Gordonia phage GMA7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Getseptimavirus; Gordonia virus GMA7

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 101 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0K0N777|A0A0K0N777_9CAUD Uncharacterized protein OS=Gordonia phage GMA7 OX=1647286 GN=GMA7_69 PE=4 SV=1
MM1 pKa = 7.64HH2 pKa = 7.59GPPYY6 pKa = 10.63YY7 pKa = 10.09YY8 pKa = 10.63GPPDD12 pKa = 3.7WPEE15 pKa = 3.44PHH17 pKa = 6.95PWEE20 pKa = 4.39EE21 pKa = 3.69ALQYY25 pKa = 11.3VPFDD29 pKa = 3.49VPLTDD34 pKa = 3.61EE35 pKa = 4.83EE36 pKa = 5.02KK37 pKa = 11.12EE38 pKa = 4.09EE39 pKa = 4.48LPPDD43 pKa = 4.33PEE45 pKa = 5.06DD46 pKa = 3.66EE47 pKa = 4.31PP48 pKa = 5.54

Molecular weight: 5.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0K0N6H9|A0A0K0N6H9_9CAUD Uncharacterized protein OS=Gordonia phage GMA7 OX=1647286 GN=GMA7_98 PE=4 SV=1
MM1 pKa = 7.36VGTINTIASLTGHH14 pKa = 6.33SFLHH18 pKa = 6.12CLCGTTYY25 pKa = 11.17DD26 pKa = 3.55LRR28 pKa = 11.84IFFWYY33 pKa = 9.99RR34 pKa = 11.84GLSRR38 pKa = 11.84EE39 pKa = 4.13LFSTRR44 pKa = 11.84RR45 pKa = 11.84VDD47 pKa = 3.51QTLLPMSSPNCWPLYY62 pKa = 9.76GPYY65 pKa = 9.1ATRR68 pKa = 11.84VPNRR72 pKa = 11.84LHH74 pKa = 5.93STGGPNSARR83 pKa = 11.84QRR85 pKa = 11.84CNQRR89 pKa = 11.84GVIAKK94 pKa = 9.59DD95 pKa = 3.19GCHH98 pKa = 4.18YY99 pKa = 9.33TGPHH103 pKa = 5.98FEE105 pKa = 4.09STKK108 pKa = 8.95QVRR111 pKa = 11.84QPSNEE116 pKa = 3.61RR117 pKa = 11.84FALARR122 pKa = 11.84AEE124 pKa = 4.34LFAKK128 pKa = 10.39SSRR131 pKa = 11.84LLNRR135 pKa = 11.84SHH137 pKa = 6.85EE138 pKa = 4.28MFRR141 pKa = 11.84VWGG144 pKa = 3.8

Molecular weight: 16.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

101

0

101

22794

37

3046

225.7

24.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.844 ± 0.764

0.877 ± 0.147

6.238 ± 0.252

6.954 ± 0.476

3.527 ± 0.136

7.835 ± 0.409

1.693 ± 0.174

5.488 ± 0.165

5.37 ± 0.268

7.612 ± 0.319

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.874 ± 0.159

4.212 ± 0.17

4.914 ± 0.324

3.584 ± 0.15

5.418 ± 0.396

6.62 ± 0.221

6.278 ± 0.307

6.945 ± 0.201

1.755 ± 0.181

2.961 ± 0.237
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski