Mizugakiibacter sediminis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Rhodanobacteraceae; Mizugakiibacter

Average proteome isoelectric point is 7.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2837 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K8QNG9|A0A0K8QNG9_9GAMM GDP-mannose 4 6-dehydratase OS=Mizugakiibacter sediminis OX=1475481 GN=gmd PE=3 SV=1
MM1 pKa = 7.16YY2 pKa = 10.08ALSDD6 pKa = 3.24AHH8 pKa = 5.98VQNVSGGGWGDD19 pKa = 4.07AIGAAVVGWAVGKK32 pKa = 10.66VLDD35 pKa = 4.46AAASAIASQNQGGTAFEE52 pKa = 4.96EE53 pKa = 5.08GDD55 pKa = 3.57STLPANVYY63 pKa = 8.06GTT65 pKa = 3.75

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K8QK18|A0A0K8QK18_9GAMM Phe-4-monooxygenase OS=Mizugakiibacter sediminis OX=1475481 GN=MBSD_1711 PE=3 SV=1
MM1 pKa = 7.76AEE3 pKa = 3.85ALADD7 pKa = 3.79GVARR11 pKa = 11.84FLDD14 pKa = 3.59HH15 pKa = 6.87LAVEE19 pKa = 4.97RR20 pKa = 11.84RR21 pKa = 11.84YY22 pKa = 10.75SPHH25 pKa = 5.91TLAGYY30 pKa = 9.76RR31 pKa = 11.84QDD33 pKa = 3.78LDD35 pKa = 3.71KK36 pKa = 10.97LARR39 pKa = 11.84WMQARR44 pKa = 11.84GLARR48 pKa = 11.84FDD50 pKa = 4.33ALTPDD55 pKa = 4.32LLRR58 pKa = 11.84GFVAAEE64 pKa = 3.86HH65 pKa = 6.49RR66 pKa = 11.84AGLSPKK72 pKa = 9.92SLQRR76 pKa = 11.84VLSTCRR82 pKa = 11.84SLFRR86 pKa = 11.84FLARR90 pKa = 11.84SGALAHH96 pKa = 7.1DD97 pKa = 4.34PAAGVRR103 pKa = 11.84APKK106 pKa = 10.1VRR108 pKa = 11.84RR109 pKa = 11.84KK110 pKa = 10.01LPQVLDD116 pKa = 3.61TDD118 pKa = 3.82EE119 pKa = 5.44ANALVEE125 pKa = 4.41LPAEE129 pKa = 4.52GTLGPRR135 pKa = 11.84DD136 pKa = 3.36RR137 pKa = 11.84AMLEE141 pKa = 4.0LFYY144 pKa = 11.29SSALRR149 pKa = 11.84LSEE152 pKa = 4.68LVGLRR157 pKa = 11.84WGALDD162 pKa = 4.2LDD164 pKa = 4.25EE165 pKa = 5.09GLVRR169 pKa = 11.84VTGKK173 pKa = 10.4GGKK176 pKa = 7.01TRR178 pKa = 11.84IVPVGRR184 pKa = 11.84HH185 pKa = 4.39ACAALRR191 pKa = 11.84ALAAAGGAAPDD202 pKa = 3.56APVFVSRR209 pKa = 11.84HH210 pKa = 4.89GGALTPRR217 pKa = 11.84TVQARR222 pKa = 11.84LKK224 pKa = 9.71TLALRR229 pKa = 11.84QGIAKK234 pKa = 9.97RR235 pKa = 11.84IHH237 pKa = 5.82PHH239 pKa = 6.46LLRR242 pKa = 11.84HH243 pKa = 5.77SCASHH248 pKa = 7.01LLEE251 pKa = 5.06SSGDD255 pKa = 3.51LRR257 pKa = 11.84AVQEE261 pKa = 4.38LLGHH265 pKa = 6.73ADD267 pKa = 2.88IGTTEE272 pKa = 4.59IYY274 pKa = 9.33THH276 pKa = 7.48LDD278 pKa = 3.42FQHH281 pKa = 7.1LARR284 pKa = 11.84VYY286 pKa = 10.55DD287 pKa = 3.9AAHH290 pKa = 5.79PRR292 pKa = 11.84ARR294 pKa = 11.84RR295 pKa = 11.84KK296 pKa = 9.73

Molecular weight: 32.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2837

0

2837

897279

39

1999

316.3

34.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.007 ± 0.096

0.901 ± 0.015

5.65 ± 0.037

5.424 ± 0.047

3.318 ± 0.027

8.526 ± 0.036

2.284 ± 0.023

3.779 ± 0.033

2.569 ± 0.04

11.18 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.009 ± 0.02

2.044 ± 0.028

5.553 ± 0.033

3.211 ± 0.027

8.653 ± 0.054

4.118 ± 0.033

4.52 ± 0.035

7.374 ± 0.037

1.48 ± 0.021

2.401 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski