Haemophilus parasuis serovar 5 (strain SH0165) (Glaesserella parasuis)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Glaesserella; Glaesserella parasuis

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2002 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|B8F710|B8F710_HAEPS Putative phage associated protein OS=Haemophilus parasuis serovar 5 (strain SH0165) OX=557723 GN=HAPS_1558 PE=4 SV=1
MM1 pKa = 7.53SKK3 pKa = 10.22SICIITGSTLGGAEE17 pKa = 3.95YY18 pKa = 10.87VADD21 pKa = 4.16HH22 pKa = 6.75LNDD25 pKa = 3.13VLTEE29 pKa = 3.62QGYY32 pKa = 8.38QVGLFNQAEE41 pKa = 4.19LADD44 pKa = 4.13IQHH47 pKa = 5.07QTALLVVTSTHH58 pKa = 6.42GAGEE62 pKa = 4.35LPEE65 pKa = 5.07NIQPLFQQLAEE76 pKa = 4.49SNLDD80 pKa = 3.43LSQMKK85 pKa = 10.16FGVVGLGSSDD95 pKa = 3.48YY96 pKa = 10.8DD97 pKa = 3.85TFCNAVNLVEE107 pKa = 4.51QGLTAKK113 pKa = 10.54GATQVCEE120 pKa = 4.0SLRR123 pKa = 11.84IDD125 pKa = 3.44VVNNFDD131 pKa = 3.83HH132 pKa = 7.56DD133 pKa = 4.21GSAEE137 pKa = 3.8EE138 pKa = 4.28WLPAFTSTLL147 pKa = 3.51

Molecular weight:
15.75 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|B8F7V6|B8F7V6_HAEPS Transcriptional regulator OS=Haemophilus parasuis serovar 5 (strain SH0165) OX=557723 GN=HAPS_1920 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.49RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.91GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight:
5.08 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2002

0

2002

604561

35

2159

302.0

33.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.415 ± 0.062

0.991 ± 0.02

5.011 ± 0.039

6.565 ± 0.062

4.315 ± 0.051

6.829 ± 0.102

2.069 ± 0.026

6.886 ± 0.045

6.286 ± 0.054

10.317 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.372 ± 0.023

4.648 ± 0.038

3.821 ± 0.039

4.918 ± 0.048

4.51 ± 0.045

5.737 ± 0.043

5.248 ± 0.042

6.748 ± 0.05

1.133 ± 0.022

3.182 ± 0.03

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski