Hirschia baltica (strain ATCC 49814 / DSM 5838 / IFAM 1418)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomonadales; Hyphomonadaceae; Hirschia; Hirschia baltica

Average proteome isoelectric point is 5.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3187 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6XQF2|C6XQF2_HIRBI Uroporphyrinogen III methylase OS=Hirschia baltica (strain ATCC 49814 / DSM 5838 / IFAM 1418) OX=582402 GN=Hbal_2778 PE=3 SV=1
MM1 pKa = 7.58IMTAPAIFVRR11 pKa = 11.84KK12 pKa = 9.21NIKK15 pKa = 8.74RR16 pKa = 11.84TSPLVFLTILYY27 pKa = 7.52STCSPLALGQEE38 pKa = 4.32ISSATSTPVEE48 pKa = 4.37TATLDD53 pKa = 3.37NGSASDD59 pKa = 4.4LTITDD64 pKa = 4.3DD65 pKa = 6.26GSIEE69 pKa = 4.01WDD71 pKa = 3.58SVDD74 pKa = 3.72DD75 pKa = 3.98QVAVTVNSSNNVTIDD90 pKa = 3.6GDD92 pKa = 3.75ITLEE96 pKa = 4.06NSDD99 pKa = 3.85NNVGVKK105 pKa = 10.07INPNLSSTINLSGAINLIEE124 pKa = 5.14DD125 pKa = 3.95YY126 pKa = 11.35ARR128 pKa = 11.84TDD130 pKa = 3.15TDD132 pKa = 5.5DD133 pKa = 6.3DD134 pKa = 5.04DD135 pKa = 6.64DD136 pKa = 6.67LDD138 pKa = 3.99GLIAEE143 pKa = 4.62GTNRR147 pKa = 11.84HH148 pKa = 6.04GIFLEE153 pKa = 4.21SGGINTGDD161 pKa = 4.47IILQSGSSIYY171 pKa = 10.8VEE173 pKa = 4.88GNNSTAITLQSGLNGNYY190 pKa = 9.39IQDD193 pKa = 3.58GSISVYY199 pKa = 10.2GANSTALDD207 pKa = 3.37IQNDD211 pKa = 3.47VNGNVRR217 pKa = 11.84VSGGVTANGEE227 pKa = 4.0NASAISIAGDD237 pKa = 2.99ITGNLTLEE245 pKa = 4.11NTITSTGFTSTTSSNYY261 pKa = 9.69IGPDD265 pKa = 4.27DD266 pKa = 5.4IDD268 pKa = 6.07DD269 pKa = 4.1DD270 pKa = 4.25TAALEE275 pKa = 4.64DD276 pKa = 4.46RR277 pKa = 11.84LDD279 pKa = 3.94NDD281 pKa = 4.78DD282 pKa = 4.98LFNSASSVSIGGSIANGLLINGYY305 pKa = 9.62HH306 pKa = 7.46DD307 pKa = 4.03YY308 pKa = 11.29VDD310 pKa = 5.1DD311 pKa = 5.85GIAVDD316 pKa = 5.33DD317 pKa = 5.51DD318 pKa = 3.99EE319 pKa = 5.06TKK321 pKa = 10.44DD322 pKa = 3.82TIDD325 pKa = 4.86DD326 pKa = 3.87YY327 pKa = 11.98NEE329 pKa = 3.77NRR331 pKa = 11.84GTGYY335 pKa = 10.11ISTYY339 pKa = 10.06GSSPALQITPDD350 pKa = 4.51LIIGSSSDD358 pKa = 3.52LVLGSVVEE366 pKa = 4.45TVRR369 pKa = 11.84DD370 pKa = 3.74TLDD373 pKa = 3.79DD374 pKa = 5.81DD375 pKa = 4.6EE376 pKa = 7.45DD377 pKa = 4.48EE378 pKa = 4.75DD379 pKa = 4.55TNEE382 pKa = 3.8VLATFNFDD390 pKa = 3.05QGLVNLGTIYY400 pKa = 11.14ADD402 pKa = 3.53GQNVGFSATALEE414 pKa = 4.49ISGSADD420 pKa = 3.01GNLNTIITNGILNSGTMSTTAFEE443 pKa = 4.92ANATTVNLGQGAIISSITNDD463 pKa = 2.85GSIISNSTGSSGSNSIAIQISNGANLEE490 pKa = 4.38TIEE493 pKa = 3.97NTGYY497 pKa = 8.44ITAKK501 pKa = 9.06ITDD504 pKa = 3.69DD505 pKa = 3.52TGYY508 pKa = 8.59STAILDD514 pKa = 3.71HH515 pKa = 6.88SGTLTSIFNNGYY527 pKa = 8.82ISSYY531 pKa = 10.96YY532 pKa = 10.47VDD534 pKa = 5.57DD535 pKa = 6.09DD536 pKa = 5.33SDD538 pKa = 4.35DD539 pKa = 5.42DD540 pKa = 5.45EE541 pKa = 7.11YY542 pKa = 11.77DD543 pKa = 4.16DD544 pKa = 4.66NDD546 pKa = 3.5NKK548 pKa = 10.39ISEE551 pKa = 4.32FTGRR555 pKa = 11.84SIAIDD560 pKa = 3.76VSQHH564 pKa = 4.68TASQGVTIVQGNTNSTSPSIYY585 pKa = 10.61GDD587 pKa = 3.29ILFGAGNDD595 pKa = 3.69TLDD598 pKa = 3.74IQDD601 pKa = 3.65GTVAGDD607 pKa = 3.52TYY609 pKa = 10.93FGSGTANFYY618 pKa = 11.07LSDD621 pKa = 3.29ATYY624 pKa = 9.58TGDD627 pKa = 3.23VHH629 pKa = 7.81FEE631 pKa = 4.04SEE633 pKa = 4.25GFNFQSTQGQYY644 pKa = 10.53FGNLNFYY651 pKa = 9.93NNSGSAVFQNNSYY664 pKa = 10.76FSGSLLDD671 pKa = 4.27SDD673 pKa = 5.41NIDD676 pKa = 5.0LIIDD680 pKa = 4.03TSTIYY685 pKa = 10.19ISQDD689 pKa = 2.91SPITLNSLNITGASTLGVIISNSEE713 pKa = 4.36HH714 pKa = 7.0IDD716 pKa = 3.42TPYY719 pKa = 9.4ITATNTAIVGDD730 pKa = 4.48SVTLSSQFTNFVGGDD745 pKa = 3.57FSRR748 pKa = 11.84TILSAPSLDD757 pKa = 3.33VDD759 pKa = 3.39IDD761 pKa = 4.29SISFDD766 pKa = 3.41TSGLSWLYY774 pKa = 9.11NAEE777 pKa = 4.3YY778 pKa = 10.45ILDD781 pKa = 3.78QEE783 pKa = 4.6ADD785 pKa = 3.76GNQSISLQFSQKK797 pKa = 8.82TSEE800 pKa = 4.47DD801 pKa = 3.59LGLDD805 pKa = 3.47AQQDD809 pKa = 3.5RR810 pKa = 11.84TFASFIEE817 pKa = 4.71MISNQQNAGSEE828 pKa = 4.39FVKK831 pKa = 9.76ITDD834 pKa = 3.21EE835 pKa = 4.43TTFLEE840 pKa = 4.89AYY842 pKa = 10.0EE843 pKa = 4.8SILPQHH849 pKa = 6.73SDD851 pKa = 2.79VALRR855 pKa = 11.84HH856 pKa = 6.02LDD858 pKa = 3.16AHH860 pKa = 6.23TNTLNSMVSEE870 pKa = 4.34RR871 pKa = 11.84MALLRR876 pKa = 11.84DD877 pKa = 3.69SSNTSNGFWLQEE889 pKa = 4.11LVSQTTVDD897 pKa = 2.98ASEE900 pKa = 4.15NVNAYY905 pKa = 9.77SGRR908 pKa = 11.84GFGFTAGVDD917 pKa = 3.5RR918 pKa = 11.84RR919 pKa = 11.84IGFIDD924 pKa = 3.56SMGLTFSINDD934 pKa = 3.49EE935 pKa = 4.29KK936 pKa = 11.76YY937 pKa = 7.67EE938 pKa = 4.26TTTSAFNEE946 pKa = 4.27TSSTNFALGFYY957 pKa = 9.33IAEE960 pKa = 4.5RR961 pKa = 11.84IGFADD966 pKa = 4.11LQMSAQIGQTNFKK979 pKa = 10.24SEE981 pKa = 4.16RR982 pKa = 11.84EE983 pKa = 4.17VNFEE987 pKa = 4.18GFNSDD992 pKa = 5.54IEE994 pKa = 4.58GDD996 pKa = 3.49WSGLTQAYY1004 pKa = 6.97STQISSPQKK1013 pKa = 10.32LGWLRR1018 pKa = 11.84VTPHH1022 pKa = 6.64IGASYY1027 pKa = 10.79ISLNQDD1033 pKa = 2.65AYY1035 pKa = 10.7EE1036 pKa = 4.12EE1037 pKa = 4.25TASNGFNLAFSEE1049 pKa = 4.53SEE1051 pKa = 4.43SNKK1054 pKa = 8.8LTGSAGVSLGVFWPSNSGRR1073 pKa = 11.84NSFDD1077 pKa = 3.22LSEE1080 pKa = 4.25NQQSNSSLNGWHH1092 pKa = 6.76AAVDD1096 pKa = 3.66LGIRR1100 pKa = 11.84DD1101 pKa = 4.1TLSSTNYY1108 pKa = 9.74DD1109 pKa = 2.87AVANFVGYY1117 pKa = 10.73DD1118 pKa = 3.36SAFNVYY1124 pKa = 10.16SDD1126 pKa = 4.83EE1127 pKa = 4.48IFGQAFSTGISLVGIGDD1144 pKa = 4.06FASFRR1149 pKa = 11.84LSANAEE1155 pKa = 4.04LSEE1158 pKa = 4.19GASIYY1163 pKa = 10.86SGAASLRR1170 pKa = 11.84FKK1172 pKa = 10.96FF1173 pKa = 4.26

Molecular weight: 124.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6XQ63|C6XQ63_HIRBI Histidine kinase OS=Hirschia baltica (strain ATCC 49814 / DSM 5838 / IFAM 1418) OX=582402 GN=Hbal_0886 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 8.96RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.62GFRR19 pKa = 11.84SRR21 pKa = 11.84MATANGQKK29 pKa = 9.93VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84KK40 pKa = 8.79KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3187

0

3187

1051554

30

1641

330.0

36.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.146 ± 0.055

0.884 ± 0.016

6.076 ± 0.035

6.558 ± 0.041

4.087 ± 0.032

7.596 ± 0.043

1.909 ± 0.023

6.253 ± 0.032

4.909 ± 0.037

9.433 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.579 ± 0.02

3.853 ± 0.028

4.424 ± 0.029

3.439 ± 0.024

5.203 ± 0.03

6.685 ± 0.037

5.398 ± 0.031

6.732 ± 0.034

1.281 ± 0.017

2.555 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski