMSMNFAPLATPINVGPTSLRR24 pKa = 11.84MAKK27 pKa = 9.15VHH29 pKa = 7.14IINGYY34 pKa = 8.38IKK36 pKa = 10.06KK37 pKa = 10.23KK38 pKa = 9.47MSIRR42 pKa = 11.84YY43 pKa = 8.66HH44 pKa = 5.83LFLWLFF50 pKa = 3.9

Molecular weight: 5.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

127

0

127

22511

33

1990

177.3

20.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.064 ± 0.243

0.96 ± 0.137

6.512 ± 0.188

8.045 ± 0.376

4.389 ± 0.195

5.682 ± 0.265

1.342 ± 0.159

7.898 ± 0.247

9.267 ± 0.451

8.098 ± 0.243

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.279 ± 0.176

6.872 ± 0.277

2.648 ± 0.127

3.158 ± 0.204

3.598 ± 0.208

6.983 ± 0.243

6.441 ± 0.35

5.646 ± 0.18

1.088 ± 0.091

4.029 ± 0.153
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski