Enterocytozoon bieneusi (strain H348) (Microsporidian parasite)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Microsporidia; Apansporoblastina; Enterocytozoonidae; Enterocytozoon; Enterocytozoon bieneusi

Average proteome isoelectric point is 7.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3311 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B7XMF2|B7XMF2_ENTBH Uncharacterized protein (Fragment) OS=Enterocytozoon bieneusi (strain H348) OX=481877 GN=EBI_25618 PE=4 SV=1
MM1 pKa = 7.43TSSDD5 pKa = 3.99CDD7 pKa = 3.17NGEE10 pKa = 3.91IMKK13 pKa = 7.87TTTPIYY19 pKa = 10.73AVDD22 pKa = 3.54VKK24 pKa = 11.24GNYY27 pKa = 9.42ILVGGKK33 pKa = 9.67DD34 pKa = 3.53EE35 pKa = 4.23NAAVYY40 pKa = 9.05TVDD43 pKa = 3.83NGDD46 pKa = 5.06LITIVADD53 pKa = 3.71LEE55 pKa = 4.26EE56 pKa = 4.94SVMFCKK62 pKa = 10.2MLDD65 pKa = 3.7DD66 pKa = 5.02HH67 pKa = 7.4DD68 pKa = 4.2VLYY71 pKa = 11.07LVVTNDD77 pKa = 4.49GLICPMKK84 pKa = 10.41LDD86 pKa = 3.53QSVGNTT92 pKa = 3.26

Molecular weight: 9.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B7XP35|B7XP35_ENTBH Uncharacterized protein OS=Enterocytozoon bieneusi (strain H348) OX=481877 GN=EBI_26537 PE=4 SV=1
MM1 pKa = 7.04QGKK4 pKa = 9.49KK5 pKa = 9.87KK6 pKa = 10.36FQIPPPKK13 pKa = 9.99IPGGRR18 pKa = 11.84LRR20 pKa = 11.84NNFLPKK26 pKa = 10.25KK27 pKa = 9.95KK28 pKa = 10.41FGGRR32 pKa = 11.84KK33 pKa = 9.06RR34 pKa = 11.84GLGVFF39 pKa = 4.15

Molecular weight: 4.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3311

0

3311

826748

29

2166

249.7

28.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.837 ± 0.051

1.94 ± 0.024

5.182 ± 0.03

6.6 ± 0.05

5.427 ± 0.037

4.249 ± 0.05

2.026 ± 0.018

10.329 ± 0.068

9.191 ± 0.057

9.274 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.347 ± 0.016

7.629 ± 0.055

2.946 ± 0.033

3.331 ± 0.023

3.489 ± 0.04

6.334 ± 0.039

5.402 ± 0.033

5.118 ± 0.036

0.73 ± 0.013

4.605 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski