Pigeonpea sterility mosaic emaravirus 1

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Fimoviridae; Emaravirus

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U6E7B1|U6E7B1_9VIRU p5 OS=Pigeonpea sterility mosaic emaravirus 1 OX=1980429 GN=p5 PE=4 SV=2
MM1 pKa = 7.36EE2 pKa = 6.02SIVPFCISKK11 pKa = 10.71KK12 pKa = 9.11EE13 pKa = 4.01VINNSNYY20 pKa = 10.12EE21 pKa = 3.87KK22 pKa = 10.91LKK24 pKa = 9.91PNKK27 pKa = 9.71NLGRR31 pKa = 11.84MEE33 pKa = 4.72RR34 pKa = 11.84IIYY37 pKa = 10.52DD38 pKa = 3.82MIKK41 pKa = 10.71DD42 pKa = 3.51EE43 pKa = 4.39YY44 pKa = 10.06NRR46 pKa = 11.84CNAVEE51 pKa = 4.7KK52 pKa = 11.13GMFCDD57 pKa = 4.61DD58 pKa = 3.84FNMIPINSCIGYY70 pKa = 8.13MEE72 pKa = 5.28IGDD75 pKa = 5.26DD76 pKa = 3.42IPTYY80 pKa = 10.94VYY82 pKa = 10.52AIKK85 pKa = 10.44KK86 pKa = 8.29GAYY89 pKa = 9.11FKK91 pKa = 11.66GEE93 pKa = 3.63FQYY96 pKa = 11.64AVIEE100 pKa = 4.2LSDD103 pKa = 3.82AVSGAINSILYY114 pKa = 10.01SAVIRR119 pKa = 11.84DD120 pKa = 3.88FEE122 pKa = 6.35GDD124 pKa = 3.33MDD126 pKa = 4.25TLYY129 pKa = 9.5ITVKK133 pKa = 10.18GSLPIKK139 pKa = 9.89EE140 pKa = 3.95YY141 pKa = 10.76FIIVKK146 pKa = 9.89IEE148 pKa = 3.81NKK150 pKa = 7.93QTLIQRR156 pKa = 11.84AKK158 pKa = 10.83SEE160 pKa = 3.9WANILSSTSLVGTDD174 pKa = 4.36SIVSHH179 pKa = 5.41YY180 pKa = 11.13RR181 pKa = 11.84EE182 pKa = 4.01MTRR185 pKa = 11.84FCYY188 pKa = 10.1RR189 pKa = 11.84IIKK192 pKa = 8.65NQSDD196 pKa = 3.76VGSVYY201 pKa = 11.47SMISEE206 pKa = 4.03NQHH209 pKa = 5.66LVNHH213 pKa = 6.19VLPSLTMYY221 pKa = 8.11LTSYY225 pKa = 10.78NSVILEE231 pKa = 4.05HH232 pKa = 6.44VKK234 pKa = 10.48NHH236 pKa = 5.99IKK238 pKa = 10.25RR239 pKa = 11.84CYY241 pKa = 9.85EE242 pKa = 3.95SNDD245 pKa = 3.15YY246 pKa = 11.37NLIFRR251 pKa = 11.84KK252 pKa = 10.11DD253 pKa = 3.93DD254 pKa = 3.53IEE256 pKa = 5.58CDD258 pKa = 3.63HH259 pKa = 6.47LTEE262 pKa = 4.75PYY264 pKa = 9.75IDD266 pKa = 4.1EE267 pKa = 4.32GLKK270 pKa = 10.87LEE272 pKa = 4.46ISNAIYY278 pKa = 10.97NDD280 pKa = 2.9VTLRR284 pKa = 11.84LRR286 pKa = 11.84EE287 pKa = 4.07IKK289 pKa = 10.59NCEE292 pKa = 3.82AEE294 pKa = 3.84AKK296 pKa = 10.03YY297 pKa = 10.3IKK299 pKa = 10.49KK300 pKa = 10.24NLFRR304 pKa = 11.84SDD306 pKa = 3.32NNKK309 pKa = 9.98IISDD313 pKa = 4.01FEE315 pKa = 4.33AVTSSEE321 pKa = 4.14TEE323 pKa = 3.9MIKK326 pKa = 10.41YY327 pKa = 10.52LPGTNEE333 pKa = 3.87AEE335 pKa = 5.28DD336 pKa = 4.01FFQYY340 pKa = 11.06GLPEE344 pKa = 5.02HH345 pKa = 6.85INIARR350 pKa = 11.84THH352 pKa = 6.74LEE354 pKa = 3.3NSMVKK359 pKa = 10.39RR360 pKa = 11.84ITDD363 pKa = 3.21ANMIDD368 pKa = 3.95DD369 pKa = 3.79VDD371 pKa = 4.62YY372 pKa = 11.0IPSYY376 pKa = 11.01PFTTEE381 pKa = 3.49FKK383 pKa = 10.35KK384 pKa = 11.03VVFEE388 pKa = 4.3SYY390 pKa = 10.17RR391 pKa = 11.84IKK393 pKa = 11.12NKK395 pKa = 6.63TVKK398 pKa = 9.43MSHH401 pKa = 6.72LGLHH405 pKa = 5.6YY406 pKa = 10.59ACLYY410 pKa = 9.82IYY412 pKa = 9.96FSIVSTRR419 pKa = 11.84RR420 pKa = 11.84AGYY423 pKa = 9.53EE424 pKa = 3.76RR425 pKa = 11.84FNFDD429 pKa = 2.97HH430 pKa = 7.07KK431 pKa = 11.07LIEE434 pKa = 4.21KK435 pKa = 9.74SRR437 pKa = 11.84KK438 pKa = 6.85FAKK441 pKa = 10.6VNIEE445 pKa = 3.72YY446 pKa = 10.69LRR448 pKa = 11.84FRR450 pKa = 11.84IQTVRR455 pKa = 11.84TVSTPINNCVTITLKK470 pKa = 11.04KK471 pKa = 10.49NLL473 pKa = 3.98

Molecular weight: 55.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U6CPM6|U6CPM6_9VIRU Replicase OS=Pigeonpea sterility mosaic emaravirus 1 OX=1980429 GN=RdRp PE=4 SV=1
MM1 pKa = 7.36PPKK4 pKa = 9.85MPSKK8 pKa = 9.75TPLSNMPAASKK19 pKa = 10.81KK20 pKa = 10.57SDD22 pKa = 3.23IPDD25 pKa = 3.27NEE27 pKa = 3.46VRR29 pKa = 11.84INVKK33 pKa = 10.61GKK35 pKa = 9.9VNTIKK40 pKa = 10.64FPNVKK45 pKa = 9.92DD46 pKa = 3.58KK47 pKa = 11.12KK48 pKa = 9.75LQNTEE53 pKa = 3.99GTSLKK58 pKa = 10.23PATIEE63 pKa = 3.78PKK65 pKa = 10.26AFKK68 pKa = 10.06SAAMVEE74 pKa = 4.08HH75 pKa = 7.18DD76 pKa = 4.75FKK78 pKa = 10.8IKK80 pKa = 10.45KK81 pKa = 9.39YY82 pKa = 10.57ISYY85 pKa = 10.85CNINAAAAYY94 pKa = 9.44LSQSKK99 pKa = 7.37EE100 pKa = 3.86HH101 pKa = 7.78KK102 pKa = 10.54EE103 pKa = 3.56MLKK106 pKa = 10.74QNDD109 pKa = 3.97SLILTVSKK117 pKa = 10.38DD118 pKa = 3.14QKK120 pKa = 10.71IYY122 pKa = 10.7VIQNIQEE129 pKa = 4.23TNIEE133 pKa = 4.1NVLSFNKK140 pKa = 10.08ACAVLALGILKK151 pKa = 10.36HH152 pKa = 6.14KK153 pKa = 10.27FPEE156 pKa = 4.25IFDD159 pKa = 4.09WTSHH163 pKa = 6.16KK164 pKa = 10.75YY165 pKa = 7.65VTSGWSDD172 pKa = 3.2QNLNVEE178 pKa = 4.09DD179 pKa = 4.39TIINRR184 pKa = 11.84LAGAMGLTPDD194 pKa = 3.44NPYY197 pKa = 10.23YY198 pKa = 10.2WFMVPGYY205 pKa = 9.16EE206 pKa = 4.82FLYY209 pKa = 10.17EE210 pKa = 4.63LYY212 pKa = 9.83PAEE215 pKa = 4.83CIAYY219 pKa = 7.55TLLRR223 pKa = 11.84VEE225 pKa = 4.11YY226 pKa = 10.34RR227 pKa = 11.84EE228 pKa = 3.9VLNIPEE234 pKa = 5.0KK235 pKa = 10.56ISNQDD240 pKa = 2.64IVQSLTAKK248 pKa = 9.15MNKK251 pKa = 8.87FHH253 pKa = 6.95GLEE256 pKa = 3.84TSTFKK261 pKa = 11.11DD262 pKa = 3.21AVAVVGLEE270 pKa = 4.1NIKK273 pKa = 10.42AAYY276 pKa = 9.19QAMSSSVGEE285 pKa = 3.97TGRR288 pKa = 11.84TRR290 pKa = 11.84RR291 pKa = 11.84AAIVLAGFEE300 pKa = 4.38EE301 pKa = 4.5LLKK304 pKa = 10.79SLKK307 pKa = 10.1EE308 pKa = 3.89

Molecular weight: 34.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

4059

308

2294

811.8

94.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.523 ± 0.749

1.897 ± 0.436

6.332 ± 0.365

5.79 ± 0.415

4.484 ± 0.272

3.646 ± 0.695

2.488 ± 0.234

8.943 ± 0.632

8.426 ± 0.368

8.795 ± 0.906

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.956 ± 0.145

6.948 ± 0.65

3.277 ± 0.262

2.685 ± 0.328

3.819 ± 0.304

7.982 ± 0.378

6.011 ± 0.349

5.494 ± 0.228

0.616 ± 0.14

5.888 ± 0.503
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski