Mycobacterium phage SWU2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VHZ6|A0A2K9VHZ6_9CAUD HNH endonuclease OS=Mycobacterium phage SWU2 OX=2077150 GN=JX_gp01 PE=4 SV=1
MM1 pKa = 7.83LLRR4 pKa = 11.84GFAPTGDD11 pKa = 3.57PTGALGQHH19 pKa = 6.34GGLLGVIPGPPGPKK33 pKa = 9.74GDD35 pKa = 3.69GLEE38 pKa = 3.99IDD40 pKa = 4.72GVYY43 pKa = 10.77ADD45 pKa = 4.02YY46 pKa = 11.61SEE48 pKa = 5.01FPSDD52 pKa = 5.37LGPEE56 pKa = 4.22DD57 pKa = 3.53EE58 pKa = 5.63GFAAFNQYY66 pKa = 10.69DD67 pKa = 3.76GQLYY71 pKa = 8.26MWSGTAWPLEE81 pKa = 3.96GTGAQFQGEE90 pKa = 4.7VGPQGPVGPVGPQGPVGPQGPQGIQGPTGPEE121 pKa = 4.35GPEE124 pKa = 4.16GPQGNGIQIDD134 pKa = 4.34GQVATYY140 pKa = 11.12AEE142 pKa = 4.57LPDD145 pKa = 3.72TGVDD149 pKa = 4.09EE150 pKa = 4.99GDD152 pKa = 2.97VWLAATKK159 pKa = 10.26LYY161 pKa = 10.43RR162 pKa = 11.84YY163 pKa = 9.94DD164 pKa = 3.38GSAWPAEE171 pKa = 4.19EE172 pKa = 5.66DD173 pKa = 3.7GAPVQGPQGPQGEE186 pKa = 4.5TGEE189 pKa = 4.45TGPEE193 pKa = 4.44GPVGPAGPTEE203 pKa = 3.78WLEE206 pKa = 3.57ITNRR210 pKa = 11.84PKK212 pKa = 10.45VAYY215 pKa = 9.77AVLDD219 pKa = 4.13GVATDD224 pKa = 3.02ITMWFGSEE232 pKa = 3.52AAYY235 pKa = 8.58NTITPKK241 pKa = 10.82NPTTLYY247 pKa = 10.38HH248 pKa = 7.16RR249 pKa = 11.84YY250 pKa = 9.61

Molecular weight: 25.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VI09|A0A2K9VI09_9CAUD Uncharacterized protein OS=Mycobacterium phage SWU2 OX=2077150 GN=JX_gp37 PE=4 SV=1
MM1 pKa = 7.51PVDD4 pKa = 3.4SATFAEE10 pKa = 4.45MRR12 pKa = 11.84GGQEE16 pKa = 3.52FRR18 pKa = 11.84GWDD21 pKa = 3.28EE22 pKa = 4.97DD23 pKa = 4.49RR24 pKa = 11.84YY25 pKa = 10.89QGTALVDD32 pKa = 3.37ATRR35 pKa = 11.84GLLYY39 pKa = 10.9AFILAHH45 pKa = 5.77TGKK48 pKa = 9.44NQRR51 pKa = 11.84KK52 pKa = 6.46PTAPTPWPIPDD63 pKa = 3.22RR64 pKa = 11.84KK65 pKa = 9.92HH66 pKa = 5.3RR67 pKa = 11.84QEE69 pKa = 4.01RR70 pKa = 11.84QKK72 pKa = 9.65NTPGSFYY79 pKa = 11.1RR80 pKa = 11.84MVASQVARR88 pKa = 11.84KK89 pKa = 9.34RR90 pKa = 11.84KK91 pKa = 9.32QKK93 pKa = 10.98GGGG96 pKa = 3.3

Molecular weight: 10.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

15598

37

1125

205.2

22.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.411 ± 0.365

0.814 ± 0.118

6.687 ± 0.262

6.405 ± 0.302

3.315 ± 0.177

9.149 ± 0.511

1.917 ± 0.209

4.738 ± 0.153

4.257 ± 0.184

8.315 ± 0.441

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.391 ± 0.129

3.18 ± 0.152

5.783 ± 0.29

3.859 ± 0.269

6.206 ± 0.328

5.308 ± 0.183

6.187 ± 0.282

7.443 ± 0.221

1.872 ± 0.182

2.763 ± 0.184
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski