Big Cypress virus

Taxonomy: Viruses; unclassified viruses

Average proteome isoelectric point is 7.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1U7EIT7|A0A1U7EIT7_9VIRU Uncharacterized protein OS=Big Cypress virus OX=1955196 PE=4 SV=1
MM1 pKa = 7.8EE2 pKa = 4.46FTKK5 pKa = 10.45TSQDD9 pKa = 3.28FLLQSLLCVSAILSIPFFTLNTLTEE34 pKa = 4.1LTTRR38 pKa = 11.84SLSTIISINLMINHH52 pKa = 7.19ISLIMNFFLLFCLSAATASVLRR74 pKa = 11.84NEE76 pKa = 5.56LINIIKK82 pKa = 10.5ARR84 pKa = 11.84DD85 pKa = 3.68VPDD88 pKa = 4.64GLPKK92 pKa = 10.54SQIRR96 pKa = 11.84TNLRR100 pKa = 11.84IMRR103 pKa = 11.84AFPNFILSNEE113 pKa = 4.21QTVHH117 pKa = 6.95DD118 pKa = 4.7SPVTFDD124 pKa = 3.92KK125 pKa = 11.12EE126 pKa = 3.98CLEE129 pKa = 4.06AHH131 pKa = 5.81STQSFLFVGCNLPKK145 pKa = 9.78HH146 pKa = 6.22CKK148 pKa = 9.93NVVTHH153 pKa = 6.79TSDD156 pKa = 2.97HH157 pKa = 6.31SLLGFEE163 pKa = 5.27SVYY166 pKa = 10.76CLSFHH171 pKa = 6.79QSNDD175 pKa = 2.92SKK177 pKa = 11.12QISLFDD183 pKa = 3.82LSFEE187 pKa = 3.99KK188 pKa = 10.57RR189 pKa = 11.84DD190 pKa = 3.4IKK192 pKa = 10.24FTAEE196 pKa = 3.65FDD198 pKa = 3.79FQPRR202 pKa = 11.84TTNILKK208 pKa = 10.22NAIPVSFFEE217 pKa = 4.45NFFIAKK223 pKa = 8.43TEE225 pKa = 3.92GRR227 pKa = 11.84FYY229 pKa = 11.25LLPRR233 pKa = 11.84VCRR236 pKa = 11.84EE237 pKa = 3.54RR238 pKa = 11.84FTSDD242 pKa = 2.24SHH244 pKa = 6.14YY245 pKa = 11.03VPSTFTLKK253 pKa = 9.96TDD255 pKa = 3.74DD256 pKa = 3.67VSFCIAHH263 pKa = 6.64KK264 pKa = 10.39FFSNVDD270 pKa = 3.42CTLDD274 pKa = 3.36VGFVSMYY281 pKa = 11.32DD282 pKa = 3.16LFDD285 pKa = 4.24YY286 pKa = 11.46YY287 pKa = 10.94DD288 pKa = 4.0FPVSYY293 pKa = 10.71DD294 pKa = 3.56GAPGAAYY301 pKa = 10.27SGNNIDD307 pKa = 3.4QTEE310 pKa = 4.25FRR312 pKa = 11.84AEE314 pKa = 3.79QYY316 pKa = 10.16YY317 pKa = 10.34KK318 pKa = 10.66SHH320 pKa = 6.8AADD323 pKa = 4.79FIYY326 pKa = 10.05PVSLRR331 pKa = 11.84KK332 pKa = 10.26GGTYY336 pKa = 10.73LSNTKK341 pKa = 10.13SPYY344 pKa = 7.06PPKK347 pKa = 10.79YY348 pKa = 10.09IVFSKK353 pKa = 11.09NDD355 pKa = 3.16ISSTTVHH362 pKa = 6.46CFPRR366 pKa = 11.84LSHH369 pKa = 6.0YY370 pKa = 10.87KK371 pKa = 9.97PFLSTVLSSFLAEE384 pKa = 4.25IGGLFQKK391 pKa = 10.56IFDD394 pKa = 4.11TLIEE398 pKa = 4.28CSEE401 pKa = 4.49TIAEE405 pKa = 5.22FILKK409 pKa = 10.28LLGHH413 pKa = 5.86VVSKK417 pKa = 9.72FLNALLPYY425 pKa = 9.72LSPRR429 pKa = 11.84IVPPLLAFPVAYY441 pKa = 10.07FCFSDD446 pKa = 4.04VIKK449 pKa = 10.93SVIFSFFVFICVV461 pKa = 3.14

Molecular weight: 52.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1U7EIT7|A0A1U7EIT7_9VIRU Uncharacterized protein OS=Big Cypress virus OX=1955196 PE=4 SV=1
MM1 pKa = 7.81SSNQKK6 pKa = 9.83NSKK9 pKa = 8.99PRR11 pKa = 11.84QRR13 pKa = 11.84KK14 pKa = 6.59QVVYY18 pKa = 8.91KK19 pKa = 9.66TVQTRR24 pKa = 11.84TPAKK28 pKa = 10.02LKK30 pKa = 10.45KK31 pKa = 10.13SPSKK35 pKa = 10.21KK36 pKa = 5.25TTKK39 pKa = 8.35WTIDD43 pKa = 3.22SGFTDD48 pKa = 3.62ILNSFTSSVSKK59 pKa = 10.12PAVLLSIIAISAFVVTHH76 pKa = 6.41SSSFSTSIVGKK87 pKa = 8.83WANDD91 pKa = 3.64HH92 pKa = 6.66KK93 pKa = 10.88NTTIGKK99 pKa = 7.77WVSEE103 pKa = 4.05NDD105 pKa = 3.35EE106 pKa = 4.46KK107 pKa = 11.62LLGLAVFAPAVLNSPNSIRR126 pKa = 11.84VMLILCSFLWIVLIPEE142 pKa = 3.95NSVYY146 pKa = 10.27QYY148 pKa = 11.05CLQSLALHH156 pKa = 6.72TYY158 pKa = 9.88FKK160 pKa = 10.98VRR162 pKa = 11.84NPKK165 pKa = 10.17SRR167 pKa = 11.84FLVIAVVVIAYY178 pKa = 9.21IAGFLVVKK186 pKa = 10.62

Molecular weight: 20.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

2980

186

2333

993.3

113.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.671 ± 0.307

1.946 ± 0.298

5.671 ± 0.727

5.57 ± 1.137

7.349 ± 1.123

3.591 ± 0.39

2.517 ± 0.153

6.577 ± 0.332

6.779 ± 0.652

8.993 ± 0.713

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.611 ± 0.256

6.208 ± 0.704

4.128 ± 0.341

2.617 ± 0.106

4.497 ± 0.529

8.893 ± 1.168

6.678 ± 0.134

6.477 ± 0.837

0.537 ± 0.273

3.691 ± 0.181
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski