Mesorhizobium phage Cp1R7A-A1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 210 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6H0QV40|A0A6H0QV40_9CAUD Uncharacterized protein OS=Mesorhizobium phage Cp1R7A-A1 OX=2723894 GN=Cp1R7AA1_137 PE=4 SV=1
MM1 pKa = 7.23PKK3 pKa = 10.15KK4 pKa = 10.68YY5 pKa = 10.74VVDD8 pKa = 3.73QDD10 pKa = 4.74DD11 pKa = 4.06LPEE14 pKa = 4.3TEE16 pKa = 4.35PTEE19 pKa = 4.24SCAMCGKK26 pKa = 10.42DD27 pKa = 2.88IFTGPFCEE35 pKa = 4.37EE36 pKa = 4.69CDD38 pKa = 3.74TEE40 pKa = 4.5TSFDD44 pKa = 3.58NSLEE48 pKa = 4.08EE49 pKa = 4.37DD50 pKa = 2.87WDD52 pKa = 4.01AFGDD56 pKa = 4.22DD57 pKa = 4.2DD58 pKa = 4.84PKK60 pKa = 11.41DD61 pKa = 3.64RR62 pKa = 11.84SFLDD66 pKa = 3.86DD67 pKa = 4.62GYY69 pKa = 11.48HH70 pKa = 6.31LL71 pKa = 4.67

Molecular weight: 8.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6H0QVA5|A0A6H0QVA5_9CAUD Uncharacterized protein OS=Mesorhizobium phage Cp1R7A-A1 OX=2723894 GN=Cp1R7AA1_194 PE=4 SV=1
MM1 pKa = 7.81KK2 pKa = 10.56LPIAKK7 pKa = 8.88TEE9 pKa = 3.86IAKK12 pKa = 10.04KK13 pKa = 9.86RR14 pKa = 11.84RR15 pKa = 11.84PGNGLLPGLRR25 pKa = 11.84KK26 pKa = 9.15LAKK29 pKa = 10.09ADD31 pKa = 3.42IDD33 pKa = 4.42SVDD36 pKa = 3.73LTVSAFLPIDD46 pKa = 3.79FVPLSGRR53 pKa = 11.84LRR55 pKa = 11.84IACRR59 pKa = 11.84CSHH62 pKa = 6.69GDD64 pKa = 3.31LPRR67 pKa = 11.84YY68 pKa = 10.02DD69 pKa = 3.94FLARR73 pKa = 11.84ARR75 pKa = 11.84RR76 pKa = 11.84EE77 pKa = 3.88EE78 pKa = 3.68IRR80 pKa = 11.84FSVAQAMPARR90 pKa = 11.84EE91 pKa = 3.82IAFASALSAPAHH103 pKa = 6.57AARR106 pKa = 11.84PMM108 pKa = 3.63

Molecular weight: 11.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

210

0

210

38144

34

1678

181.6

20.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.007 ± 0.278

0.946 ± 0.079

6.683 ± 0.166

6.234 ± 0.269

3.875 ± 0.121

7.676 ± 0.265

2.076 ± 0.121

5.249 ± 0.116

5.406 ± 0.175

7.925 ± 0.15

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.792 ± 0.115

3.61 ± 0.165

4.743 ± 0.16

3.204 ± 0.143

6.051 ± 0.252

5.76 ± 0.158

6.268 ± 0.338

6.958 ± 0.173

1.775 ± 0.099

2.763 ± 0.101
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski