Puniceispirillum phage HMO-2011

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 74 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S4S2M4|S4S2M4_9CAUD Putative tail fiber protein OS=Puniceispirillum phage HMO-2011 OX=948071 GN=phage1322_40 PE=4 SV=1
MM1 pKa = 7.87PYY3 pKa = 9.68IGKK6 pKa = 9.45SPQNGVRR13 pKa = 11.84NRR15 pKa = 11.84FIYY18 pKa = 10.12QATAGQTSFSGSDD31 pKa = 3.23SDD33 pKa = 5.25SKK35 pKa = 11.54VLTYY39 pKa = 10.52QDD41 pKa = 3.89GLYY44 pKa = 9.76MDD46 pKa = 4.73VFQNGVLLKK55 pKa = 10.64PGTDD59 pKa = 3.62YY60 pKa = 10.63TATTGTTVVLVTGASSGDD78 pKa = 3.64VVEE81 pKa = 4.66MVAYY85 pKa = 10.28DD86 pKa = 3.78VFSVANSYY94 pKa = 8.3TVTEE98 pKa = 4.02SDD100 pKa = 2.54TRR102 pKa = 11.84YY103 pKa = 9.72PFKK106 pKa = 10.99GNNSIIRR113 pKa = 11.84LNGQSITSDD122 pKa = 3.24VTIDD126 pKa = 3.35ADD128 pKa = 4.06EE129 pKa = 5.04NGVSAGPITQDD140 pKa = 2.94NATVTVNGYY149 pKa = 8.79WSIVV153 pKa = 3.22

Molecular weight: 16.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S4S2G2|S4S2G2_9CAUD Uncharacterized protein OS=Puniceispirillum phage HMO-2011 OX=948071 GN=phage1322_73 PE=4 SV=1
MM1 pKa = 7.2TRR3 pKa = 11.84INLFALDD10 pKa = 3.66SKK12 pKa = 11.56AYY14 pKa = 9.93LVLGRR19 pKa = 11.84TLSLLKK25 pKa = 9.98IKK27 pKa = 10.25HH28 pKa = 5.21VRR30 pKa = 11.84SPTMHH35 pKa = 7.0VMMHH39 pKa = 6.53RR40 pKa = 11.84GSRR43 pKa = 11.84PASCQRR49 pKa = 11.84STGHH53 pKa = 6.46IKK55 pKa = 11.01LNGNIGDD62 pKa = 3.46ISMGIIKK69 pKa = 8.84TLAKK73 pKa = 10.25GAAKK77 pKa = 10.07KK78 pKa = 10.37ARR80 pKa = 11.84EE81 pKa = 4.05KK82 pKa = 10.78QGEE85 pKa = 3.94AGKK88 pKa = 10.52ARR90 pKa = 11.84RR91 pKa = 11.84AAKK94 pKa = 10.29AKK96 pKa = 10.83AEE98 pKa = 4.07AAKK101 pKa = 10.35KK102 pKa = 9.93AASKK106 pKa = 10.4KK107 pKa = 10.13AASKK111 pKa = 10.55PKK113 pKa = 9.23ATQKK117 pKa = 10.93GLRR120 pKa = 11.84LSRR123 pKa = 11.84KK124 pKa = 8.71PVTQEE129 pKa = 3.97GMGLDD134 pKa = 4.6AITGRR139 pKa = 11.84SNMAAASVDD148 pKa = 3.06KK149 pKa = 11.2GKK151 pKa = 10.57AGKK154 pKa = 7.59VTRR157 pKa = 11.84SAEE160 pKa = 3.95PGFLQSQRR168 pKa = 11.84TAGSRR173 pKa = 11.84AKK175 pKa = 10.47AQEE178 pKa = 4.06KK179 pKa = 10.42VDD181 pKa = 3.94LAKK184 pKa = 10.48KK185 pKa = 10.01VRR187 pKa = 11.84DD188 pKa = 3.81GKK190 pKa = 9.46ATKK193 pKa = 10.37AEE195 pKa = 4.06KK196 pKa = 10.6AKK198 pKa = 10.76LKK200 pKa = 10.41SLRR203 pKa = 11.84EE204 pKa = 3.81KK205 pKa = 10.45DD206 pKa = 3.86AKK208 pKa = 10.07DD209 pKa = 3.23TASARR214 pKa = 11.84AKK216 pKa = 10.2GAASRR221 pKa = 11.84KK222 pKa = 8.77KK223 pKa = 10.09KK224 pKa = 10.76SKK226 pKa = 9.59ATLPEE231 pKa = 4.43LKK233 pKa = 10.05SAPSKK238 pKa = 10.79KK239 pKa = 8.28VTKK242 pKa = 9.79EE243 pKa = 3.65KK244 pKa = 10.8PPINLKK250 pKa = 9.43TGEE253 pKa = 4.1INKK256 pKa = 9.88SVFNKK261 pKa = 10.33LSPGKK266 pKa = 9.96QEE268 pKa = 3.81QAVRR272 pKa = 11.84NAMARR277 pKa = 11.84VEE279 pKa = 4.35GPRR282 pKa = 11.84KK283 pKa = 9.74RR284 pKa = 11.84EE285 pKa = 3.6LKK287 pKa = 10.82AFLEE291 pKa = 4.48EE292 pKa = 4.1MRR294 pKa = 11.84KK295 pKa = 9.64SKK297 pKa = 10.49PGEE300 pKa = 3.79SGLRR304 pKa = 11.84RR305 pKa = 11.84RR306 pKa = 11.84KK307 pKa = 8.31GTRR310 pKa = 11.84GMSGAEE316 pKa = 3.93SSIKK320 pKa = 10.75DD321 pKa = 3.4LDD323 pKa = 3.75KK324 pKa = 11.48GVGGRR329 pKa = 11.84GGLDD333 pKa = 3.43FNRR336 pKa = 11.84GGMVKK341 pKa = 9.91KK342 pKa = 10.38RR343 pKa = 11.84KK344 pKa = 9.29KK345 pKa = 10.24

Molecular weight: 37.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

74

0

74

17041

30

1128

230.3

25.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.536 ± 0.495

0.78 ± 0.113

6.76 ± 0.279

6.109 ± 0.44

3.433 ± 0.208

7.306 ± 0.343

1.491 ± 0.194

5.047 ± 0.179

6.238 ± 0.504

7.153 ± 0.249

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.163 ± 0.232

4.841 ± 0.21

3.767 ± 0.267

4.178 ± 0.35

4.853 ± 0.305

6.754 ± 0.356

7.288 ± 0.497

6.602 ± 0.252

1.262 ± 0.138

3.439 ± 0.174
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski