Phalaenopsis equestris amalgavirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Amalgaviridae; unclassified Amalgaviridae

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A3G9M863|A0A3G9M863_9VIRU ORF1+2p OS=Phalaenopsis equestris amalgavirus 1 OX=2069327 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.6SGPSQRR7 pKa = 11.84VVHH10 pKa = 6.16FPGGRR15 pKa = 11.84ADD17 pKa = 3.57PTVEE21 pKa = 4.05LVEE24 pKa = 4.27SLARR28 pKa = 11.84YY29 pKa = 8.91ANEE32 pKa = 3.81GFRR35 pKa = 11.84VHH37 pKa = 5.8TWTPGILARR46 pKa = 11.84SFIPVKK52 pKa = 10.57RR53 pKa = 11.84FIDD56 pKa = 3.64AVRR59 pKa = 11.84VLNNEE64 pKa = 3.6PDD66 pKa = 3.27EE67 pKa = 4.47VVFNRR72 pKa = 11.84LLALGVRR79 pKa = 11.84DD80 pKa = 4.1GWWEE84 pKa = 3.97TTNTCTVSQFYY95 pKa = 10.61KK96 pKa = 9.81FCNWLKK102 pKa = 10.34SAEE105 pKa = 4.09GTTRR109 pKa = 11.84LNEE112 pKa = 3.65IRR114 pKa = 11.84KK115 pKa = 9.16ARR117 pKa = 11.84HH118 pKa = 5.69LEE120 pKa = 4.02KK121 pKa = 10.36KK122 pKa = 9.42AQPGQTLEE130 pKa = 4.18DD131 pKa = 3.66VSLVAALDD139 pKa = 3.71EE140 pKa = 4.34QVAEE144 pKa = 4.15FQQRR148 pKa = 11.84KK149 pKa = 9.34KK150 pKa = 7.62EE151 pKa = 4.04TRR153 pKa = 11.84LEE155 pKa = 4.16FEE157 pKa = 4.73SKK159 pKa = 10.8LIDD162 pKa = 3.78LRR164 pKa = 11.84RR165 pKa = 11.84QIALVQQEE173 pKa = 4.2MSATMKK179 pKa = 10.88AHH181 pKa = 7.26DD182 pKa = 5.01ADD184 pKa = 4.94FSPASIYY191 pKa = 11.03EE192 pKa = 4.01PMDD195 pKa = 4.03DD196 pKa = 4.82LEE198 pKa = 5.15FGEE201 pKa = 5.14ACWNLYY207 pKa = 9.8RR208 pKa = 11.84AEE210 pKa = 4.2CARR213 pKa = 11.84LNQDD217 pKa = 4.0EE218 pKa = 4.98APLDD222 pKa = 4.4DD223 pKa = 5.18GLLEE227 pKa = 4.38AVKK230 pKa = 9.06LTHH233 pKa = 6.73GNAALAMHH241 pKa = 7.49KK242 pKa = 10.95ANFLRR247 pKa = 11.84VGFNRR252 pKa = 11.84NNLKK256 pKa = 10.05HH257 pKa = 6.11WIEE260 pKa = 4.01EE261 pKa = 4.41KK262 pKa = 10.41ILEE265 pKa = 4.7LDD267 pKa = 3.63TVGEE271 pKa = 4.13ARR273 pKa = 11.84RR274 pKa = 11.84ATTFRR279 pKa = 11.84SYY281 pKa = 10.48MATTGGALADD291 pKa = 3.78EE292 pKa = 4.95VRR294 pKa = 11.84AEE296 pKa = 4.12TQTRR300 pKa = 11.84VAAAGGTRR308 pKa = 11.84RR309 pKa = 11.84TLRR312 pKa = 11.84RR313 pKa = 11.84ARR315 pKa = 11.84DD316 pKa = 3.41QVDD319 pKa = 3.59VEE321 pKa = 4.36NVQPSRR327 pKa = 11.84LRR329 pKa = 11.84PRR331 pKa = 11.84VTTRR335 pKa = 11.84DD336 pKa = 3.35TGVEE340 pKa = 3.88TAGALVIRR348 pKa = 11.84DD349 pKa = 3.87RR350 pKa = 11.84ASSSHH355 pKa = 5.53TAPPLAEE362 pKa = 4.53PEE364 pKa = 4.31AVHH367 pKa = 6.53PPPAAPGPGGEE378 pKa = 4.53DD379 pKa = 3.49PPLEE383 pKa = 4.19EE384 pKa = 4.71

Molecular weight:
42.87 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A3G9M863|A0A3G9M863_9VIRU ORF1+2p OS=Phalaenopsis equestris amalgavirus 1 OX=2069327 GN=ORF1+ORF2 PE=4 SV=1
MM1 pKa = 7.6SGPSQRR7 pKa = 11.84VVHH10 pKa = 6.16FPGGRR15 pKa = 11.84ADD17 pKa = 3.57PTVEE21 pKa = 4.05LVEE24 pKa = 4.27SLARR28 pKa = 11.84YY29 pKa = 8.91ANEE32 pKa = 3.81GFRR35 pKa = 11.84VHH37 pKa = 5.8TWTPGILARR46 pKa = 11.84SFIPVKK52 pKa = 10.57RR53 pKa = 11.84FIDD56 pKa = 3.64AVRR59 pKa = 11.84VLNNEE64 pKa = 3.6PDD66 pKa = 3.27EE67 pKa = 4.47VVFNRR72 pKa = 11.84LLALGVRR79 pKa = 11.84DD80 pKa = 4.1GWWEE84 pKa = 3.97TTNTCTVSQFYY95 pKa = 10.61KK96 pKa = 9.81FCNWLKK102 pKa = 10.34SAEE105 pKa = 4.09GTTRR109 pKa = 11.84LNEE112 pKa = 3.65IRR114 pKa = 11.84KK115 pKa = 9.16ARR117 pKa = 11.84HH118 pKa = 5.69LEE120 pKa = 4.02KK121 pKa = 10.36KK122 pKa = 9.42AQPGQTLEE130 pKa = 4.18DD131 pKa = 3.66VSLVAALDD139 pKa = 3.71EE140 pKa = 4.34QVAEE144 pKa = 4.15FQQRR148 pKa = 11.84KK149 pKa = 9.34KK150 pKa = 7.62EE151 pKa = 4.04TRR153 pKa = 11.84LEE155 pKa = 4.16FEE157 pKa = 4.73SKK159 pKa = 10.8LIDD162 pKa = 3.78LRR164 pKa = 11.84RR165 pKa = 11.84QIALVQQEE173 pKa = 4.2MSATMKK179 pKa = 10.88AHH181 pKa = 7.26DD182 pKa = 5.01ADD184 pKa = 4.94FSPASIYY191 pKa = 11.03EE192 pKa = 4.01PMDD195 pKa = 4.03DD196 pKa = 4.82LEE198 pKa = 5.15FGEE201 pKa = 5.14ACWNLYY207 pKa = 9.8RR208 pKa = 11.84AEE210 pKa = 4.2CARR213 pKa = 11.84LNQDD217 pKa = 4.0EE218 pKa = 4.98APLDD222 pKa = 4.4DD223 pKa = 5.18GLLEE227 pKa = 4.38AVKK230 pKa = 9.06LTHH233 pKa = 6.73GNAALAMHH241 pKa = 7.49KK242 pKa = 10.95ANFLRR247 pKa = 11.84VGFNRR252 pKa = 11.84NNLKK256 pKa = 10.05HH257 pKa = 6.11WIEE260 pKa = 4.01EE261 pKa = 4.41KK262 pKa = 10.41ILEE265 pKa = 4.7LDD267 pKa = 3.63TVGEE271 pKa = 4.13ARR273 pKa = 11.84RR274 pKa = 11.84ATTFVPTWQQRR285 pKa = 11.84VEE287 pKa = 3.6RR288 pKa = 11.84WLMKK292 pKa = 10.54FEE294 pKa = 4.6LKK296 pKa = 10.64LRR298 pKa = 11.84LEE300 pKa = 4.48LLQQVVLGVPCAEE313 pKa = 4.46PGTRR317 pKa = 11.84LTLRR321 pKa = 11.84MSNLLDD327 pKa = 3.88FGLVLRR333 pKa = 11.84RR334 pKa = 11.84EE335 pKa = 4.36ILGSKK340 pKa = 9.69QPAPLSSEE348 pKa = 3.66IEE350 pKa = 4.14RR351 pKa = 11.84AVLIPPLLWQNQRR364 pKa = 11.84LSILRR369 pKa = 11.84PLRR372 pKa = 11.84PDD374 pKa = 3.06LGGRR378 pKa = 11.84IPHH381 pKa = 5.7SRR383 pKa = 11.84SRR385 pKa = 11.84YY386 pKa = 5.37EE387 pKa = 3.63AKK389 pKa = 9.73VRR391 pKa = 11.84KK392 pKa = 9.73VIGGGEE398 pKa = 3.96MRR400 pKa = 11.84DD401 pKa = 3.0WRR403 pKa = 11.84AANAMYY409 pKa = 10.46RR410 pKa = 11.84GGGSFSDD417 pKa = 3.71ALKK420 pKa = 10.95LLIDD424 pKa = 4.15ARR426 pKa = 11.84EE427 pKa = 4.17DD428 pKa = 3.36APGAILSEE436 pKa = 4.03KK437 pKa = 9.65WKK439 pKa = 10.02VDD441 pKa = 3.18SARR444 pKa = 11.84RR445 pKa = 11.84YY446 pKa = 10.21LLLPCGLPVPRR457 pKa = 11.84GPEE460 pKa = 3.57ATKK463 pKa = 10.21MKK465 pKa = 10.54NFNDD469 pKa = 3.98DD470 pKa = 3.2ATAGPALRR478 pKa = 11.84AFGILRR484 pKa = 11.84KK485 pKa = 9.96SGLKK489 pKa = 9.53TSLEE493 pKa = 3.93EE494 pKa = 4.43FAWNCLDD501 pKa = 3.2AFARR505 pKa = 11.84GGAAEE510 pKa = 4.4DD511 pKa = 3.97CLPFVAARR519 pKa = 11.84VGYY522 pKa = 7.78RR523 pKa = 11.84TKK525 pKa = 11.17LLTLSDD531 pKa = 3.67AWSKK535 pKa = 11.14INSCKK540 pKa = 10.1PLGRR544 pKa = 11.84CVMMLDD550 pKa = 3.2AHH552 pKa = 5.87EE553 pKa = 4.36QAFSSPLYY561 pKa = 10.41NVLSNLTHH569 pKa = 7.2LSRR572 pKa = 11.84FQRR575 pKa = 11.84NSGFCNSIVRR585 pKa = 11.84ASSDD589 pKa = 2.63WAMLWKK595 pKa = 10.4DD596 pKa = 3.41VSAASCVVEE605 pKa = 4.82LDD607 pKa = 2.93WKK609 pKa = 10.89KK610 pKa = 10.72FDD612 pKa = 4.24RR613 pKa = 11.84EE614 pKa = 4.03RR615 pKa = 11.84PADD618 pKa = 4.95DD619 pKa = 3.34ISFMIDD625 pKa = 3.13VIISCFEE632 pKa = 4.0AKK634 pKa = 10.07DD635 pKa = 3.78DD636 pKa = 3.99YY637 pKa = 11.47EE638 pKa = 4.11EE639 pKa = 4.6RR640 pKa = 11.84LLLGYY645 pKa = 10.55RR646 pKa = 11.84IMLNRR651 pKa = 11.84ALIEE655 pKa = 4.2RR656 pKa = 11.84CFVTDD661 pKa = 3.67DD662 pKa = 3.76GGVFHH667 pKa = 7.72IDD669 pKa = 2.91GMVPSGSLWTGWLDD683 pKa = 3.31TALNILYY690 pKa = 9.99IGAALRR696 pKa = 11.84HH697 pKa = 5.45VLPDD701 pKa = 3.57TSQAVAKK708 pKa = 10.53CAGDD712 pKa = 4.04DD713 pKa = 3.86NLTLFYY719 pKa = 10.67TDD721 pKa = 5.42LPDD724 pKa = 5.2ASLLNLKK731 pKa = 10.19KK732 pKa = 10.71YY733 pKa = 9.83LNEE736 pKa = 3.57WFRR739 pKa = 11.84AGIEE743 pKa = 4.09DD744 pKa = 3.91EE745 pKa = 4.96DD746 pKa = 5.19FIIHH750 pKa = 6.73RR751 pKa = 11.84PPYY754 pKa = 10.23HH755 pKa = 5.77ITRR758 pKa = 11.84FQATFPPGTDD768 pKa = 3.12LSKK771 pKa = 10.61GTSHH775 pKa = 7.51LLDD778 pKa = 3.45SAKK781 pKa = 9.85WIQIHH786 pKa = 6.74GIMNIDD792 pKa = 3.4EE793 pKa = 4.53AAGLSHH799 pKa = 6.66RR800 pKa = 11.84WKK802 pKa = 10.74YY803 pKa = 11.22SFAGKK808 pKa = 9.41PKK810 pKa = 10.27FLSCYY815 pKa = 8.77WEE817 pKa = 4.29EE818 pKa = 3.98NGNPIRR824 pKa = 11.84PAHH827 pKa = 6.0VNLEE831 pKa = 4.01KK832 pKa = 10.97LLWPEE837 pKa = 5.32GIHH840 pKa = 6.05KK841 pKa = 9.4TIDD844 pKa = 3.47DD845 pKa = 3.85YY846 pKa = 11.81LAAVISMVVDD856 pKa = 3.37NPFNHH861 pKa = 6.68HH862 pKa = 5.94NVNHH866 pKa = 5.55MMHH869 pKa = 7.34RR870 pKa = 11.84FCIIQQVKK878 pKa = 8.77RR879 pKa = 11.84LSVAGIRR886 pKa = 11.84DD887 pKa = 3.68DD888 pKa = 5.43HH889 pKa = 7.41ILSLAHH895 pKa = 6.37IRR897 pKa = 11.84GKK899 pKa = 10.05EE900 pKa = 3.95GEE902 pKa = 4.4MVPFPMVAEE911 pKa = 4.29WRR913 pKa = 11.84RR914 pKa = 11.84TQGWVDD920 pKa = 3.95MEE922 pKa = 4.57SMPYY926 pKa = 8.98LKK928 pKa = 10.29KK929 pKa = 10.49YY930 pKa = 10.58ISDD933 pKa = 3.66FRR935 pKa = 11.84HH936 pKa = 5.63FAAGVMSLYY945 pKa = 10.64ARR947 pKa = 11.84QPGGGIDD954 pKa = 3.0AWRR957 pKa = 11.84FMDD960 pKa = 4.28MIRR963 pKa = 11.84GNVLLGGEE971 pKa = 4.05QFGNDD976 pKa = 2.81FRR978 pKa = 11.84DD979 pKa = 3.6WIKK982 pKa = 10.53FLQNNPLTSYY992 pKa = 9.4LKK994 pKa = 9.71PMRR997 pKa = 11.84RR998 pKa = 11.84FRR1000 pKa = 11.84PQKK1003 pKa = 9.56AARR1006 pKa = 11.84EE1007 pKa = 4.17STEE1010 pKa = 4.92DD1011 pKa = 3.37IISKK1015 pKa = 10.0FKK1017 pKa = 11.08SFSACCGIGDD1027 pKa = 4.01GRR1029 pKa = 11.84TPFASTEE1036 pKa = 4.4SYY1038 pKa = 10.99GSWIADD1044 pKa = 3.4LVPRR1048 pKa = 11.84MQHH1051 pKa = 5.1GKK1053 pKa = 10.62APLATMM1059 pKa = 5.09

Molecular weight:
120.14 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1443

384

1059

721.5

81.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.425 ± 0.911

1.594 ± 0.248

5.96 ± 0.22

6.861 ± 1.01

4.435 ± 0.354

6.445 ± 0.204

2.495 ± 0.068

4.435 ± 0.821

4.643 ± 0.447

10.395 ± 0.574

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.495 ± 0.418

3.95 ± 0.02

5.128 ± 0.269

3.119 ± 0.236

8.177 ± 0.537

5.475 ± 0.586

4.782 ± 1.008

5.891 ± 0.628

2.356 ± 0.356

1.94 ± 0.286

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski