Pectobacterium bacteriophage PM2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 291 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A0Q0Q6|A0A0A0Q0Q6_9CAUD Uncharacterized protein OS=Pectobacterium bacteriophage PM2 OX=1429794 GN=PM2_156 PE=4 SV=1
MM1 pKa = 7.21SVNVEE6 pKa = 4.03CAIRR10 pKa = 11.84TATAALNNLIQIAKK24 pKa = 10.29DD25 pKa = 3.34EE26 pKa = 4.79GEE28 pKa = 4.39SEE30 pKa = 3.94VDD32 pKa = 2.48IWLDD36 pKa = 3.27FDD38 pKa = 4.65YY39 pKa = 11.2DD40 pKa = 4.26GEE42 pKa = 4.53GTKK45 pKa = 10.72CLTLKK50 pKa = 10.07TEE52 pKa = 4.4DD53 pKa = 3.71WASSSYY59 pKa = 11.34SCC61 pKa = 5.51

Molecular weight: 6.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A0Q0P2|A0A0A0Q0P2_9CAUD Endonuclease V OS=Pectobacterium bacteriophage PM2 OX=1429794 GN=PM2_131 PE=4 SV=1
MM1 pKa = 7.6LKK3 pKa = 10.44LIYY6 pKa = 9.91EE7 pKa = 4.56AGSKK11 pKa = 10.84GLMVNTRR18 pKa = 11.84DD19 pKa = 3.28SVQRR23 pKa = 11.84KK24 pKa = 7.73EE25 pKa = 4.03FSEE28 pKa = 4.22LKK30 pKa = 10.15QAGLVKK36 pKa = 10.65ASLGIGNSLRR46 pKa = 11.84VTLTSAGIAKK56 pKa = 8.18FAPKK60 pKa = 9.66RR61 pKa = 11.84RR62 pKa = 11.84KK63 pKa = 9.33

Molecular weight: 6.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

291

0

291

53617

32

1242

184.3

20.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.076 ± 0.18

1.123 ± 0.069

6.14 ± 0.098

7.074 ± 0.183

4.61 ± 0.115

5.791 ± 0.183

1.684 ± 0.066

7.766 ± 0.132

7.746 ± 0.192

7.74 ± 0.124

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.663 ± 0.074

5.953 ± 0.123

3.426 ± 0.082

3.346 ± 0.088

4.135 ± 0.087

7.043 ± 0.15

5.735 ± 0.175

6.239 ± 0.13

1.399 ± 0.054

4.31 ± 0.127
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski