bacterium D16-54

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5047 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9BQI8|A0A3A9BQI8_9BACT Uncharacterized protein OS=bacterium D16-54 OX=2320104 GN=D7X87_08745 PE=4 SV=1
MM1 pKa = 7.98VIDD4 pKa = 3.34SWEE7 pKa = 3.88NGQYY11 pKa = 10.41KK12 pKa = 9.43EE13 pKa = 3.33IWVYY17 pKa = 11.08SKK19 pKa = 10.63EE20 pKa = 4.25AEE22 pKa = 4.05DD23 pKa = 5.75DD24 pKa = 3.6EE25 pKa = 5.74RR26 pKa = 11.84AMCGLINGDD35 pKa = 3.92WGWLNYY41 pKa = 10.16YY42 pKa = 10.3NEE44 pKa = 4.63EE45 pKa = 3.78GDD47 pKa = 3.88AGLSSRR53 pKa = 11.84NPNYY57 pKa = 10.27TDD59 pKa = 3.29TDD61 pKa = 3.69DD62 pKa = 5.19DD63 pKa = 3.71EE64 pKa = 4.74TMNFIINGEE73 pKa = 4.18LDD75 pKa = 4.38PFPLSYY81 pKa = 10.81VLPAEE86 pKa = 4.21QVMEE90 pKa = 4.09ALEE93 pKa = 4.1YY94 pKa = 10.71FEE96 pKa = 5.84KK97 pKa = 10.55FHH99 pKa = 6.94KK100 pKa = 10.69LPTFITWHH108 pKa = 7.37DD109 pKa = 3.88DD110 pKa = 3.16NFAA113 pKa = 4.8

Molecular weight: 13.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9BJH3|A0A3A9BJH3_9BACT Peptidase_M56 domain-containing protein OS=bacterium D16-54 OX=2320104 GN=D7X87_14745 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.73MTFQPKK8 pKa = 8.63NRR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 8.89VHH16 pKa = 5.88GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTPGGRR28 pKa = 11.84KK29 pKa = 8.77VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.43GRR39 pKa = 11.84AKK41 pKa = 10.69LSAA44 pKa = 3.92

Molecular weight: 4.98 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5047

0

5047

1530207

26

4532

303.2

34.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.426 ± 0.043

1.559 ± 0.016

5.495 ± 0.03

7.763 ± 0.047

4.114 ± 0.027

7.496 ± 0.043

1.772 ± 0.016

6.811 ± 0.034

6.301 ± 0.035

9.041 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.165 ± 0.023

4.032 ± 0.025

3.4 ± 0.019

3.652 ± 0.022

5.296 ± 0.035

5.827 ± 0.028

4.93 ± 0.028

6.625 ± 0.033

1.101 ± 0.013

4.194 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski