Pseudomonas phage GP100

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Zobellviridae; unclassified Zobellviridae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P9FI82|A0A2P9FI82_9CAUD Uncharacterized protein OS=Pseudomonas phage GP100 OX=2055238 PE=4 SV=1
MM1 pKa = 6.86YY2 pKa = 10.38VKK4 pKa = 10.47EE5 pKa = 4.2ADD7 pKa = 4.17RR8 pKa = 11.84EE9 pKa = 4.36VGTCTEE15 pKa = 4.11CMQVGEE21 pKa = 4.14LNEE24 pKa = 4.57RR25 pKa = 11.84GVCDD29 pKa = 4.31FCEE32 pKa = 4.24DD33 pKa = 3.83PYY35 pKa = 11.64GRR37 pKa = 11.84SALAMLLDD45 pKa = 3.96LEE47 pKa = 4.34EE48 pKa = 6.11AILHH52 pKa = 5.2GQEE55 pKa = 3.85

Molecular weight: 6.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P9FID1|A0A2P9FID1_9CAUD Metallophosphatase domain protein OS=Pseudomonas phage GP100 OX=2055238 PE=4 SV=1
MM1 pKa = 7.43AAVSTIVAVAAVAVAAGSMYY21 pKa = 10.16MSSQEE26 pKa = 3.84AKK28 pKa = 10.48AAAKK32 pKa = 9.93DD33 pKa = 3.68RR34 pKa = 11.84KK35 pKa = 9.54QASQVSQAEE44 pKa = 4.2SAAQRR49 pKa = 11.84NQNRR53 pKa = 11.84RR54 pKa = 11.84QQVRR58 pKa = 11.84EE59 pKa = 3.72EE60 pKa = 4.05RR61 pKa = 11.84VRR63 pKa = 11.84RR64 pKa = 11.84AQIMQGSQNTGVSQSSGEE82 pKa = 4.07LGATSALGTLISSNVATQSRR102 pKa = 11.84QQNSSDD108 pKa = 5.62AIASWNQRR116 pKa = 11.84AADD119 pKa = 3.97SDD121 pKa = 4.17LASQQWSGIGSIAGSVFGVAANMSMASKK149 pKa = 10.83APTSVKK155 pKa = 8.98PTTGGSSFGTAAGNSLFNN173 pKa = 4.36

Molecular weight: 17.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15418

34

1595

202.9

22.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.977 ± 0.46

1.187 ± 0.183

6.59 ± 0.248

6.376 ± 0.316

3.82 ± 0.139

7.537 ± 0.25

2.017 ± 0.187

5.189 ± 0.19

6.382 ± 0.342

8.257 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.374 ± 0.137

4.462 ± 0.178

3.937 ± 0.244

4.119 ± 0.291

4.929 ± 0.24

5.558 ± 0.312

5.591 ± 0.362

7.478 ± 0.25

1.615 ± 0.139

3.606 ± 0.148
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski