Rhodococcus sp. CUA-806

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Corynebacteriales; Nocardiaceae; Rhodococcus; unclassified Rhodococcus

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3844 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q9U6Q6|A0A1Q9U6Q6_9NOCA Uncharacterized protein OS=Rhodococcus sp. CUA-806 OX=1517936 GN=BJF84_22975 PE=4 SV=1
MM1 pKa = 7.44VEE3 pKa = 4.25NEE5 pKa = 4.02TPPRR9 pKa = 11.84HH10 pKa = 6.04DD11 pKa = 3.68AVMTALVIVSIVATGIIFAGLTATPEE37 pKa = 4.15EE38 pKa = 4.77PEE40 pKa = 4.39LTVQPTTTTPLPPPPQEE57 pKa = 3.74EE58 pKa = 4.86EE59 pKa = 4.56YY60 pKa = 10.82ILVTPEE66 pKa = 3.3VAYY69 pKa = 8.44PTEE72 pKa = 4.16IPGCDD77 pKa = 3.29SVEE80 pKa = 4.21EE81 pKa = 4.1PAEE84 pKa = 4.01PVYY87 pKa = 10.75SSYY90 pKa = 10.89MSDD93 pKa = 3.24GLASYY98 pKa = 10.18DD99 pKa = 3.79NPIAPWFSGPKK110 pKa = 8.74AHH112 pKa = 6.97LMSKK116 pKa = 10.62ALLASLPSDD125 pKa = 4.59LEE127 pKa = 4.3FEE129 pKa = 5.07DD130 pKa = 5.17GRR132 pKa = 11.84IPYY135 pKa = 9.41FDD137 pKa = 6.31PIPVYY142 pKa = 10.66EE143 pKa = 4.95DD144 pKa = 3.35ADD146 pKa = 3.84FTIDD150 pKa = 3.29STNAYY155 pKa = 7.65GTIGLDD161 pKa = 3.48DD162 pKa = 4.34GSGYY166 pKa = 10.7LSVGVAQFSEE176 pKa = 4.99EE177 pKa = 4.32IPPCLAGTLDD187 pKa = 3.55EE188 pKa = 5.92RR189 pKa = 11.84RR190 pKa = 11.84TQSDD194 pKa = 3.69GTVVDD199 pKa = 4.82IHH201 pKa = 5.62TTWQEE206 pKa = 3.96VNGEE210 pKa = 4.16RR211 pKa = 11.84TSTRR215 pKa = 11.84SAIAYY220 pKa = 7.56LTDD223 pKa = 4.04GSQVSAYY230 pKa = 9.12TSAIGDD236 pKa = 3.69ADD238 pKa = 4.15AVPLSVDD245 pKa = 3.21EE246 pKa = 4.39LVRR249 pKa = 11.84IVTYY253 pKa = 10.32PGLDD257 pKa = 3.29TSTAPPPGTPGNSMEE272 pKa = 4.2CTPYY276 pKa = 10.16GTDD279 pKa = 3.24TTRR282 pKa = 11.84KK283 pKa = 9.6LSPDD287 pKa = 4.23DD288 pKa = 4.18IEE290 pKa = 4.45TLNRR294 pKa = 11.84ALQQAATGGLAPSPPLGALRR314 pKa = 11.84TSSWGDD320 pKa = 3.33GLCQVVNSAAGALTVSVGGAPKK342 pKa = 9.9PDD344 pKa = 4.23APDD347 pKa = 4.15PDD349 pKa = 4.17APVPVSGPYY358 pKa = 10.45GGGTTISVPTPSGLGITVTTDD379 pKa = 4.14DD380 pKa = 3.75SWDD383 pKa = 3.52PANLEE388 pKa = 4.56RR389 pKa = 11.84IAMTPGLDD397 pKa = 3.22IPP399 pKa = 4.61

Molecular weight: 41.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q9UCV0|A0A1Q9UCV0_9NOCA 3-hydroxybutyrate dehydrogenase OS=Rhodococcus sp. CUA-806 OX=1517936 GN=BJF84_14765 PE=4 SV=1
MM1 pKa = 7.51SRR3 pKa = 11.84PLTDD7 pKa = 3.29AATSARR13 pKa = 11.84LSKK16 pKa = 10.08QRR18 pKa = 11.84RR19 pKa = 11.84RR20 pKa = 11.84DD21 pKa = 3.69TKK23 pKa = 10.6PEE25 pKa = 3.52VALRR29 pKa = 11.84RR30 pKa = 11.84EE31 pKa = 3.88LHH33 pKa = 6.02RR34 pKa = 11.84RR35 pKa = 11.84GLRR38 pKa = 11.84YY39 pKa = 9.24FVDD42 pKa = 3.95RR43 pKa = 11.84APLKK47 pKa = 10.11GMRR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84ADD54 pKa = 3.13VVFPRR59 pKa = 11.84RR60 pKa = 11.84KK61 pKa = 8.27VAVYY65 pKa = 10.57VDD67 pKa = 3.33GCFWHH72 pKa = 6.75SCPIHH77 pKa = 5.61ATQPRR82 pKa = 11.84NNAQWWADD90 pKa = 3.42KK91 pKa = 10.98LSANVARR98 pKa = 11.84DD99 pKa = 3.3RR100 pKa = 11.84NTDD103 pKa = 2.98EE104 pKa = 4.67RR105 pKa = 11.84LEE107 pKa = 4.33AEE109 pKa = 4.03GWTVVRR115 pKa = 11.84VWEE118 pKa = 4.48HH119 pKa = 5.94EE120 pKa = 4.1PAADD124 pKa = 3.53AAEE127 pKa = 4.39KK128 pKa = 10.16VAQALGG134 pKa = 3.42

Molecular weight: 15.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3844

0

3844

1045091

30

2113

271.9

29.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.321 ± 0.06

0.776 ± 0.011

6.321 ± 0.037

5.567 ± 0.038

3.101 ± 0.024

8.511 ± 0.036

2.189 ± 0.024

4.532 ± 0.027

2.214 ± 0.027

9.776 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.056 ± 0.015

2.174 ± 0.02

5.349 ± 0.03

2.793 ± 0.022

7.155 ± 0.045

6.442 ± 0.03

6.351 ± 0.03

8.877 ± 0.038

1.452 ± 0.018

2.044 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski