Anoxybacillus flavithermus (strain DSM 21510 / WK1)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Anoxybacillus; Anoxybacillus flavithermus

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2819 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7GIX4|B7GIX4_ANOFW Uncharacterized protein OS=Anoxybacillus flavithermus (strain DSM 21510 / WK1) OX=491915 GN=Aflv_0787 PE=4 SV=1
MM1 pKa = 8.11NMMEE5 pKa = 4.91HH6 pKa = 6.51GEE8 pKa = 3.97KK9 pKa = 10.35HH10 pKa = 4.59ITVVDD15 pKa = 3.6EE16 pKa = 5.22DD17 pKa = 4.57GNEE20 pKa = 3.96QLCEE24 pKa = 3.79VLFTFEE30 pKa = 4.39SEE32 pKa = 4.46EE33 pKa = 3.99FGKK36 pKa = 10.71SYY38 pKa = 11.04VLYY41 pKa = 9.7YY42 pKa = 10.09PIGAEE47 pKa = 3.67EE48 pKa = 4.98DD49 pKa = 3.73DD50 pKa = 3.95NEE52 pKa = 4.5EE53 pKa = 4.14IEE55 pKa = 4.08IHH57 pKa = 6.26ASSFIPGEE65 pKa = 4.3DD66 pKa = 3.41GQAGEE71 pKa = 4.39LQPIEE76 pKa = 4.47TEE78 pKa = 4.44EE79 pKa = 4.01EE80 pKa = 3.37WDD82 pKa = 3.83MIEE85 pKa = 4.74EE86 pKa = 4.03MLNTFLGEE94 pKa = 4.15EE95 pKa = 4.21EE96 pKa = 4.46EE97 pKa = 4.48EE98 pKa = 4.26

Molecular weight: 11.33 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7GEW9|B7GEW9_ANOFW Permease of the major facilitator superfamily OS=Anoxybacillus flavithermus (strain DSM 21510 / WK1) OX=491915 GN=Aflv_2325 PE=3 SV=1
MM1 pKa = 8.23SYY3 pKa = 9.75MKK5 pKa = 9.74RR6 pKa = 11.84TYY8 pKa = 10.27QPNKK12 pKa = 8.21RR13 pKa = 11.84KK14 pKa = 9.62RR15 pKa = 11.84SKK17 pKa = 9.83VHH19 pKa = 5.77GFRR22 pKa = 11.84ARR24 pKa = 11.84MSTKK28 pKa = 10.19NGRR31 pKa = 11.84KK32 pKa = 8.49VLARR36 pKa = 11.84RR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84KK40 pKa = 9.05GRR42 pKa = 11.84KK43 pKa = 8.7VLSAA47 pKa = 4.05

Molecular weight: 5.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2819

0

2819

830520

23

1990

294.6

33.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.456 ± 0.051

0.875 ± 0.015

4.775 ± 0.038

7.421 ± 0.052

4.518 ± 0.041

6.483 ± 0.05

2.595 ± 0.028

7.37 ± 0.042

6.594 ± 0.043

9.426 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.0 ± 0.023

3.604 ± 0.036

3.641 ± 0.028

4.342 ± 0.038

5.02 ± 0.036

4.979 ± 0.031

5.494 ± 0.035

7.67 ± 0.041

1.089 ± 0.019

3.648 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski