Gordonia phage Denise

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 78 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5P8DE52|A0A5P8DE52_9CAUD Uncharacterized protein OS=Gordonia phage Denise OX=2652879 GN=77 PE=4 SV=1
MM1 pKa = 7.51TGIDD5 pKa = 3.28WGGGPNWDD13 pKa = 2.97LWRR16 pKa = 11.84WVMSRR21 pKa = 11.84PNPYY25 pKa = 9.12TGEE28 pKa = 4.29DD29 pKa = 2.64AWQRR33 pKa = 11.84MQRR36 pKa = 11.84EE37 pKa = 4.41AAGEE41 pKa = 3.88PLPRR45 pKa = 11.84LTTPAEE51 pKa = 3.77RR52 pKa = 11.84AAPDD56 pKa = 4.2RR57 pKa = 11.84NPDD60 pKa = 3.59PYY62 pKa = 10.06TGAMFRR68 pKa = 11.84AEE70 pKa = 4.63HH71 pKa = 6.75PGLTPGQTCPSCEE84 pKa = 4.61DD85 pKa = 3.81EE86 pKa = 5.19DD87 pKa = 5.82SSIADD92 pKa = 3.91DD93 pKa = 4.66GVCYY97 pKa = 10.62LCVCGCSYY105 pKa = 11.11CEE107 pKa = 4.64PDD109 pKa = 4.17DD110 pKa = 3.84AA111 pKa = 7.27

Molecular weight: 12.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5P8DCF8|A0A5P8DCF8_9CAUD Membrane protein OS=Gordonia phage Denise OX=2652879 GN=68 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.05PGYY5 pKa = 10.1LDD7 pKa = 3.85RR8 pKa = 11.84KK9 pKa = 10.08LSRR12 pKa = 11.84QFQVAPYY19 pKa = 10.27LSTAKK24 pKa = 9.33WRR26 pKa = 11.84PKK28 pKa = 9.04YY29 pKa = 10.48AKK31 pKa = 9.45SAQRR35 pKa = 11.84TTCDD39 pKa = 2.83EE40 pKa = 3.95RR41 pKa = 11.84FAAQHH46 pKa = 5.73EE47 pKa = 4.76SRR49 pKa = 11.84GQYY52 pKa = 9.99GPRR55 pKa = 11.84ARR57 pKa = 11.84VAHH60 pKa = 6.05TRR62 pKa = 11.84TMPDD66 pKa = 2.73GSKK69 pKa = 10.74LYY71 pKa = 10.63LCSAHH76 pKa = 5.67NQLWRR81 pKa = 11.84DD82 pKa = 3.34LDD84 pKa = 3.72EE85 pKa = 4.15TTKK88 pKa = 10.38PKK90 pKa = 10.12RR91 pKa = 11.84RR92 pKa = 11.84KK93 pKa = 9.31SS94 pKa = 3.37

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

78

0

78

14282

38

1451

183.1

19.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.581 ± 0.43

0.973 ± 0.163

6.995 ± 0.241

6.365 ± 0.316

2.514 ± 0.154

8.57 ± 0.418

2.171 ± 0.221

4.887 ± 0.21

2.906 ± 0.183

7.709 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.171 ± 0.102

2.731 ± 0.164

5.503 ± 0.199

3.55 ± 0.262

7.569 ± 0.294

5.65 ± 0.217

6.631 ± 0.414

7.177 ± 0.323

2.15 ± 0.131

2.199 ± 0.133
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski