Bosea lathyri

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Boseaceae; Bosea

Average proteome isoelectric point is 6.84

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5472 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H5XJ11|A0A1H5XJ11_9BRAD Enoyl-(Acyl carrier protein) reductase OS=Bosea lathyri OX=1036778 GN=SAMN04488115_103269 PE=4 SV=1
MM1 pKa = 7.3TSSLSEE7 pKa = 4.05ASPLSDD13 pKa = 4.91LQNSWPRR20 pKa = 11.84EE21 pKa = 4.07LYY23 pKa = 10.2RR24 pKa = 11.84EE25 pKa = 4.25HH26 pKa = 6.91CQDD29 pKa = 3.49EE30 pKa = 4.48EE31 pKa = 4.56GNPYY35 pKa = 10.02VVIVWQEE42 pKa = 3.77RR43 pKa = 11.84PGLGITTYY51 pKa = 9.29TLEE54 pKa = 4.94DD55 pKa = 4.22GSPVTYY61 pKa = 10.57EE62 pKa = 4.66DD63 pKa = 3.84GCDD66 pKa = 3.74FQIVATGKK74 pKa = 10.54FITRR78 pKa = 11.84CEE80 pKa = 4.18DD81 pKa = 3.36PDD83 pKa = 4.97AEE85 pKa = 4.41TT86 pKa = 4.36

Molecular weight: 9.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H5Z6R0|A0A1H5Z6R0_9BRAD Uncharacterized protein OS=Bosea lathyri OX=1036778 GN=SAMN04488115_104294 PE=4 SV=1
MM1 pKa = 7.54IPVEE5 pKa = 3.89QDD7 pKa = 2.98GPPEE11 pKa = 3.92HH12 pKa = 7.07KK13 pKa = 10.44FPFRR17 pKa = 11.84ALPFRR22 pKa = 11.84AARR25 pKa = 11.84WSAAALRR32 pKa = 11.84SGLRR36 pKa = 11.84GVIGIVYY43 pKa = 9.11PPSCIACQAATGEE56 pKa = 4.15AQALCATCWGDD67 pKa = 2.56IGFIEE72 pKa = 4.63RR73 pKa = 11.84PYY75 pKa = 10.65CEE77 pKa = 4.4RR78 pKa = 11.84LGTPFAVDD86 pKa = 3.97LGAGLLSPAAIADD99 pKa = 3.7PPVFTRR105 pKa = 11.84ARR107 pKa = 11.84AVCRR111 pKa = 11.84FDD113 pKa = 3.0GTARR117 pKa = 11.84EE118 pKa = 4.06LVHH121 pKa = 6.32RR122 pKa = 11.84LKK124 pKa = 11.28YY125 pKa = 10.07GDD127 pKa = 3.76RR128 pKa = 11.84VEE130 pKa = 5.51LGLTLGRR137 pKa = 11.84MMAQAGRR144 pKa = 11.84EE145 pKa = 4.05LLGDD149 pKa = 3.91ADD151 pKa = 5.39LIVPVPLHH159 pKa = 5.68RR160 pKa = 11.84TRR162 pKa = 11.84LWSRR166 pKa = 11.84RR167 pKa = 11.84FNQAAVLARR176 pKa = 11.84VVSNVSGVALSPLALARR193 pKa = 11.84VKK195 pKa = 9.91RR196 pKa = 11.84TRR198 pKa = 11.84QQVGLTRR205 pKa = 11.84PQRR208 pKa = 11.84ADD210 pKa = 3.14NLQGAFKK217 pKa = 10.55VPAGAKK223 pKa = 8.86PQIEE227 pKa = 4.34GRR229 pKa = 11.84RR230 pKa = 11.84ILLIDD235 pKa = 4.25DD236 pKa = 3.97VLTTGSTANAAARR249 pKa = 11.84ALLRR253 pKa = 11.84AGARR257 pKa = 11.84DD258 pKa = 3.32VDD260 pKa = 3.91MLTFARR266 pKa = 11.84VVTDD270 pKa = 3.46AA271 pKa = 4.72

Molecular weight: 29.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5472

0

5472

1726800

39

3064

315.6

34.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.094 ± 0.04

0.799 ± 0.011

5.353 ± 0.029

5.401 ± 0.029

3.734 ± 0.02

8.833 ± 0.04

1.918 ± 0.015

5.335 ± 0.019

3.222 ± 0.027

10.433 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.415 ± 0.015

2.419 ± 0.019

5.311 ± 0.026

3.055 ± 0.021

7.135 ± 0.039

5.475 ± 0.023

5.256 ± 0.022

7.437 ± 0.024

1.299 ± 0.013

2.076 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski