Amino acid dipepetide frequency for Methanoplanus limicola DSM 2279

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.855AlaAla: 5.855 ± 0.108	0.873AlaCys: 0.873 ± 0.034	4.59AlaAsp: 4.59 ± 0.095	6.294AlaGlu: 6.294 ± 0.095	2.805AlaPhe: 2.805 ± 0.063	7.137AlaGly: 7.137 ± 0.105	0.958AlaHis: 0.958 ± 0.038	5.049AlaIle: 5.049 ± 0.085	3.574AlaLys: 3.574 ± 0.063	6.435AlaLeu: 6.435 ± 0.109	1.802AlaMet: 1.802 ± 0.048	2.006AlaAsn: 2.006 ± 0.058	1.928AlaPro: 1.928 ± 0.057	1.16AlaGln: 1.16 ± 0.037	2.642AlaArg: 2.642 ± 0.063	3.755AlaSer: 3.755 ± 0.081	2.935AlaThr: 2.935 ± 0.071	6.133AlaVal: 6.133 ± 0.092	0.569AlaTrp: 0.569 ± 0.029	2.178AlaTyr: 2.178 ± 0.059	0.0AlaXaa: 0.0 ± 0.0
Cys	0.829CysAla: 0.829 ± 0.031	0.25CysCys: 0.25 ± 0.027	0.771CysAsp: 0.771 ± 0.03	0.816CysGlu: 0.816 ± 0.027	0.437CysPhe: 0.437 ± 0.023	1.491CysGly: 1.491 ± 0.045	0.28CysHis: 0.28 ± 0.018	1.03CysIle: 1.03 ± 0.039	0.547CysLys: 0.547 ± 0.025	1.034CysLeu: 1.034 ± 0.038	0.287CysMet: 0.287 ± 0.017	0.558CysAsn: 0.558 ± 0.025	0.797CysPro: 0.797 ± 0.035	0.239CysGln: 0.239 ± 0.018	0.909CysArg: 0.909 ± 0.035	0.913CysSer: 0.913 ± 0.031	0.79CysThr: 0.79 ± 0.028	0.635CysVal: 0.635 ± 0.03	0.119CysTrp: 0.119 ± 0.013	0.47CysTyr: 0.47 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	3.965AspAla: 3.965 ± 0.077	0.725AspCys: 0.725 ± 0.028	3.547AspAsp: 3.547 ± 0.081	5.34AspGlu: 5.34 ± 0.088	2.743AspPhe: 2.743 ± 0.063	4.285AspGly: 4.285 ± 0.093	0.868AspHis: 0.868 ± 0.029	6.057AspIle: 6.057 ± 0.09	3.39AspLys: 3.39 ± 0.06	5.489AspLeu: 5.489 ± 0.083	1.546AspMet: 1.546 ± 0.047	2.914AspAsn: 2.914 ± 0.074	2.446AspPro: 2.446 ± 0.056	1.041AspGln: 1.041 ± 0.04	2.738AspArg: 2.738 ± 0.058	4.199AspSer: 4.199 ± 0.084	2.74AspThr: 2.74 ± 0.073	3.836AspVal: 3.836 ± 0.071	0.551AspTrp: 0.551 ± 0.029	2.772AspTyr: 2.772 ± 0.054	0.0AspXaa: 0.0 ± 0.0
Glu	4.741GluAla: 4.741 ± 0.083	0.916GluCys: 0.916 ± 0.029	4.425GluAsp: 4.425 ± 0.08	7.016GluGlu: 7.016 ± 0.125	3.052GluPhe: 3.052 ± 0.056	4.715GluGly: 4.715 ± 0.078	1.084GluHis: 1.084 ± 0.036	8.122GluIle: 8.122 ± 0.117	6.501GluLys: 6.501 ± 0.109	6.14GluLeu: 6.14 ± 0.095	2.237GluMet: 2.237 ± 0.045	4.396GluAsn: 4.396 ± 0.076	2.119GluPro: 2.119 ± 0.06	1.509GluGln: 1.509 ± 0.049	3.795GluArg: 3.795 ± 0.073	4.903GluSer: 4.903 ± 0.086	3.455GluThr: 3.455 ± 0.076	4.396GluVal: 4.396 ± 0.081	0.741GluTrp: 0.741 ± 0.03	3.139GluTyr: 3.139 ± 0.06	0.0GluXaa: 0.0 ± 0.0
Phe	2.837PheAla: 2.837 ± 0.064	0.676PheCys: 0.676 ± 0.029	2.475PheAsp: 2.475 ± 0.06	2.966PheGlu: 2.966 ± 0.061	1.943PhePhe: 1.943 ± 0.068	3.185PheGly: 3.185 ± 0.065	0.634PheHis: 0.634 ± 0.03	3.412PheIle: 3.412 ± 0.074	2.031PheLys: 2.031 ± 0.05	3.777PheLeu: 3.777 ± 0.085	1.014PheMet: 1.014 ± 0.039	1.657PheAsn: 1.657 ± 0.047	1.564PhePro: 1.564 ± 0.041	0.783PheGln: 0.783 ± 0.029	2.181PheArg: 2.181 ± 0.055	3.764PheSer: 3.764 ± 0.083	2.382PheThr: 2.382 ± 0.062	2.548PheVal: 2.548 ± 0.058	0.407PheTrp: 0.407 ± 0.021	1.467PheTyr: 1.467 ± 0.049	0.0PheXaa: 0.0 ± 0.0
Gly	4.792GlyAla: 4.792 ± 0.086	1.235GlyCys: 1.235 ± 0.038	4.717GlyAsp: 4.717 ± 0.102	5.361GlyGlu: 5.361 ± 0.078	3.231GlyPhe: 3.231 ± 0.061	5.374GlyGly: 5.374 ± 0.129	1.239GlyHis: 1.239 ± 0.037	7.452GlyIle: 7.452 ± 0.118	4.997GlyLys: 4.997 ± 0.076	5.823GlyLeu: 5.823 ± 0.085	2.099GlyMet: 2.099 ± 0.056	3.364GlyAsn: 3.364 ± 0.076	1.84GlyPro: 1.84 ± 0.047	1.478GlyGln: 1.478 ± 0.045	3.946GlyArg: 3.946 ± 0.072	5.091GlySer: 5.091 ± 0.094	4.097GlyThr: 4.097 ± 0.08	4.747GlyVal: 4.747 ± 0.082	0.811GlyTrp: 0.811 ± 0.03	3.209GlyTyr: 3.209 ± 0.063	0.0GlyXaa: 0.0 ± 0.0
His	1.009HisAla: 1.009 ± 0.038	0.296HisCys: 0.296 ± 0.018	0.893HisAsp: 0.893 ± 0.032	1.066HisGlu: 1.066 ± 0.037	0.657HisPhe: 0.657 ± 0.031	1.085HisGly: 1.085 ± 0.036	0.401HisHis: 0.401 ± 0.02	1.404HisIle: 1.404 ± 0.042	0.86HisLys: 0.86 ± 0.035	1.509HisLeu: 1.509 ± 0.044	0.399HisMet: 0.399 ± 0.022	0.771HisAsn: 0.771 ± 0.03	0.93HisPro: 0.93 ± 0.032	0.398HisGln: 0.398 ± 0.021	0.845HisArg: 0.845 ± 0.034	1.059HisSer: 1.059 ± 0.038	0.853HisThr: 0.853 ± 0.031	0.854HisVal: 0.854 ± 0.03	0.151HisTrp: 0.151 ± 0.012	0.645HisTyr: 0.645 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	6.13IleAla: 6.13 ± 0.092	1.215IleCys: 1.215 ± 0.037	4.596IleAsp: 4.596 ± 0.068	5.742IleGlu: 5.742 ± 0.086	3.722IlePhe: 3.722 ± 0.081	5.08IleGly: 5.08 ± 0.092	1.394IleHis: 1.394 ± 0.041	7.679IleIle: 7.679 ± 0.123	5.05IleLys: 5.05 ± 0.099	7.613IleLeu: 7.613 ± 0.12	2.012IleMet: 2.012 ± 0.053	3.881IleAsn: 3.881 ± 0.084	4.142IlePro: 4.142 ± 0.071	1.819IleGln: 1.819 ± 0.051	4.129IleArg: 4.129 ± 0.075	7.566IleSer: 7.566 ± 0.108	5.084IleThr: 5.084 ± 0.089	4.917IleVal: 4.917 ± 0.089	0.667IleTrp: 0.667 ± 0.026	2.881IleTyr: 2.881 ± 0.055	0.0IleXaa: 0.0 ± 0.0
Lys	3.846LysAla: 3.846 ± 0.075	0.725LysCys: 0.725 ± 0.03	3.891LysAsp: 3.891 ± 0.074	5.455LysGlu: 5.455 ± 0.103	2.143LysPhe: 2.143 ± 0.055	4.242LysGly: 4.242 ± 0.075	0.828LysHis: 0.828 ± 0.032	5.665LysIle: 5.665 ± 0.097	5.562LysLys: 5.562 ± 0.106	4.36LysLeu: 4.36 ± 0.084	1.609LysMet: 1.609 ± 0.042	3.818LysAsn: 3.818 ± 0.081	2.251LysPro: 2.251 ± 0.052	1.248LysGln: 1.248 ± 0.044	3.124LysArg: 3.124 ± 0.071	4.405LysSer: 4.405 ± 0.082	3.582LysThr: 3.582 ± 0.07	3.346LysVal: 3.346 ± 0.062	0.589LysTrp: 0.589 ± 0.027	2.424LysTyr: 2.424 ± 0.059	0.0LysXaa: 0.0 ± 0.0
Leu	5.736LeuAla: 5.736 ± 0.094	1.136LeuCys: 1.136 ± 0.038	4.544LeuAsp: 4.544 ± 0.082	5.328LeuGlu: 5.328 ± 0.085	3.934LeuPhe: 3.934 ± 0.102	5.212LeuGly: 5.212 ± 0.087	1.456LeuHis: 1.456 ± 0.046	7.375LeuIle: 7.375 ± 0.117	6.775LeuLys: 6.775 ± 0.115	7.99LeuLeu: 7.99 ± 0.152	2.24LeuMet: 2.24 ± 0.053	4.488LeuAsn: 4.488 ± 0.081	3.491LeuPro: 3.491 ± 0.06	1.726LeuGln: 1.726 ± 0.052	4.08LeuArg: 4.08 ± 0.084	7.281LeuSer: 7.281 ± 0.089	4.631LeuThr: 4.631 ± 0.076	4.613LeuVal: 4.613 ± 0.084	0.775LeuTrp: 0.775 ± 0.034	2.81LeuTyr: 2.81 ± 0.063	0.0LeuXaa: 0.0 ± 0.0
Met	2.015MetAla: 2.015 ± 0.045	0.214MetCys: 0.214 ± 0.015	1.467MetAsp: 1.467 ± 0.037	1.607MetGlu: 1.607 ± 0.044	0.793MetPhe: 0.793 ± 0.032	1.663MetGly: 1.663 ± 0.046	0.46MetHis: 0.46 ± 0.02	2.066MetIle: 2.066 ± 0.052	2.012MetLys: 2.012 ± 0.054	2.212MetLeu: 2.212 ± 0.048	0.731MetMet: 0.731 ± 0.033	1.298MetAsn: 1.298 ± 0.034	1.221MetPro: 1.221 ± 0.039	0.707MetGln: 0.707 ± 0.03	1.155MetArg: 1.155 ± 0.04	1.628MetSer: 1.628 ± 0.047	1.58MetThr: 1.58 ± 0.039	1.537MetVal: 1.537 ± 0.048	0.182MetTrp: 0.182 ± 0.017	0.707MetTyr: 0.707 ± 0.028	0.0MetXaa: 0.0 ± 0.0
Asn	2.709AsnAla: 2.709 ± 0.059	0.623AsnCys: 0.623 ± 0.027	2.326AsnAsp: 2.326 ± 0.063	3.033AsnGlu: 3.033 ± 0.06	1.81AsnPhe: 1.81 ± 0.049	2.928AsnGly: 2.928 ± 0.078	0.686AsnHis: 0.686 ± 0.03	4.302AsnIle: 4.302 ± 0.08	2.528AsnLys: 2.528 ± 0.068	4.17AsnLeu: 4.17 ± 0.074	1.06AsnMet: 1.06 ± 0.034	2.472AsnAsn: 2.472 ± 0.065	2.784AsnPro: 2.784 ± 0.056	1.047AsnGln: 1.047 ± 0.036	2.24AsnArg: 2.24 ± 0.057	3.61AsnSer: 3.61 ± 0.075	2.326AsnThr: 2.326 ± 0.072	2.619AsnVal: 2.619 ± 0.059	0.43AsnTrp: 0.43 ± 0.024	1.917AsnTyr: 1.917 ± 0.054	0.0AsnXaa: 0.0 ± 0.0
Pro	3.166ProAla: 3.166 ± 0.065	0.452ProCys: 0.452 ± 0.022	3.706ProAsp: 3.706 ± 0.074	4.641ProGlu: 4.641 ± 0.076	1.658ProPhe: 1.658 ± 0.043	3.356ProGly: 3.356 ± 0.067	0.634ProHis: 0.634 ± 0.026	1.766ProIle: 1.766 ± 0.052	2.029ProLys: 2.029 ± 0.056	3.135ProLeu: 3.135 ± 0.062	0.766ProMet: 0.766 ± 0.025	1.06ProAsn: 1.06 ± 0.037	1.433ProPro: 1.433 ± 0.045	0.847ProGln: 0.847 ± 0.032	1.078ProArg: 1.078 ± 0.041	2.177ProSer: 2.177 ± 0.059	1.563ProThr: 1.563 ± 0.068	4.215ProVal: 4.215 ± 0.085	0.325ProTrp: 0.325 ± 0.023	1.477ProTyr: 1.477 ± 0.042	0.0ProXaa: 0.0 ± 0.0
Gln	1.406GlnAla: 1.406 ± 0.041	0.229GlnCys: 0.229 ± 0.019	1.139GlnAsp: 1.139 ± 0.035	1.404GlnGlu: 1.404 ± 0.047	0.811GlnPhe: 0.811 ± 0.033	1.207GlnGly: 1.207 ± 0.034	0.345GlnHis: 0.345 ± 0.021	1.884GlnIle: 1.884 ± 0.043	1.761GlnLys: 1.761 ± 0.049	1.587GlnLeu: 1.587 ± 0.044	0.661GlnMet: 0.661 ± 0.03	1.248GlnAsn: 1.248 ± 0.04	0.644GlnPro: 0.644 ± 0.028	0.547GlnGln: 0.547 ± 0.03	1.03GlnArg: 1.03 ± 0.033	1.382GlnSer: 1.382 ± 0.04	1.141GlnThr: 1.141 ± 0.035	1.33GlnVal: 1.33 ± 0.044	0.24GlnTrp: 0.24 ± 0.018	0.807GlnTyr: 0.807 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	2.888ArgAla: 2.888 ± 0.07	0.642ArgCys: 0.642 ± 0.025	3.069ArgAsp: 3.069 ± 0.065	4.129ArgGlu: 4.129 ± 0.083	1.954ArgPhe: 1.954 ± 0.054	2.962ArgGly: 2.962 ± 0.063	0.889ArgHis: 0.889 ± 0.037	4.205ArgIle: 4.205 ± 0.077	3.413ArgLys: 3.413 ± 0.068	3.783ArgLeu: 3.783 ± 0.069	1.427ArgMet: 1.427 ± 0.043	2.308ArgAsn: 2.308 ± 0.055	1.533ArgPro: 1.533 ± 0.043	1.243ArgGln: 1.243 ± 0.037	2.488ArgArg: 2.488 ± 0.065	2.631ArgSer: 2.631 ± 0.056	2.258ArgThr: 2.258 ± 0.052	2.811ArgVal: 2.811 ± 0.059	0.435ArgTrp: 0.435 ± 0.021	2.038ArgTyr: 2.038 ± 0.055	0.0ArgXaa: 0.0 ± 0.0
Ser	5.482SerAla: 5.482 ± 0.097	0.81SerCys: 0.81 ± 0.034	4.951SerAsp: 4.951 ± 0.081	6.131SerGlu: 6.131 ± 0.082	3.015SerPhe: 3.015 ± 0.064	8.44SerGly: 8.44 ± 0.138	1.116SerHis: 1.116 ± 0.034	4.3SerIle: 4.3 ± 0.072	3.384SerLys: 3.384 ± 0.063	6.012SerLeu: 6.012 ± 0.088	1.435SerMet: 1.435 ± 0.039	2.219SerAsn: 2.219 ± 0.058	2.97SerPro: 2.97 ± 0.07	1.53SerGln: 1.53 ± 0.035	3.121SerArg: 3.121 ± 0.053	5.059SerSer: 5.059 ± 0.1	2.924SerThr: 2.924 ± 0.07	5.534SerVal: 5.534 ± 0.093	0.608SerTrp: 0.608 ± 0.027	2.597SerTyr: 2.597 ± 0.059	0.0SerXaa: 0.0 ± 0.0
Thr	4.127ThrAla: 4.127 ± 0.093	0.518ThrCys: 0.518 ± 0.025	3.623ThrAsp: 3.623 ± 0.072	4.2ThrGlu: 4.2 ± 0.07	1.98ThrPhe: 1.98 ± 0.051	5.541ThrGly: 5.541 ± 0.103	0.793ThrHis: 0.793 ± 0.025	3.814ThrIle: 3.814 ± 0.078	2.511ThrLys: 2.511 ± 0.054	4.242ThrLeu: 4.242 ± 0.09	1.023ThrMet: 1.023 ± 0.036	1.644ThrAsn: 1.644 ± 0.052	2.608ThrPro: 2.608 ± 0.079	0.828ThrGln: 0.828 ± 0.032	1.814ThrArg: 1.814 ± 0.047	3.002ThrSer: 3.002 ± 0.079	2.457ThrThr: 2.457 ± 0.077	4.25ThrVal: 4.25 ± 0.113	0.409ThrTrp: 0.409 ± 0.024	1.674ThrTyr: 1.674 ± 0.057	0.0ThrXaa: 0.0 ± 0.0
Val	4.273ValAla: 4.273 ± 0.072	1.04ValCys: 1.04 ± 0.037	3.368ValAsp: 3.368 ± 0.076	3.763ValGlu: 3.763 ± 0.063	3.012ValPhe: 3.012 ± 0.07	3.642ValGly: 3.642 ± 0.085	1.156ValHis: 1.156 ± 0.039	6.036ValIle: 6.036 ± 0.098	3.985ValLys: 3.985 ± 0.076	5.807ValLeu: 5.807 ± 0.089	1.853ValMet: 1.853 ± 0.048	3.02ValAsn: 3.02 ± 0.07	2.898ValPro: 2.898 ± 0.061	1.398ValGln: 1.398 ± 0.046	3.406ValArg: 3.406 ± 0.085	5.322ValSer: 5.322 ± 0.11	3.905ValThr: 3.905 ± 0.099	4.301ValVal: 4.301 ± 0.085	0.568ValTrp: 0.568 ± 0.028	2.324ValTyr: 2.324 ± 0.056	0.0ValXaa: 0.0 ± 0.0
Trp	0.527TrpAla: 0.527 ± 0.027	0.11TrpCys: 0.11 ± 0.011	0.607TrpAsp: 0.607 ± 0.029	0.64TrpGlu: 0.64 ± 0.028	0.386TrpPhe: 0.386 ± 0.02	0.558TrpGly: 0.558 ± 0.028	0.178TrpHis: 0.178 ± 0.016	0.801TrpIle: 0.801 ± 0.029	0.6TrpLys: 0.6 ± 0.023	0.827TrpLeu: 0.827 ± 0.033	0.228TrpMet: 0.228 ± 0.017	0.571TrpAsn: 0.571 ± 0.029	0.219TrpPro: 0.219 ± 0.017	0.31TrpGln: 0.31 ± 0.025	0.453TrpArg: 0.453 ± 0.025	0.557TrpSer: 0.557 ± 0.028	0.457TrpThr: 0.457 ± 0.026	0.517TrpVal: 0.517 ± 0.029	0.153TrpTrp: 0.153 ± 0.015	0.365TrpTyr: 0.365 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.347TyrAla: 2.347 ± 0.057	0.517TyrCys: 0.517 ± 0.024	2.487TyrAsp: 2.487 ± 0.057	2.47TyrGlu: 2.47 ± 0.05	1.618TyrPhe: 1.618 ± 0.054	2.8TyrGly: 2.8 ± 0.066	0.686TyrHis: 0.686 ± 0.028	2.899TyrIle: 2.899 ± 0.057	1.601TyrLys: 1.601 ± 0.047	3.675TyrLeu: 3.675 ± 0.061	0.825TyrMet: 0.825 ± 0.029	1.926TyrAsn: 1.926 ± 0.054	1.666TyrPro: 1.666 ± 0.048	0.946TyrGln: 0.946 ± 0.036	1.934TyrArg: 1.934 ± 0.054	3.333TyrSer: 3.333 ± 0.07	1.9TyrThr: 1.9 ± 0.057	1.936TyrVal: 1.936 ± 0.054	0.333TyrTrp: 0.333 ± 0.018	1.77TyrTyr: 1.77 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2928 proteins (906721 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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