Pseudomonas phage JG004

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Pakpunavirus; Pseudomonas virus JG004

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 161 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|F4YDR6|F4YDR6_9CAUD Uncharacterized protein OS=Pseudomonas phage JG004 OX=757342 GN=PJG4_160 PE=4 SV=1
MM1 pKa = 7.62SILALTLTLCSAVQCDD17 pKa = 5.26DD18 pKa = 4.33YY19 pKa = 11.32IIDD22 pKa = 3.96HH23 pKa = 7.07ALPGQAAEE31 pKa = 4.32CNARR35 pKa = 11.84LVEE38 pKa = 4.15EE39 pKa = 4.31ADD41 pKa = 3.58EE42 pKa = 6.14FGDD45 pKa = 3.68AWVATNADD53 pKa = 3.58TRR55 pKa = 11.84LTRR58 pKa = 11.84YY59 pKa = 9.93LSRR62 pKa = 11.84FNIQVDD68 pKa = 3.36PRR70 pKa = 11.84FVFDD74 pKa = 5.54YY75 pKa = 11.21DD76 pKa = 3.86FTCQLIAEE84 pKa = 4.86DD85 pKa = 4.44EE86 pKa = 4.45LPP88 pKa = 4.02

Molecular weight: 9.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|F4YDT4|F4YDT4_9CAUD Uncharacterized protein OS=Pseudomonas phage JG004 OX=757342 GN=PJG4_170 PE=4 SV=1
MM1 pKa = 7.15NNKK4 pKa = 9.88YY5 pKa = 9.97KK6 pKa = 11.04DD7 pKa = 3.76NIIAEE12 pKa = 4.38YY13 pKa = 10.51CHH15 pKa = 6.71FDD17 pKa = 2.82GRR19 pKa = 11.84RR20 pKa = 11.84PVLSLAARR28 pKa = 11.84WVHH31 pKa = 5.92HH32 pKa = 6.72DD33 pKa = 3.29SRR35 pKa = 11.84VVKK38 pKa = 10.39LAALFACAGYY48 pKa = 10.0GRR50 pKa = 11.84PYY52 pKa = 10.01KK53 pKa = 10.68DD54 pKa = 4.4CIRR57 pKa = 11.84AYY59 pKa = 9.48KK60 pKa = 8.96AAKK63 pKa = 9.7RR64 pKa = 11.84RR65 pKa = 11.84EE66 pKa = 3.85KK67 pKa = 11.12AMRR70 pKa = 11.84NPATWKK76 pKa = 10.14LSRR79 pKa = 11.84EE80 pKa = 4.43AKK82 pKa = 10.27RR83 pKa = 11.84AVQWLQAAMNKK94 pKa = 9.67RR95 pKa = 11.84KK96 pKa = 9.94

Molecular weight: 11.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

161

0

161

27191

42

904

168.9

19.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.124 ± 0.235

1.405 ± 0.099

5.984 ± 0.157

7.602 ± 0.277

4.079 ± 0.112

7.087 ± 0.192

1.953 ± 0.13

5.447 ± 0.143

6.105 ± 0.224

7.925 ± 0.176

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.052 ± 0.125

4.134 ± 0.166

3.755 ± 0.161

3.556 ± 0.137

5.796 ± 0.168

5.881 ± 0.177

5.465 ± 0.196

7.171 ± 0.221

1.802 ± 0.103

3.678 ± 0.154
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski