Mycobacterium phage Hawkeye

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Dclasvirinae; Hawkeyevirus; Mycobacterium virus Hawkeye

Average proteome isoelectric point is 6.33

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 104 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X2KT46|X2KT46_9CAUD Uncharacterized protein OS=Mycobacterium phage Hawkeye OX=1458711 GN=74 PE=4 SV=1
MM1 pKa = 7.38PTYY4 pKa = 10.26CVRR7 pKa = 11.84WRR9 pKa = 11.84QEE11 pKa = 3.8GEE13 pKa = 4.16TTIHH17 pKa = 7.27ADD19 pKa = 3.34SQEE22 pKa = 4.18DD23 pKa = 3.69AEE25 pKa = 4.49EE26 pKa = 4.14AVSGIVNEE34 pKa = 3.86WDD36 pKa = 2.78ITEE39 pKa = 4.32RR40 pKa = 11.84TDD42 pKa = 3.81SQTDD46 pKa = 3.37DD47 pKa = 4.1PEE49 pKa = 6.75IIDD52 pKa = 3.85TQLVSWSDD60 pKa = 3.12

Molecular weight: 6.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X2KSZ3|X2KSZ3_9CAUD Terminase large subunit OS=Mycobacterium phage Hawkeye OX=1458711 GN=9 PE=4 SV=1
MM1 pKa = 8.02DD2 pKa = 4.71ARR4 pKa = 11.84PRR6 pKa = 11.84RR7 pKa = 11.84TRR9 pKa = 11.84DD10 pKa = 3.45GCWTWFGFPSVHH22 pKa = 5.3QHH24 pKa = 5.26PFSWKK29 pKa = 8.24VRR31 pKa = 11.84YY32 pKa = 8.64GALIEE37 pKa = 4.12EE38 pKa = 4.69FGMAKK43 pKa = 10.29GSIRR47 pKa = 11.84RR48 pKa = 11.84DD49 pKa = 2.79IAAPTKK55 pKa = 9.92SAPRR59 pKa = 11.84PVTQKK64 pKa = 10.32QVPIPTKK71 pKa = 9.65LHH73 pKa = 5.84KK74 pKa = 10.28PKK76 pKa = 10.58NANN79 pKa = 3.02

Molecular weight: 9.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

104

0

104

21730

26

1623

208.9

23.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.176 ± 0.338

0.943 ± 0.131

6.521 ± 0.238

6.861 ± 0.279

3.369 ± 0.193

8.058 ± 0.441

1.979 ± 0.181

5.407 ± 0.18

5.665 ± 0.291

7.303 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.352 ± 0.12

4.151 ± 0.14

5.09 ± 0.209

3.613 ± 0.156

6.18 ± 0.385

6.171 ± 0.213

5.697 ± 0.265

6.475 ± 0.186

1.878 ± 0.135

3.111 ± 0.178
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski