Hubei dimarhabdovirus virus 3

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KMV3|A0A1L3KMV3_9VIRU Uncharacterized protein OS=Hubei dimarhabdovirus virus 3 OX=1922868 PE=4 SV=1
MM1 pKa = 7.7ALNFSDD7 pKa = 4.08EE8 pKa = 4.42RR9 pKa = 11.84FTATSQSMISLYY21 pKa = 10.77EE22 pKa = 3.95SLTNRR27 pKa = 11.84QRR29 pKa = 11.84EE30 pKa = 3.88ILEE33 pKa = 4.74RR34 pKa = 11.84IDD36 pKa = 5.53ANTLSNEE43 pKa = 3.94SLYY46 pKa = 11.34NLDD49 pKa = 3.26QEE51 pKa = 4.69LAAFVPEE58 pKa = 4.15SLPRR62 pKa = 11.84EE63 pKa = 4.22ASPQFPADD71 pKa = 3.35AFRR74 pKa = 11.84AKK76 pKa = 9.06PTVVLYY82 pKa = 10.66SVEE85 pKa = 3.9KK86 pKa = 10.76ADD88 pKa = 3.32VHH90 pKa = 6.61KK91 pKa = 10.91YY92 pKa = 7.66YY93 pKa = 9.17HH94 pKa = 5.88TVMASFRR101 pKa = 11.84EE102 pKa = 4.3KK103 pKa = 10.48LVHH106 pKa = 6.55PKK108 pKa = 9.35MLATFLHH115 pKa = 6.94LSMMTYY121 pKa = 9.69QVEE124 pKa = 4.37LTATLPTAWTSFSIPLTDD142 pKa = 3.87GSLNTSPLHH151 pKa = 6.76LYY153 pKa = 7.82TATAVGTPTLVEE165 pKa = 4.19STVPATEE172 pKa = 3.95KK173 pKa = 10.63AIKK176 pKa = 9.7ALVMVVVGFIRR187 pKa = 11.84YY188 pKa = 9.67SLMIEE193 pKa = 3.84NLGDD197 pKa = 3.29YY198 pKa = 10.21RR199 pKa = 11.84RR200 pKa = 11.84NFNTRR205 pKa = 11.84LTEE208 pKa = 3.99MVKK211 pKa = 10.67SIDD214 pKa = 3.49PDD216 pKa = 4.0FPADD220 pKa = 3.33VSSYY224 pKa = 10.89SGIRR228 pKa = 11.84GMNNFFPDD236 pKa = 3.8LGLVAAALDD245 pKa = 4.29MYY247 pKa = 8.5TVRR250 pKa = 11.84VPQSEE255 pKa = 3.7WRR257 pKa = 11.84IFNMGTLVLRR267 pKa = 11.84NMSAAALVAVTYY279 pKa = 9.59MRR281 pKa = 11.84EE282 pKa = 3.92IMNSTEE288 pKa = 4.02SNHH291 pKa = 6.21YY292 pKa = 7.18YY293 pKa = 10.52HH294 pKa = 6.68SWIWVQSAADD304 pKa = 4.18EE305 pKa = 4.28YY306 pKa = 11.08AKK308 pKa = 10.19MMRR311 pKa = 11.84SGQEE315 pKa = 3.38INNLYY320 pKa = 10.65SYY322 pKa = 11.29APYY325 pKa = 11.36LMTLGKK331 pKa = 10.01AVKK334 pKa = 9.57SPYY337 pKa = 9.98SATVNSNLFTFIHH350 pKa = 6.38VIGASLGLPRR360 pKa = 11.84SINSRR365 pKa = 11.84QLDD368 pKa = 3.97HH369 pKa = 7.26INLNEE374 pKa = 4.53LIINAQFYY382 pKa = 10.66VFAAQRR388 pKa = 11.84APRR391 pKa = 11.84FNMHH395 pKa = 5.47VVNDD399 pKa = 4.05EE400 pKa = 4.13IIRR403 pKa = 11.84ALKK406 pKa = 9.67EE407 pKa = 3.3IHH409 pKa = 6.53AGNRR413 pKa = 11.84AAVGQLAEE421 pKa = 3.77IRR423 pKa = 11.84GRR425 pKa = 11.84LVEE428 pKa = 4.51NDD430 pKa = 3.72DD431 pKa = 3.99EE432 pKa = 4.45EE433 pKa = 4.74MEE435 pKa = 4.85IEE437 pKa = 4.74DD438 pKa = 4.04SASVAGTNDD447 pKa = 2.87LTQFQTAVDD456 pKa = 3.71HH457 pKa = 5.88EE458 pKa = 4.69AYY460 pKa = 9.21PRR462 pKa = 11.84SSSGVEE468 pKa = 3.36WSSWYY473 pKa = 10.0FRR475 pKa = 11.84NKK477 pKa = 9.27VVPEE481 pKa = 3.93EE482 pKa = 4.08VKK484 pKa = 10.83EE485 pKa = 4.01NCQKK489 pKa = 10.0VWRR492 pKa = 11.84QFVTPRR498 pKa = 11.84NNSLGSVLKK507 pKa = 10.76NLSS510 pKa = 3.21

Molecular weight: 57.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KMX0|A0A1L3KMX0_9VIRU Uncharacterized protein OS=Hubei dimarhabdovirus virus 3 OX=1922868 PE=4 SV=1
MM1 pKa = 7.68KK2 pKa = 9.15KK3 pKa = 8.68TNKK6 pKa = 6.8NHH8 pKa = 5.68KK9 pKa = 8.56MKK11 pKa = 10.33RR12 pKa = 11.84LNRR15 pKa = 11.84IRR17 pKa = 11.84SLLILLIIGEE27 pKa = 4.4RR28 pKa = 11.84TSSILLPSYY37 pKa = 10.09EE38 pKa = 4.0PKK40 pKa = 9.5QWEE43 pKa = 4.26RR44 pKa = 11.84VNPIDD49 pKa = 4.67LSCPRR54 pKa = 11.84IEE56 pKa = 4.31SVKK59 pKa = 10.27TEE61 pKa = 4.08VVDD64 pKa = 4.78ISPSQCSRR72 pKa = 11.84VSRR75 pKa = 11.84ALTRR79 pKa = 11.84QEE81 pKa = 3.85SGFWCIGVVMITKK94 pKa = 8.85CTEE97 pKa = 3.7SCFGGTDD104 pKa = 3.37VVRR107 pKa = 11.84VSRR110 pKa = 11.84DD111 pKa = 3.15NPVSYY116 pKa = 9.67TEE118 pKa = 5.13CSDD121 pKa = 3.88ALQDD125 pKa = 3.82FLKK128 pKa = 10.88GQLSFPAFPPAQCSWCSSNSEE149 pKa = 3.98EE150 pKa = 4.54AKK152 pKa = 10.67SILIVKK158 pKa = 9.81EE159 pKa = 4.23DD160 pKa = 3.46IPYY163 pKa = 10.6EE164 pKa = 4.15PLTNLYY170 pKa = 9.19PLGGIDD176 pKa = 3.98PTSCDD181 pKa = 3.04NHH183 pKa = 6.55HH184 pKa = 7.06CLTNSRR190 pKa = 11.84SRR192 pKa = 11.84MWMRR196 pKa = 11.84WTTNSEE202 pKa = 4.23SCFLQPEE209 pKa = 4.3NFLLLRR215 pKa = 11.84NRR217 pKa = 11.84TGSYY221 pKa = 10.51LRR223 pKa = 11.84DD224 pKa = 3.77SVHH227 pKa = 5.69RR228 pKa = 11.84TYY230 pKa = 11.55SLLSACLTDD239 pKa = 3.68YY240 pKa = 10.85CHH242 pKa = 7.39RR243 pKa = 11.84PTLYY247 pKa = 10.77LSGRR251 pKa = 11.84EE252 pKa = 3.96LLSCEE257 pKa = 4.74SVLLDD262 pKa = 3.32QLKK265 pKa = 10.63LKK267 pKa = 10.28KK268 pKa = 10.04CPKK271 pKa = 8.48QIRR274 pKa = 11.84YY275 pKa = 9.45EE276 pKa = 3.96IDD278 pKa = 3.24PANQDD283 pKa = 3.41VLAEE287 pKa = 4.02DD288 pKa = 3.68QFIQRR293 pKa = 11.84EE294 pKa = 4.21LVQVKK299 pKa = 10.45CEE301 pKa = 3.98DD302 pKa = 3.17SRR304 pKa = 11.84EE305 pKa = 3.88RR306 pKa = 11.84VIKK309 pKa = 10.31GGSIAPSDD317 pKa = 4.25LYY319 pKa = 11.69LMSKK323 pKa = 9.91KK324 pKa = 10.69GPGEE328 pKa = 4.11GYY330 pKa = 10.91VYY332 pKa = 10.53FVDD335 pKa = 3.41NAGVLKK341 pKa = 10.73RR342 pKa = 11.84GLTNYY347 pKa = 10.1RR348 pKa = 11.84EE349 pKa = 4.14VVAAEE354 pKa = 4.09SMVNLTSSLSSSEE367 pKa = 4.21TIKK370 pKa = 10.39WSLWTKK376 pKa = 7.86LTPPGLFMGPNGIVWDD392 pKa = 4.08EE393 pKa = 3.93TSEE396 pKa = 4.12RR397 pKa = 11.84LSFPSLFYY405 pKa = 11.22DD406 pKa = 3.68RR407 pKa = 11.84VLNLDD412 pKa = 3.48VKK414 pKa = 10.05LANMTQTLSDD424 pKa = 3.8GADD427 pKa = 3.47HH428 pKa = 5.79QTHH431 pKa = 7.15PGLGSLLKK439 pKa = 11.07NMILQHH445 pKa = 6.07DD446 pKa = 4.07HH447 pKa = 6.67SKK449 pKa = 11.34NILDD453 pKa = 3.92VVATAGGDD461 pKa = 3.74LVTSTILTVTKK472 pKa = 10.24CVLYY476 pKa = 10.4ILGLIIFIQVTACVLRR492 pKa = 11.84KK493 pKa = 9.57VLCSPKK499 pKa = 10.04KK500 pKa = 9.91GKK502 pKa = 8.06TPQGYY507 pKa = 9.95DD508 pKa = 2.93LVRR511 pKa = 11.84YY512 pKa = 9.99VNGPQAAVSISKK524 pKa = 10.38ASKK527 pKa = 9.91

Molecular weight: 59.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3740

224

2134

748.0

85.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.856 ± 0.985

1.845 ± 0.459

5.241 ± 0.289

6.337 ± 0.329

4.358 ± 0.445

4.947 ± 0.405

2.888 ± 0.417

6.123 ± 0.685

5.134 ± 0.563

9.893 ± 0.651

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.807 ± 0.294

4.786 ± 0.366

5.08 ± 0.667

3.583 ± 0.295

5.963 ± 0.208

8.85 ± 0.539

5.668 ± 0.222

5.668 ± 0.643

1.364 ± 0.16

3.61 ± 0.302
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski