Escherichia phage RDN8.1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Kayfunavirus; unclassified Kayfunavirus

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G6AH62|A0A6G6AH62_9CAUD Uncharacterized protein OS=Escherichia phage RDN8.1 OX=2600100 PE=4 SV=1
MM1 pKa = 7.37EE2 pKa = 5.49RR3 pKa = 11.84NANAYY8 pKa = 10.1YY9 pKa = 10.68DD10 pKa = 4.28LLAATVGLFNEE21 pKa = 5.32RR22 pKa = 11.84IQYY25 pKa = 10.6DD26 pKa = 4.53EE27 pKa = 4.08IAEE30 pKa = 4.26GDD32 pKa = 3.99DD33 pKa = 3.79YY34 pKa = 11.88SDD36 pKa = 4.02ALHH39 pKa = 6.32EE40 pKa = 4.48VVDD43 pKa = 4.59GQVPHH48 pKa = 6.81YY49 pKa = 8.66YY50 pKa = 10.32HH51 pKa = 7.62EE52 pKa = 4.81IFTVMAADD60 pKa = 5.56GISHH64 pKa = 6.98EE65 pKa = 4.76FNDD68 pKa = 4.06SGLMPEE74 pKa = 4.54TKK76 pKa = 10.2DD77 pKa = 3.18VTRR80 pKa = 11.84ILQARR85 pKa = 11.84IYY87 pKa = 8.84EE88 pKa = 4.05ALYY91 pKa = 10.8NDD93 pKa = 3.95VSNSSDD99 pKa = 3.74VVWFEE104 pKa = 4.38AEE106 pKa = 4.0EE107 pKa = 4.19SDD109 pKa = 5.07EE110 pKa = 4.95EE111 pKa = 4.11EE112 pKa = 4.14DD113 pKa = 4.17EE114 pKa = 4.52YY115 pKa = 11.13WVVDD119 pKa = 3.63AKK121 pKa = 10.47TGVFIEE127 pKa = 4.3QAVSLDD133 pKa = 3.4VATACAKK140 pKa = 10.26DD141 pKa = 3.51HH142 pKa = 5.72YY143 pKa = 11.23AIGRR147 pKa = 11.84HH148 pKa = 5.6LKK150 pKa = 10.62VEE152 pKa = 4.88DD153 pKa = 3.52INDD156 pKa = 3.44NVVFDD161 pKa = 4.68PEE163 pKa = 4.23EE164 pKa = 4.22DD165 pKa = 3.89CEE167 pKa = 4.16

Molecular weight: 18.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G6AH22|A0A6G6AH22_9CAUD HNH homing endonuclease OS=Escherichia phage RDN8.1 OX=2600100 PE=4 SV=1
MM1 pKa = 7.26MGALLIVIGYY11 pKa = 9.1GLIAYY16 pKa = 8.18VLVRR20 pKa = 11.84DD21 pKa = 3.6INKK24 pKa = 9.28ARR26 pKa = 11.84KK27 pKa = 8.51VYY29 pKa = 9.84KK30 pKa = 10.31FNYY33 pKa = 8.37VRR35 pKa = 11.84LGRR38 pKa = 11.84WTVRR42 pKa = 11.84QPNGRR47 pKa = 11.84FMRR50 pKa = 11.84NLANVWDD57 pKa = 4.02IATLGSKK64 pKa = 10.07LL65 pKa = 3.58

Molecular weight: 7.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

11285

46

1295

230.3

25.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.808 ± 0.432

0.842 ± 0.158

6.256 ± 0.275

6.69 ± 0.347

3.509 ± 0.188

7.878 ± 0.394

1.843 ± 0.258

4.856 ± 0.173

6.478 ± 0.409

7.763 ± 0.303

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.88 ± 0.129

4.315 ± 0.257

3.704 ± 0.21

4.005 ± 0.436

5.574 ± 0.208

6.38 ± 0.32

5.848 ± 0.276

7.444 ± 0.335

1.48 ± 0.161

3.447 ± 0.139
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski