Pusillimonas sp. (strain T7-7)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Alcaligenaceae; Pusillimonas; unclassified Pusillimonas

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3751 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4GRR9|F4GRR9_PUSST MFS permease OS=Pusillimonas sp. (strain T7-7) OX=1007105 GN=PT7_3460 PE=4 SV=1
MM1 pKa = 7.63ILDD4 pKa = 3.7VVIEE8 pKa = 4.17DD9 pKa = 3.82LQFRR13 pKa = 11.84AYY15 pKa = 10.56VVISEE20 pKa = 4.14PDD22 pKa = 3.13INLVADD28 pKa = 4.59FVPAEE33 pKa = 4.07QFEE36 pKa = 4.4QDD38 pKa = 3.05GDD40 pKa = 4.04VHH42 pKa = 5.5VTAISHH48 pKa = 6.68PDD50 pKa = 3.14EE51 pKa = 4.98SLVQIQDD58 pKa = 3.12MAFNMNLGDD67 pKa = 4.25AAVFLCGNEE76 pKa = 3.6QVYY79 pKa = 9.58RR80 pKa = 11.84HH81 pKa = 5.42TLEE84 pKa = 4.39EE85 pKa = 4.57LGQHH89 pKa = 5.89TASQLNN95 pKa = 3.58

Molecular weight: 10.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4GXS3|F4GXS3_PUSST Organic hydroperoxide resistance protein OS=Pusillimonas sp. (strain T7-7) OX=1007105 GN=PT7_2964 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.17RR14 pKa = 11.84THH16 pKa = 5.95GFRR19 pKa = 11.84IRR21 pKa = 11.84MKK23 pKa = 9.4TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.03GRR39 pKa = 11.84KK40 pKa = 8.03QLAVV44 pKa = 3.26

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3751

0

3751

1182568

37

4293

315.3

34.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.413 ± 0.049

0.909 ± 0.014

5.327 ± 0.033

5.246 ± 0.041

3.531 ± 0.023

8.163 ± 0.063

2.259 ± 0.019

5.311 ± 0.032

3.522 ± 0.039

10.706 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.65 ± 0.022

3.057 ± 0.026

4.856 ± 0.026

4.352 ± 0.026

6.092 ± 0.039

6.016 ± 0.029

5.305 ± 0.033

7.365 ± 0.035

1.405 ± 0.019

2.514 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski