Halobacterium hubeiense

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Halobacterium

Average proteome isoelectric point is 4.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3340 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U5H185|A0A0U5H185_9EURY Homolog to tryptophan--tRNA ligase OS=Halobacterium hubeiense OX=1407499 GN=HHUB_1697 PE=4 SV=1
MM1 pKa = 7.13TRR3 pKa = 11.84DD4 pKa = 3.2VCVVVPTIRR13 pKa = 11.84EE14 pKa = 4.15YY15 pKa = 11.11EE16 pKa = 4.22CMRR19 pKa = 11.84AYY21 pKa = 9.73FQNARR26 pKa = 11.84DD27 pKa = 3.73HH28 pKa = 6.77GFDD31 pKa = 3.89LDD33 pKa = 3.75RR34 pKa = 11.84LFVVLVTEE42 pKa = 5.02DD43 pKa = 3.48FCDD46 pKa = 3.56TDD48 pKa = 3.74GMRR51 pKa = 11.84EE52 pKa = 3.9MLDD55 pKa = 3.45EE56 pKa = 4.71EE57 pKa = 5.08GVAGAVFDD65 pKa = 5.28GDD67 pKa = 6.02DD68 pKa = 3.28RR69 pKa = 11.84DD70 pKa = 3.44AWFDD74 pKa = 3.72EE75 pKa = 4.24QGLDD79 pKa = 3.83QYY81 pKa = 11.8SHH83 pKa = 7.69LIPAASHH90 pKa = 5.85AQTSFGLLYY99 pKa = 10.59LWANDD104 pKa = 3.23FEE106 pKa = 5.11YY107 pKa = 11.17GVFIDD112 pKa = 6.19DD113 pKa = 3.97DD114 pKa = 4.33TLPHH118 pKa = 7.13DD119 pKa = 3.54EE120 pKa = 4.0WDD122 pKa = 3.68FFGTHH127 pKa = 6.86LDD129 pKa = 3.7NLAHH133 pKa = 6.32EE134 pKa = 5.1GEE136 pKa = 4.39VEE138 pKa = 4.13EE139 pKa = 5.08VSSNEE144 pKa = 3.26QWVNVLYY151 pKa = 10.87QSDD154 pKa = 3.68SDD156 pKa = 4.29LYY158 pKa = 9.99PRR160 pKa = 11.84GYY162 pKa = 9.69PYY164 pKa = 10.2STMDD168 pKa = 3.93EE169 pKa = 4.4EE170 pKa = 5.38IEE172 pKa = 4.17TDD174 pKa = 3.36TTEE177 pKa = 3.98TDD179 pKa = 3.29HH180 pKa = 7.17VVASQGLWTNVPDD193 pKa = 4.12LDD195 pKa = 4.07AVRR198 pKa = 11.84ILMDD202 pKa = 4.92GDD204 pKa = 4.0LQGQAQTRR212 pKa = 11.84TDD214 pKa = 4.06FEE216 pKa = 5.05DD217 pKa = 3.77FDD219 pKa = 5.58RR220 pKa = 11.84DD221 pKa = 3.62FVAAEE226 pKa = 3.96DD227 pKa = 4.08NYY229 pKa = 10.86LTVCSMNLAFRR240 pKa = 11.84RR241 pKa = 11.84EE242 pKa = 4.16VVPAFYY248 pKa = 10.27QFPMDD253 pKa = 4.19DD254 pKa = 3.98NAWEE258 pKa = 4.08VGRR261 pKa = 11.84FDD263 pKa = 6.32DD264 pKa = 3.32IWSGVLLKK272 pKa = 10.61RR273 pKa = 11.84AADD276 pKa = 3.62VVGGEE281 pKa = 4.51IYY283 pKa = 10.83NGAPLCEE290 pKa = 4.47HH291 pKa = 6.51NKK293 pKa = 10.09APRR296 pKa = 11.84STFDD300 pKa = 3.55DD301 pKa = 4.0LANEE305 pKa = 4.35VAGLEE310 pKa = 4.27LNEE313 pKa = 4.58HH314 pKa = 5.91FWKK317 pKa = 10.56EE318 pKa = 3.97VAAVEE323 pKa = 4.1PDD325 pKa = 3.25EE326 pKa = 5.29DD327 pKa = 3.91SYY329 pKa = 11.86EE330 pKa = 4.05AVARR334 pKa = 11.84AVGEE338 pKa = 4.0RR339 pKa = 11.84LVEE342 pKa = 4.07SDD344 pKa = 3.62YY345 pKa = 11.89EE346 pKa = 4.29EE347 pKa = 4.58YY348 pKa = 11.42NNGAFLAEE356 pKa = 4.37CGEE359 pKa = 4.3YY360 pKa = 10.96LLDD363 pKa = 4.52WVDD366 pKa = 4.07CCAEE370 pKa = 4.04LAEE373 pKa = 4.14QRR375 pKa = 11.84AVAVADD381 pKa = 4.02DD382 pKa = 3.65

Molecular weight: 43.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U5AJY2|A0A0U5AJY2_9EURY Uncharacterized protein OS=Halobacterium hubeiense OX=1407499 GN=HHUB_4276 PE=4 SV=1
MM1 pKa = 7.63TEE3 pKa = 4.07YY4 pKa = 10.83QPGACNIGHH13 pKa = 6.43AEE15 pKa = 3.71RR16 pKa = 11.84RR17 pKa = 11.84RR18 pKa = 11.84RR19 pKa = 11.84YY20 pKa = 8.06LTGVAGFAAAALLVAAVATVHH41 pKa = 7.43ADD43 pKa = 4.13RR44 pKa = 11.84AWLLAAVAPLFVGFLGVLQARR65 pKa = 11.84ARR67 pKa = 11.84FCVGFAMAGVFDD79 pKa = 4.36VSASGDD85 pKa = 3.38RR86 pKa = 11.84RR87 pKa = 11.84QPAPEE92 pKa = 4.39AGQRR96 pKa = 11.84ADD98 pKa = 3.53RR99 pKa = 11.84KK100 pKa = 9.4RR101 pKa = 11.84AIVLSVKK108 pKa = 10.27ALAAATVGALALYY121 pKa = 9.65LAAGALAA128 pKa = 4.7

Molecular weight: 13.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3340

0

3340

910421

28

1687

272.6

29.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.596 ± 0.081

0.677 ± 0.015

8.604 ± 0.063

8.541 ± 0.067

3.381 ± 0.029

8.325 ± 0.041

2.019 ± 0.023

3.432 ± 0.039

1.856 ± 0.027

8.849 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.685 ± 0.019

2.368 ± 0.027

4.552 ± 0.029

2.629 ± 0.027

6.312 ± 0.046

5.387 ± 0.034

6.192 ± 0.042

9.677 ± 0.056

1.147 ± 0.017

2.773 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski