Xinzhou nematode virus 4

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Alphanemrhavirus; Xinzhou alphanemrhavirus

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KNA4|A0A1L3KNA4_9RHAB GDP polyribonucleotidyltransferase OS=Xinzhou nematode virus 4 OX=1923772 PE=4 SV=1
MM1 pKa = 7.69SDD3 pKa = 3.54SNEE6 pKa = 4.41SPPEE10 pKa = 3.75HH11 pKa = 6.32QFAVPAVDD19 pKa = 3.59HH20 pKa = 6.08GHH22 pKa = 7.3LARR25 pKa = 11.84LQRR28 pKa = 11.84EE29 pKa = 3.93INLRR33 pKa = 11.84ANIAQRR39 pKa = 11.84DD40 pKa = 3.87STLFRR45 pKa = 11.84HH46 pKa = 6.33VEE48 pKa = 3.98LTDD51 pKa = 4.15DD52 pKa = 3.99SPVIPTQQVFEE63 pKa = 4.68RR64 pKa = 11.84PPKK67 pKa = 10.46LSTSKK72 pKa = 8.99VTRR75 pKa = 11.84EE76 pKa = 4.2EE77 pKa = 3.87IQHH80 pKa = 6.12ARR82 pKa = 11.84TMSIISNEE90 pKa = 3.96SSKK93 pKa = 9.92TGSEE97 pKa = 4.02QQLTFPIPSTDD108 pKa = 3.88CDD110 pKa = 3.6AEE112 pKa = 4.5SYY114 pKa = 10.42IAGIHH119 pKa = 5.56AACIEE124 pKa = 4.35LTSIMKK130 pKa = 9.59FKK132 pKa = 10.41IDD134 pKa = 3.5YY135 pKa = 10.05FDD137 pKa = 5.08SNEE140 pKa = 3.71STAIVGISKK149 pKa = 8.01MHH151 pKa = 5.98SLSRR155 pKa = 11.84STSSTQMSTEE165 pKa = 4.15PLSVAPPSTQSSHH178 pKa = 5.53MDD180 pKa = 3.01VSGTEE185 pKa = 4.15EE186 pKa = 3.99PLSLNEE192 pKa = 3.45ILARR196 pKa = 11.84YY197 pKa = 8.21DD198 pKa = 3.81DD199 pKa = 4.46PVEE202 pKa = 4.09LTDD205 pKa = 4.23NLGEE209 pKa = 4.07MFQVYY214 pKa = 7.44WKK216 pKa = 11.12NEE218 pKa = 3.29ISHH221 pKa = 6.53PKK223 pKa = 9.56EE224 pKa = 3.65LLAQVIKK231 pKa = 10.86KK232 pKa = 9.64IGKK235 pKa = 9.12EE236 pKa = 3.8KK237 pKa = 10.68FLDD240 pKa = 3.77RR241 pKa = 11.84PLNQQVCLMILGDD254 pKa = 3.89PSFEE258 pKa = 4.01YY259 pKa = 10.78LRR261 pKa = 11.84GIVAPSFRR269 pKa = 11.84RR270 pKa = 3.47

Molecular weight: 30.26 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KNH6|A0A1L3KNH6_9RHAB Uncharacterized protein OS=Xinzhou nematode virus 4 OX=1923772 PE=4 SV=1
MM1 pKa = 7.8DD2 pKa = 3.69VSAIKK7 pKa = 10.69VLLAVLSVVHH17 pKa = 6.89PILIPDD23 pKa = 4.28CKK25 pKa = 11.02SNTVWFPATLDD36 pKa = 3.14ISMCPLPLTEE46 pKa = 4.13YY47 pKa = 9.49TSSIFKK53 pKa = 10.07EE54 pKa = 4.25GQIGLFSPEE63 pKa = 5.05DD64 pKa = 3.56MTQKK68 pKa = 10.78LVDD71 pKa = 3.63GAICYY76 pKa = 10.23IKK78 pKa = 10.49VSKK81 pKa = 10.25VKK83 pKa = 9.72CTTGFFWSQNIQKK96 pKa = 9.57SVSFRR101 pKa = 11.84RR102 pKa = 11.84PPKK105 pKa = 9.62SACRR109 pKa = 11.84DD110 pKa = 3.72HH111 pKa = 7.62IDD113 pKa = 3.36QQRR116 pKa = 11.84EE117 pKa = 4.07EE118 pKa = 4.09ISFTEE123 pKa = 4.4AEE125 pKa = 4.71FPTPVCKK132 pKa = 10.28FQLLSSTTTTTEE144 pKa = 3.77SEE146 pKa = 4.31TLIVEE151 pKa = 4.1PHH153 pKa = 6.75PIEE156 pKa = 4.11YY157 pKa = 10.16SYY159 pKa = 11.45RR160 pKa = 11.84DD161 pKa = 3.52QLLLNSVLLRR171 pKa = 11.84GKK173 pKa = 9.89CRR175 pKa = 11.84EE176 pKa = 4.36RR177 pKa = 11.84ICPTYY182 pKa = 10.47RR183 pKa = 11.84LEE185 pKa = 4.7AYY187 pKa = 8.86WIAKK191 pKa = 9.98DD192 pKa = 4.36PLPDD196 pKa = 4.17CPPMTRR202 pKa = 11.84HH203 pKa = 5.85PAKK206 pKa = 10.27IYY208 pKa = 10.57FDD210 pKa = 3.6GQKK213 pKa = 9.25PVSVISDD220 pKa = 3.46VLPPLGLKK228 pKa = 10.04GSCRR232 pKa = 11.84TVYY235 pKa = 10.26CGQPGIRR242 pKa = 11.84TVEE245 pKa = 3.97NIFINISGVDD255 pKa = 3.44ITSQIPTCTEE265 pKa = 3.49RR266 pKa = 11.84HH267 pKa = 4.44QLEE270 pKa = 4.82GFSKK274 pKa = 10.72DD275 pKa = 2.99ISSKK279 pKa = 11.3GEE281 pKa = 3.74LLTFEE286 pKa = 4.58EE287 pKa = 4.52HH288 pKa = 6.15TKK290 pKa = 9.31YY291 pKa = 9.21TLCIYY296 pKa = 10.79AVILMKK302 pKa = 10.61SLEE305 pKa = 4.3VKK307 pKa = 10.34HH308 pKa = 6.63PYY310 pKa = 9.74LLSVFQPQFAGSHH323 pKa = 5.21PCYY326 pKa = 10.21RR327 pKa = 11.84IFNRR331 pKa = 11.84TTIMKK336 pKa = 9.19AICPYY341 pKa = 10.45IHH343 pKa = 7.11MDD345 pKa = 3.46PVGVSPHH352 pKa = 5.68GVIGTDD358 pKa = 2.95KK359 pKa = 10.65HH360 pKa = 6.49SKK362 pKa = 8.29PVIWKK367 pKa = 9.36FWDD370 pKa = 3.3ALTKK374 pKa = 9.86EE375 pKa = 4.63KK376 pKa = 10.89CPSLGPNGIIRR387 pKa = 11.84DD388 pKa = 4.08EE389 pKa = 4.17QCKK392 pKa = 8.04VHH394 pKa = 6.31YY395 pKa = 8.59PHH397 pKa = 7.48EE398 pKa = 4.48YY399 pKa = 9.14VGSDD403 pKa = 2.57IMSLIGEE410 pKa = 4.56MMRR413 pKa = 11.84DD414 pKa = 3.26HH415 pKa = 7.22APIEE419 pKa = 4.0KK420 pKa = 10.54SFDD423 pKa = 2.83ILTRR427 pKa = 11.84NTQSGNVEE435 pKa = 3.55ISDD438 pKa = 3.71KK439 pKa = 10.93RR440 pKa = 11.84KK441 pKa = 9.23SQSDD445 pKa = 3.36SRR447 pKa = 11.84SFIDD451 pKa = 5.08AMSDD455 pKa = 3.75DD456 pKa = 4.18FTTLKK461 pKa = 10.77ADD463 pKa = 3.56FLIAVKK469 pKa = 10.4AFAGFCVVCVLYY481 pKa = 10.61KK482 pKa = 10.91LKK484 pKa = 10.5FFKK487 pKa = 10.95LITKK491 pKa = 7.43MLCCCISLGKK501 pKa = 10.57LSTKK505 pKa = 10.53LFVASSSTVLRR516 pKa = 11.84SSNTHH521 pKa = 5.39RR522 pKa = 11.84LRR524 pKa = 11.84RR525 pKa = 11.84RR526 pKa = 11.84QRR528 pKa = 11.84RR529 pKa = 11.84HH530 pKa = 5.35EE531 pKa = 4.56EE532 pKa = 3.5IEE534 pKa = 4.12MNPFAA539 pKa = 6.55

Molecular weight: 61.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

3647

218

2184

729.4

82.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.744 ± 1.103

2.029 ± 0.449

4.469 ± 0.206

6.115 ± 0.292

4.689 ± 0.37

5.265 ± 0.321

2.934 ± 0.157

7.211 ± 0.599

5.95 ± 0.348

11.078 ± 0.959

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.358 ± 0.105

4.442 ± 0.744

5.1 ± 0.574

3.427 ± 0.224

4.415 ± 0.276

9.186 ± 0.54

5.868 ± 0.352

5.703 ± 0.508

1.508 ± 0.359

3.51 ± 0.399
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski