Candidatus Nitrospira nitrificans

Taxonomy: cellular organisms; Bacteria; Nitrospirae; Nitrospira; Nitrospirales; Nitrospiraceae; Nitrospira

Average proteome isoelectric point is 7.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4434 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S4LGJ7|A0A0S4LGJ7_9BACT Uncharacterized protein OS=Candidatus Nitrospira nitrificans OX=1742973 GN=COMA2_20099 PE=4 SV=1
MM1 pKa = 7.69DD2 pKa = 4.86RR3 pKa = 11.84SMVATAWEE11 pKa = 4.02QHH13 pKa = 6.09CAIGWPQFASPHH25 pKa = 5.57QGQLMTIDD33 pKa = 3.98TVISGCVVYY42 pKa = 11.01YY43 pKa = 10.71LDD45 pKa = 5.34SSDD48 pKa = 4.59GLDD51 pKa = 3.63DD52 pKa = 3.53QRR54 pKa = 11.84VAIVKK59 pKa = 10.21DD60 pKa = 3.53CLGDD64 pKa = 3.81LDD66 pKa = 5.0EE67 pKa = 4.91LTEE70 pKa = 4.16TLDD73 pKa = 4.11TEE75 pKa = 4.22SQTYY79 pKa = 8.35FFRR82 pKa = 11.84LRR84 pKa = 11.84EE85 pKa = 4.2LGAMLLGDD93 pKa = 4.38EE94 pKa = 4.72PLSS97 pKa = 3.8

Molecular weight: 10.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S4L703|A0A0S4L703_9BACT Putative Glutamate-ammonia-ligase adenylyltransferase subunit A OS=Candidatus Nitrospira nitrificans OX=1742973 GN=COMA2_100082 PE=4 SV=1
MM1 pKa = 6.96TARR4 pKa = 11.84AADD7 pKa = 3.63NSQLEE12 pKa = 4.51GNCAVLIGTLSVCPSTRR29 pKa = 11.84VCRR32 pKa = 11.84SSSRR36 pKa = 11.84MAAPRR41 pKa = 11.84PSRR44 pKa = 11.84NRR46 pKa = 11.84SASGRR51 pKa = 11.84TAASPLLKK59 pKa = 10.35RR60 pKa = 11.84SWSGMTITISPFTCSTMRR78 pKa = 11.84TRR80 pKa = 11.84DD81 pKa = 3.07ICGTPLMTCTRR92 pKa = 11.84LRR94 pKa = 11.84SRR96 pKa = 11.84RR97 pKa = 11.84MATSLLPGVFGAVSAGLLALRR118 pKa = 11.84LNAEE122 pKa = 3.98GDD124 pKa = 3.6EE125 pKa = 4.53GGVTIGLRR133 pKa = 11.84AALIDD138 pKa = 4.05SLTVRR143 pKa = 11.84YY144 pKa = 9.05FPTLTEE150 pKa = 4.33EE151 pKa = 3.95IEE153 pKa = 4.44YY154 pKa = 8.81ITTKK158 pKa = 10.4NANNKK163 pKa = 7.75VMKK166 pKa = 9.99SAYY169 pKa = 6.57EE170 pKa = 3.87TSHH173 pKa = 7.02RR174 pKa = 11.84SS175 pKa = 3.12

Molecular weight: 18.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4434

0

4434

1215056

20

3275

274.0

30.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.501 ± 0.039

1.066 ± 0.016

5.197 ± 0.03

6.063 ± 0.038

3.604 ± 0.025

7.774 ± 0.049

2.466 ± 0.02

5.306 ± 0.028

4.168 ± 0.042

10.388 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.463 ± 0.021

2.731 ± 0.024

5.064 ± 0.03

3.934 ± 0.025

7.101 ± 0.044

6.023 ± 0.03

5.698 ± 0.041

7.601 ± 0.028

1.345 ± 0.019

2.508 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski