Nakamurella panacisegetis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Nakamurellales; Nakamurellaceae; Nakamurella

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4387 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0MGR0|A0A1H0MGR0_9ACTN Uncharacterized protein OS=Nakamurella panacisegetis OX=1090615 GN=SAMN04515671_2009 PE=3 SV=1
MM1 pKa = 7.53EE2 pKa = 6.26FDD4 pKa = 3.99TGPDD8 pKa = 3.44VQPGHH13 pKa = 7.51DD14 pKa = 3.4ISISGSSGSTDD25 pKa = 3.35LGAATYY31 pKa = 10.75DD32 pKa = 3.57SDD34 pKa = 4.27HH35 pKa = 7.38DD36 pKa = 4.19GVADD40 pKa = 3.73SVIVIEE46 pKa = 5.26DD47 pKa = 3.2DD48 pKa = 3.35HH49 pKa = 8.37EE50 pKa = 4.36YY51 pKa = 11.12VITDD55 pKa = 3.56SDD57 pKa = 4.11HH58 pKa = 7.49DD59 pKa = 3.72GHH61 pKa = 7.38ADD63 pKa = 3.54SIHH66 pKa = 7.07AYY68 pKa = 10.17DD69 pKa = 3.84EE70 pKa = 4.6NGNQVDD76 pKa = 4.62PKK78 pKa = 9.96TGAPIDD84 pKa = 3.92GSGDD88 pKa = 3.27GTTGGSSDD96 pKa = 3.52GTTGTDD102 pKa = 3.06TGASTTDD109 pKa = 3.14HH110 pKa = 6.68TGSSTTGASTDD121 pKa = 3.46GTQTGSSSDD130 pKa = 3.59AGSDD134 pKa = 3.2ITVTGSDD141 pKa = 3.72GSAHH145 pKa = 6.9SIGTPTVDD153 pKa = 4.44LDD155 pKa = 4.03HH156 pKa = 7.59DD157 pKa = 4.92GSPDD161 pKa = 3.23TAVVHH166 pKa = 6.33NSDD169 pKa = 3.87GSVTGYY175 pKa = 8.75TDD177 pKa = 3.78RR178 pKa = 11.84DD179 pKa = 3.65GDD181 pKa = 3.81GHH183 pKa = 7.4ADD185 pKa = 3.55QITQIGADD193 pKa = 3.84GKK195 pKa = 10.59VVIAVADD202 pKa = 3.91NNGGWTITTTGHH214 pKa = 6.96LDD216 pKa = 3.05ASGNLVPDD224 pKa = 4.12GTPNPDD230 pKa = 3.4ASLDD234 pKa = 3.76PADD237 pKa = 3.95TAGVTSGHH245 pKa = 5.31TTTSGTATGASDD257 pKa = 3.59HH258 pKa = 5.79TTSGQTTSEE267 pKa = 3.97PTGDD271 pKa = 3.3GRR273 pKa = 11.84TVAEE277 pKa = 4.58PVPNSAPISGNEE289 pKa = 3.74PDD291 pKa = 3.84GDD293 pKa = 3.7ITFSEE298 pKa = 4.5DD299 pKa = 3.42GKK301 pKa = 10.72DD302 pKa = 3.44YY303 pKa = 11.43DD304 pKa = 4.79LGHH307 pKa = 7.23PTTDD311 pKa = 3.64LDD313 pKa = 4.86GDD315 pKa = 4.1GTPDD319 pKa = 3.19TVVTHH324 pKa = 6.83LADD327 pKa = 3.77GTVVGYY333 pKa = 9.39TDD335 pKa = 3.46TDD337 pKa = 3.71GDD339 pKa = 3.92GTADD343 pKa = 3.9QITQISAGGQVVIGVSDD360 pKa = 3.5GHH362 pKa = 6.68GGWTQAATGHH372 pKa = 6.09MGADD376 pKa = 3.6GKK378 pKa = 10.39FVPDD382 pKa = 3.77SSAAATTGG390 pKa = 3.1

Molecular weight: 38.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0NTZ7|A0A1H0NTZ7_9ACTN Isochorismate synthase OS=Nakamurella panacisegetis OX=1090615 GN=SAMN04515671_2480 PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.7LLRR22 pKa = 11.84KK23 pKa = 7.81TRR25 pKa = 11.84VARR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.68AGKK33 pKa = 9.73

Molecular weight: 4.0 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4387

0

4387

1470634

29

3217

335.2

35.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.449 ± 0.049

0.693 ± 0.01

5.99 ± 0.032

4.527 ± 0.044

2.851 ± 0.024

9.317 ± 0.036

2.06 ± 0.019

4.174 ± 0.022

2.02 ± 0.028

9.927 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.827 ± 0.015

2.082 ± 0.021

5.937 ± 0.033

2.909 ± 0.018

7.172 ± 0.05

6.058 ± 0.035

6.654 ± 0.052

8.971 ± 0.035

1.426 ± 0.017

1.956 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski