Paracoccus sp. 228

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Paracoccus; unclassified Paracoccus

Average proteome isoelectric point is 6.38

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3463 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0D6TAR2|A0A0D6TAR2_9RHOB Protein-methionine-sulfoxide reductase catalytic subunit MsrP OS=Paracoccus sp. 228 OX=1192054 GN=msrP PE=3 SV=1
MM1 pKa = 7.48KK2 pKa = 10.09KK3 pKa = 10.59ALIASTALVLTAGIAAADD21 pKa = 3.89VTISGYY27 pKa = 10.89GRR29 pKa = 11.84TGVLYY34 pKa = 10.56QEE36 pKa = 4.84GGVRR40 pKa = 11.84GPIDD44 pKa = 3.64TNGDD48 pKa = 3.51APGGLVDD55 pKa = 4.87ASDD58 pKa = 5.51AIVQSRR64 pKa = 11.84LRR66 pKa = 11.84MNIDD70 pKa = 2.93ATTSTDD76 pKa = 3.03QGVDD80 pKa = 3.57FGGRR84 pKa = 11.84IRR86 pKa = 11.84IQWDD90 pKa = 3.02QGDD93 pKa = 4.56DD94 pKa = 3.65EE95 pKa = 4.85TTVAPGYY102 pKa = 10.73VYY104 pKa = 9.67VTASGLTVEE113 pKa = 5.0IGNSNTAYY121 pKa = 10.68DD122 pKa = 4.06SAALLYY128 pKa = 10.25NSEE131 pKa = 3.83IGVYY135 pKa = 9.93SRR137 pKa = 11.84SFGNSRR143 pKa = 11.84GDD145 pKa = 3.32FFAYY149 pKa = 8.89NTDD152 pKa = 3.82GYY154 pKa = 8.15PTLANVVDD162 pKa = 4.18GAIDD166 pKa = 3.47RR167 pKa = 11.84RR168 pKa = 11.84SDD170 pKa = 3.35YY171 pKa = 10.08MGVMVKK177 pKa = 10.39YY178 pKa = 10.29AVAGVNLRR186 pKa = 11.84ASVIDD191 pKa = 3.77PDD193 pKa = 3.9QVYY196 pKa = 10.72DD197 pKa = 4.13LPTDD201 pKa = 3.64TSEE204 pKa = 4.48KK205 pKa = 9.34EE206 pKa = 4.02YY207 pKa = 10.96SVSADD212 pKa = 3.61YY213 pKa = 11.03VWNDD217 pKa = 3.07SVTLSAAYY225 pKa = 9.62VKK227 pKa = 10.72NGAGIDD233 pKa = 4.31DD234 pKa = 3.91NDD236 pKa = 3.6QYY238 pKa = 11.76FVGAEE243 pKa = 3.72YY244 pKa = 11.08AFNDD248 pKa = 3.54KK249 pKa = 10.56TDD251 pKa = 3.0IGLLYY256 pKa = 9.8IDD258 pKa = 4.27NGEE261 pKa = 4.19VLGDD265 pKa = 4.07DD266 pKa = 3.7SDD268 pKa = 4.03LGKK271 pKa = 9.27TITLYY276 pKa = 11.02GSYY279 pKa = 9.9EE280 pKa = 4.09VAPLTTINGYY290 pKa = 8.14VANNSADD297 pKa = 3.74SNEE300 pKa = 3.89SDD302 pKa = 3.24NAFGIGGAYY311 pKa = 10.22DD312 pKa = 3.52LGGAMLAGSVQRR324 pKa = 11.84GYY326 pKa = 11.26DD327 pKa = 3.56EE328 pKa = 6.24NITADD333 pKa = 3.29MGVQFNFF340 pKa = 3.6

Molecular weight:
35.94 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0D6TE65|A0A0D6TE65_9RHOB ABC transporter substrate-binding protein OS=Paracoccus sp. 228 OX=1192054 GN=SY26_06145 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.37GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.39GGRR28 pKa = 11.84LVLNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.7GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight:
5.16 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3463

0

3463

1070115

28

2108

309.0

33.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.467 ± 0.067

0.803 ± 0.011

6.449 ± 0.04

4.904 ± 0.044

3.402 ± 0.027

8.861 ± 0.045

2.106 ± 0.021

5.035 ± 0.029

2.288 ± 0.036

10.372 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.882 ± 0.02

2.206 ± 0.021

5.421 ± 0.028

3.53 ± 0.023

7.513 ± 0.043

4.674 ± 0.027

5.434 ± 0.028

7.257 ± 0.036

1.457 ± 0.02

1.933 ± 0.021

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski