gamma proteobacterium SS-5

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; unclassified Gammaproteobacteria

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3199 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5Q0ELG3|A0A5Q0ELG3_9GAMM Ribosomal RNA small subunit methyltransferase D OS=gamma proteobacterium SS-5 OX=947516 GN=rsmD PE=3 SV=1
MM1 pKa = 7.11SHH3 pKa = 6.88HH4 pKa = 6.86LAAEE8 pKa = 4.12LVFTYY13 pKa = 10.66QEE15 pKa = 4.03VLDD18 pKa = 4.31ALQAGFGQLSNTPVGTSFSTVEE40 pKa = 3.66LLANGFSITLDD51 pKa = 3.56GSFVWNGTVPEE62 pKa = 4.66GSLKK66 pKa = 10.59RR67 pKa = 11.84LFVSHH72 pKa = 6.44EE73 pKa = 3.76RR74 pKa = 11.84DD75 pKa = 3.12AAALEE80 pKa = 4.3GQISISDD87 pKa = 3.85SATLLLTGIASSFAWGDD104 pKa = 3.32YY105 pKa = 10.91DD106 pKa = 3.75ATTTEE111 pKa = 4.14FSNVKK116 pKa = 8.55TVSGFSLDD124 pKa = 3.58MAQVASIGFEE134 pKa = 4.13LLMEE138 pKa = 5.22ANTAVTATDD147 pKa = 3.78GDD149 pKa = 4.09DD150 pKa = 3.55TFDD153 pKa = 3.46GSGAADD159 pKa = 3.38VVYY162 pKa = 10.66SGAGSDD168 pKa = 3.32ILRR171 pKa = 11.84GGDD174 pKa = 3.88GNDD177 pKa = 3.0ILYY180 pKa = 10.97GEE182 pKa = 4.99AGNDD186 pKa = 3.27FLYY189 pKa = 10.65GGRR192 pKa = 11.84GNDD195 pKa = 3.27EE196 pKa = 4.73LYY198 pKa = 10.91GGTGADD204 pKa = 3.68YY205 pKa = 10.88MEE207 pKa = 5.42GGSGADD213 pKa = 3.8KK214 pKa = 11.27YY215 pKa = 11.2FIDD218 pKa = 6.09DD219 pKa = 3.46IGDD222 pKa = 3.68QVVEE226 pKa = 4.04TDD228 pKa = 3.86NNPEE232 pKa = 3.95EE233 pKa = 4.54PEE235 pKa = 3.66AAARR239 pKa = 11.84ILLALDD245 pKa = 3.43ISSTIDD251 pKa = 3.1QVFASIDD258 pKa = 3.68YY259 pKa = 11.02ALTAYY264 pKa = 10.43VEE266 pKa = 4.15NLTLTDD272 pKa = 3.39NAVQATGNEE281 pKa = 4.13LDD283 pKa = 4.45NILTGSSANNILTGGAGNDD302 pKa = 4.27TIDD305 pKa = 3.65GGAGVDD311 pKa = 3.02IAVFSGNHH319 pKa = 5.3SSYY322 pKa = 10.87SINGSAGSYY331 pKa = 9.45TLTGPDD337 pKa = 3.46GTDD340 pKa = 2.51TLTNIEE346 pKa = 4.32RR347 pKa = 11.84LQFADD352 pKa = 4.25KK353 pKa = 10.75KK354 pKa = 10.61LAYY357 pKa = 10.18DD358 pKa = 4.63LGATEE363 pKa = 4.1NAGMSLEE370 pKa = 4.47FINVIASSLVNDD382 pKa = 4.03ASTRR386 pKa = 11.84GLILNFFDD394 pKa = 4.77QGHH397 pKa = 6.07SLNSLFQLAVDD408 pKa = 3.98IGLVSTLAGGSSNEE422 pKa = 3.92AVAQLAYY429 pKa = 10.66RR430 pKa = 11.84NLLGTEE436 pKa = 4.14ADD438 pKa = 3.59QATTATLTGYY448 pKa = 10.54VDD450 pKa = 3.54IMGQAGFLAAVAGMEE465 pKa = 4.16INQQAVGLLGLQQAGMEE482 pKa = 4.27YY483 pKa = 10.8LL484 pKa = 3.67

Molecular weight: 50.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5Q0EJS8|A0A5Q0EJS8_9GAMM Bifunctional diguanylate cyclase/phosphodiesterase OS=gamma proteobacterium SS-5 OX=947516 GN=D5125_09210 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.25NGRR28 pKa = 11.84KK29 pKa = 9.25VLSARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.85GRR39 pKa = 11.84KK40 pKa = 8.98RR41 pKa = 11.84LTVV44 pKa = 3.11

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3199

0

3199

1044158

37

7620

326.4

36.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.357 ± 0.071

1.035 ± 0.019

5.6 ± 0.033

6.389 ± 0.047

3.447 ± 0.027

7.827 ± 0.043

2.282 ± 0.02

5.072 ± 0.037

3.447 ± 0.045

12.256 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.325 ± 0.021

2.898 ± 0.03

5.0 ± 0.042

5.524 ± 0.048

7.114 ± 0.044

5.505 ± 0.033

4.063 ± 0.044

6.025 ± 0.033

1.298 ± 0.019

2.534 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski