Dendroctonus ponderosae (Mountain pine beetle)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Coleoptera; Polyphaga; Cucujiformia; Curculionoidea; Curculionidae; Scolytinae;

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12251 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U4UE00|U4UE00_DENPD Myb-like SWIRM and MPN domain-containing protein 1 OS=Dendroctonus ponderosae OX=77166 GN=D910_06198 PE=3 SV=1
MM1 pKa = 7.48FYY3 pKa = 10.33WILILLFAPSLVNTLTCHH21 pKa = 6.21RR22 pKa = 11.84CFGYY26 pKa = 10.34GFCHH30 pKa = 7.5DD31 pKa = 4.92PDD33 pKa = 6.15DD34 pKa = 5.25PDD36 pKa = 4.11FSDD39 pKa = 5.29GIRR42 pKa = 11.84VAACSSWKK50 pKa = 10.52YY51 pKa = 9.05WDD53 pKa = 4.3EE54 pKa = 4.28PLLSDD59 pKa = 4.63DD60 pKa = 3.52PFGRR64 pKa = 11.84RR65 pKa = 11.84VEE67 pKa = 4.0EE68 pKa = 4.12ALNEE72 pKa = 4.11NYY74 pKa = 10.09RR75 pKa = 11.84HH76 pKa = 6.03NFLNSSTQNTQLEE89 pKa = 4.65HH90 pKa = 6.4HH91 pKa = 6.85CVTARR96 pKa = 11.84VHH98 pKa = 4.92EE99 pKa = 4.49TKK101 pKa = 10.69NSYY104 pKa = 7.29TLRR107 pKa = 11.84TCISRR112 pKa = 11.84VVPTDD117 pKa = 2.93QSYY120 pKa = 7.64PTLCEE125 pKa = 4.95LIDD128 pKa = 6.39DD129 pKa = 4.9LTQSQNRR136 pKa = 11.84HH137 pKa = 5.62NGSFSCRR144 pKa = 11.84QCTGDD149 pKa = 3.33FCNTHH154 pKa = 6.91ALQEE158 pKa = 4.4GDD160 pKa = 4.0GVAIDD165 pKa = 5.92DD166 pKa = 5.79DD167 pKa = 6.69DD168 pKa = 7.58DD169 pKa = 7.56DD170 pKa = 7.6DD171 pKa = 7.67DD172 pKa = 7.44DD173 pKa = 5.66DD174 pKa = 5.47TDD176 pKa = 6.4DD177 pKa = 6.64DD178 pKa = 6.57DD179 pKa = 7.84DD180 pKa = 7.67DD181 pKa = 7.56DD182 pKa = 7.61DD183 pKa = 7.73DD184 pKa = 7.59DD185 pKa = 6.71DD186 pKa = 7.23DD187 pKa = 6.46SDD189 pKa = 5.76NEE191 pKa = 4.16TDD193 pKa = 4.27DD194 pKa = 5.45LKK196 pKa = 11.47DD197 pKa = 3.32SASTVTRR204 pKa = 11.84VAFTVLAFCSVSIGLSLFSDD224 pKa = 4.19

Molecular weight: 25.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U4UHD8|U4UHD8_DENPD Uncharacterized protein OS=Dendroctonus ponderosae OX=77166 GN=D910_09741 PE=4 SV=1
MM1 pKa = 6.95WTYY4 pKa = 9.21QACICPKK11 pKa = 9.89IRR13 pKa = 11.84SCVGKK18 pKa = 9.52WPWASIRR25 pKa = 11.84RR26 pKa = 11.84YY27 pKa = 10.07HH28 pKa = 7.66DD29 pKa = 3.45GGDD32 pKa = 3.76RR33 pKa = 11.84NDD35 pKa = 3.17SRR37 pKa = 11.84ARR39 pKa = 11.84GCAQQPHH46 pKa = 6.93LDD48 pKa = 3.4HH49 pKa = 7.02RR50 pKa = 11.84RR51 pKa = 11.84STFHH55 pKa = 6.86RR56 pKa = 11.84ANRR59 pKa = 11.84PKK61 pKa = 10.69ALEE64 pKa = 4.34DD65 pKa = 3.27RR66 pKa = 11.84SEE68 pKa = 4.07AGKK71 pKa = 10.46CLSVWLSMSRR81 pKa = 11.84LPSNRR86 pKa = 11.84RR87 pKa = 11.84ILQSKK92 pKa = 9.1

Molecular weight: 10.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12069

182

12251

5412742

10

13998

441.8

49.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.448 ± 0.021

2.004 ± 0.042

5.436 ± 0.018

6.706 ± 0.033

4.048 ± 0.017

5.609 ± 0.029

2.441 ± 0.012

5.736 ± 0.017

6.642 ± 0.031

9.44 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.011

4.946 ± 0.019

5.065 ± 0.033

4.376 ± 0.019

5.019 ± 0.02

7.807 ± 0.024

5.478 ± 0.02

6.233 ± 0.018

1.114 ± 0.007

3.203 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski