Pseudomonas phage phiPMW

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Plaisancevirus; Pseudomonas virus PMW

Average proteome isoelectric point is 5.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 229 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5R1D9|A0A1S5R1D9_9CAUD Ribose-phosphate pyrophosphokinase OS=Pseudomonas phage phiPMW OX=1815582 GN=PMW_107 PE=4 SV=1
MM1 pKa = 7.37NKK3 pKa = 8.89TYY5 pKa = 11.25YY6 pKa = 9.29MAWNPAGNEE15 pKa = 3.66AFMTDD20 pKa = 3.71DD21 pKa = 4.54EE22 pKa = 4.68VDD24 pKa = 3.15AMYY27 pKa = 10.88ASTGEE32 pKa = 4.1EE33 pKa = 4.16PPGGVSSLAYY43 pKa = 9.62EE44 pKa = 3.99FRR46 pKa = 11.84EE47 pKa = 4.77LYY49 pKa = 10.79EE50 pKa = 4.33EE51 pKa = 4.65DD52 pKa = 4.85LGFGDD57 pKa = 5.3DD58 pKa = 4.94FRR60 pKa = 11.84ITTISMPTVEE70 pKa = 5.19EE71 pKa = 3.96IRR73 pKa = 3.94

Molecular weight: 8.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S5R188|A0A1S5R188_9CAUD Uncharacterized protein OS=Pseudomonas phage phiPMW OX=1815582 GN=PMW_49 PE=4 SV=1
MM1 pKa = 7.54SFPSGYY7 pKa = 10.68NKK9 pKa = 10.21FISYY13 pKa = 11.02SKK15 pKa = 9.61MIVYY19 pKa = 10.31NVFSWRR25 pKa = 11.84IMTTSRR31 pKa = 11.84QDD33 pKa = 3.39TQSSQNKK40 pKa = 7.71EE41 pKa = 3.46RR42 pKa = 11.84HH43 pKa = 5.47HH44 pKa = 7.11ASNQRR49 pKa = 11.84HH50 pKa = 4.53PHH52 pKa = 5.58FPRR55 pKa = 11.84LLWHH59 pKa = 5.91CHH61 pKa = 3.72PWYY64 pKa = 10.52LRR66 pKa = 11.84SFPHH70 pKa = 6.78CC71 pKa = 4.09

Molecular weight: 8.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

229

0

229

31933

35

1922

139.4

15.74

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.506 ± 0.393

1.34 ± 0.13

6.345 ± 0.161

7.322 ± 0.254

4.099 ± 0.214

7.152 ± 0.183

2.195 ± 0.177

5.803 ± 0.112

6.323 ± 0.248

7.513 ± 0.192

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.298 ± 0.149

4.726 ± 0.21

3.207 ± 0.173

3.839 ± 0.195

4.691 ± 0.127

5.631 ± 0.166

6.373 ± 0.553

6.952 ± 0.149

1.666 ± 0.097

4.021 ± 0.172
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski