Lysobacter sp. Root494

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter; unclassified Lysobacter

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3007 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0Q7PEV1|A0A0Q7PEV1_9GAMM Uncharacterized protein OS=Lysobacter sp. Root494 OX=1736549 GN=ASD14_14350 PE=4 SV=1
MM1 pKa = 7.52PNAVVVNGQPSQPNQNQGGLFQWSFVGAPVGTLANANSPQVQLTPFDD48 pKa = 3.88VAASTQGILRR58 pKa = 11.84LTVTVSGCPGISTKK72 pKa = 10.6DD73 pKa = 3.4VPVTVTNAHH82 pKa = 6.3DD83 pKa = 3.82VVINLPPHH91 pKa = 6.44AVPVASPSTASEE103 pKa = 4.23GMVVTLDD110 pKa = 4.38GSGSWDD116 pKa = 3.19VDD118 pKa = 3.83NPSLTYY124 pKa = 10.76AWLQTVGPAVTLANTGNPAIKK145 pKa = 9.78TFVAPNFADD154 pKa = 3.68DD155 pKa = 3.68TQLTFRR161 pKa = 11.84LTVSDD166 pKa = 3.86GTLSNSDD173 pKa = 3.3TTFVNVSWTNDD184 pKa = 3.15SPVAGLVCPDD194 pKa = 2.99GMFEE198 pKa = 4.21VNEE201 pKa = 4.33GQSVTFDD208 pKa = 3.43ASGSTDD214 pKa = 3.41SDD216 pKa = 3.78DD217 pKa = 5.86GIATYY222 pKa = 10.09EE223 pKa = 3.88WAQLVGLPEE232 pKa = 4.15VTGVADD238 pKa = 3.43WNTAVVTFNAPQLGFGEE255 pKa = 4.46TGLVPFKK262 pKa = 10.71LTVTDD267 pKa = 3.16HH268 pKa = 6.91HH269 pKa = 6.49GAKK272 pKa = 8.77STANCAIFVHH282 pKa = 7.29DD283 pKa = 3.52ITKK286 pKa = 10.0PLLSVPQSPLVAEE299 pKa = 4.36ATSADD304 pKa = 3.89GANVAYY310 pKa = 9.63EE311 pKa = 4.18VSAFDD316 pKa = 5.45AVDD319 pKa = 3.51GDD321 pKa = 4.55LPFPSPYY328 pKa = 10.26LLCEE332 pKa = 4.37PPPGSLFALDD342 pKa = 3.71SLSPVLCSAEE352 pKa = 4.04DD353 pKa = 3.41SAGNGNTATFSVSVVDD369 pKa = 3.82STAPAISVPLSFAVEE384 pKa = 3.95ATGPDD389 pKa = 4.07GAPADD394 pKa = 4.12YY395 pKa = 10.44VAKK398 pKa = 11.02SNDD401 pKa = 3.38AVDD404 pKa = 3.95GEE406 pKa = 4.32RR407 pKa = 11.84DD408 pKa = 4.0AVCMPASGSTFPLNTPGPTTTVHH431 pKa = 7.01CDD433 pKa = 2.76ATDD436 pKa = 3.08AHH438 pKa = 6.57GNEE441 pKa = 3.83ATTRR445 pKa = 11.84TFTVAVHH452 pKa = 6.53DD453 pKa = 4.17TTPPDD458 pKa = 3.08IDD460 pKa = 3.77AATVSADD467 pKa = 3.59LVAEE471 pKa = 4.2ATSPSGASVSFGLPSAHH488 pKa = 7.36DD489 pKa = 4.39LVDD492 pKa = 4.26LDD494 pKa = 4.84AVLVTCAPGSPHH506 pKa = 6.44VFPLGEE512 pKa = 4.11TVVQCDD518 pKa = 3.5AVDD521 pKa = 3.39SRR523 pKa = 11.84GNSTADD529 pKa = 3.27ATPGTSATFKK539 pKa = 9.61VTVRR543 pKa = 11.84DD544 pKa = 3.48TTAPVFGPVSNHH556 pKa = 4.03TVEE559 pKa = 4.37AQSGAGASFAYY570 pKa = 10.09ALPTATDD577 pKa = 3.49VVDD580 pKa = 3.77GDD582 pKa = 3.82RR583 pKa = 11.84TVTCTEE589 pKa = 4.02APALALPGVFPLGSTTVTCSASDD612 pKa = 3.32TRR614 pKa = 11.84GNGGSTSFQVTVVDD628 pKa = 4.15TTAPSLDD635 pKa = 3.46LPANITAEE643 pKa = 4.36ATGSTGAVVNFAVAASDD660 pKa = 4.71AVDD663 pKa = 3.92ADD665 pKa = 3.87VQLVCSSNSGDD676 pKa = 3.4TFALGTTTVQCIGTDD691 pKa = 3.5DD692 pKa = 4.37AGNSAGGNFTITVQDD707 pKa = 3.49TTGPAISAHH716 pKa = 5.08EE717 pKa = 4.28TVTAYY722 pKa = 8.96ATGNSAATVTYY733 pKa = 7.71TVPTAVDD740 pKa = 3.85LVDD743 pKa = 4.15GPVVVTCTPASGSSFNVGTTTVTCSAKK770 pKa = 10.44DD771 pKa = 3.48SRR773 pKa = 11.84NNPSNSTFAVTVTYY787 pKa = 10.99NFTGFFQPIDD797 pKa = 3.88NGTMNSVKK805 pKa = 10.18AGSAIPVKK813 pKa = 10.62FSLGGNQGLNIFDD826 pKa = 5.19PGPASGVIACSATDD840 pKa = 3.33GDD842 pKa = 4.73AIEE845 pKa = 4.39EE846 pKa = 4.37TVTAGNSSLQFDD858 pKa = 4.45PGSNQYY864 pKa = 10.4IYY866 pKa = 10.57VWKK869 pKa = 9.17TEE871 pKa = 4.02KK872 pKa = 10.2SWAGQCRR879 pKa = 11.84VLQVKK884 pKa = 10.09LKK886 pKa = 10.99GGTQRR891 pKa = 11.84SALFKK896 pKa = 10.82FKK898 pKa = 11.01

Molecular weight: 91.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0Q7Q9F8|A0A0Q7Q9F8_9GAMM Acetyl-CoA hydrolase OS=Lysobacter sp. Root494 OX=1736549 GN=ASD14_02550 PE=4 SV=1
MM1 pKa = 7.5AMKK4 pKa = 10.25KK5 pKa = 9.72AAKK8 pKa = 9.53KK9 pKa = 10.4KK10 pKa = 9.39PAAKK14 pKa = 9.97KK15 pKa = 9.35AAKK18 pKa = 9.74KK19 pKa = 9.29VAKK22 pKa = 8.89KK23 pKa = 9.1TGAKK27 pKa = 9.57KK28 pKa = 9.95AAKK31 pKa = 8.47KK32 pKa = 7.26TAKK35 pKa = 10.3KK36 pKa = 10.1AVKK39 pKa = 9.89KK40 pKa = 10.0AAKK43 pKa = 8.55KK44 pKa = 7.07TAKK47 pKa = 10.35KK48 pKa = 8.97ATKK51 pKa = 9.94KK52 pKa = 8.93VAKK55 pKa = 10.26RR56 pKa = 11.84PAKK59 pKa = 10.12KK60 pKa = 9.82AAKK63 pKa = 8.57KK64 pKa = 7.23TAKK67 pKa = 10.12KK68 pKa = 9.86AAKK71 pKa = 8.54KK72 pKa = 6.97TAKK75 pKa = 10.33RR76 pKa = 11.84PAKK79 pKa = 10.07KK80 pKa = 9.8AAKK83 pKa = 8.57KK84 pKa = 7.23TAKK87 pKa = 10.12KK88 pKa = 9.86AAKK91 pKa = 8.54KK92 pKa = 6.97TAKK95 pKa = 10.33RR96 pKa = 11.84PAKK99 pKa = 10.07KK100 pKa = 9.8AAKK103 pKa = 8.57KK104 pKa = 7.23TAKK107 pKa = 10.14KK108 pKa = 9.81AAKK111 pKa = 9.61KK112 pKa = 9.2AAKK115 pKa = 9.96RR116 pKa = 11.84PAKK119 pKa = 10.0KK120 pKa = 10.07ASKK123 pKa = 10.11KK124 pKa = 9.69RR125 pKa = 11.84ASKK128 pKa = 10.53KK129 pKa = 9.51SAAAAMPATPAPMII143 pKa = 4.39

Molecular weight: 14.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3007

0

3007

981173

41

2982

326.3

35.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.682 ± 0.063

0.823 ± 0.013

5.875 ± 0.033

5.629 ± 0.044

3.511 ± 0.028

8.439 ± 0.052

2.29 ± 0.023

4.322 ± 0.028

3.004 ± 0.042

10.436 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.221 ± 0.018

2.685 ± 0.027

5.209 ± 0.031

3.562 ± 0.025

7.637 ± 0.049

5.263 ± 0.029

5.095 ± 0.039

7.462 ± 0.038

1.503 ± 0.02

2.352 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski