Salmonella phage vB_SenS-Ent3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; unclassified Jerseyvirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 60 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|X5JM42|X5JM42_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS-Ent3 OX=1465613 GN=SEP3_gp59 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.39VYY4 pKa = 10.11IVSGWYY10 pKa = 9.7HH11 pKa = 6.7YY12 pKa = 11.14EE13 pKa = 3.64DD14 pKa = 4.16TYY16 pKa = 11.88VLGVYY21 pKa = 10.59LSEE24 pKa = 4.44EE25 pKa = 4.09KK26 pKa = 11.09AEE28 pKa = 4.11AAAEE32 pKa = 4.14LEE34 pKa = 4.23RR35 pKa = 11.84SKK37 pKa = 10.95QYY39 pKa = 11.06YY40 pKa = 10.49DD41 pKa = 3.62GVDD44 pKa = 3.25VCGWEE49 pKa = 4.11VQEE52 pKa = 4.48

Molecular weight: 6.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|X5JRT9|X5JRT9_9CAUD Uncharacterized protein OS=Salmonella phage vB_SenS-Ent3 OX=1465613 GN=SEP3_gp53 PE=4 SV=1
MM1 pKa = 7.52SLATDD6 pKa = 3.7ILKK9 pKa = 10.24HH10 pKa = 6.28AGINLAPPSSAVTKK24 pKa = 10.47KK25 pKa = 7.25VTRR28 pKa = 11.84PKK30 pKa = 10.94EE31 pKa = 3.46EE32 pKa = 4.49DD33 pKa = 2.93KK34 pKa = 11.01RR35 pKa = 11.84KK36 pKa = 10.0RR37 pKa = 11.84KK38 pKa = 9.09PKK40 pKa = 10.04PRR42 pKa = 11.84VKK44 pKa = 10.24LVNEE48 pKa = 4.24MPDD51 pKa = 3.38VYY53 pKa = 10.73PRR55 pKa = 11.84IPGVHH60 pKa = 4.6QPKK63 pKa = 9.16YY64 pKa = 10.53CVGKK68 pKa = 10.16GLWRR72 pKa = 11.84AHH74 pKa = 6.43SYY76 pKa = 10.54DD77 pKa = 3.01GKK79 pKa = 10.98KK80 pKa = 10.05VVNLGEE86 pKa = 4.35FSSQARR92 pKa = 11.84AHH94 pKa = 6.01MAVKK98 pKa = 10.46LYY100 pKa = 10.67KK101 pKa = 9.81LWRR104 pKa = 11.84KK105 pKa = 9.46RR106 pKa = 11.84GYY108 pKa = 10.35SEE110 pKa = 5.16IPHH113 pKa = 6.33KK114 pKa = 10.54PSIRR118 pKa = 11.84LYY120 pKa = 8.83TFRR123 pKa = 6.0

Molecular weight: 14.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

60

0

60

13129

47

1032

218.8

24.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.521 ± 0.531

1.059 ± 0.137

6.078 ± 0.204

6.703 ± 0.374

3.755 ± 0.175

7.716 ± 0.305

1.607 ± 0.154

4.768 ± 0.189

6.025 ± 0.343

7.822 ± 0.296

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.689 ± 0.173

4.364 ± 0.224

3.793 ± 0.201

3.717 ± 0.23

5.659 ± 0.237

5.956 ± 0.281

6.429 ± 0.328

7.426 ± 0.299

1.5 ± 0.142

3.412 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski