Mycobacterium phage DonSanchon

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bclasvirinae; Pegunavirus; unclassified Pegunavirus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 97 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MAQ8|A0A345MAQ8_9CAUD Uncharacterized protein OS=Mycobacterium phage DonSanchon OX=2283253 GN=61 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84EE4 pKa = 4.11ICQDD8 pKa = 3.48LADD11 pKa = 4.74DD12 pKa = 4.36VFDD15 pKa = 6.55GDD17 pKa = 4.76EE18 pKa = 4.01QWCDD22 pKa = 2.82ITGEE26 pKa = 3.99HH27 pKa = 6.58HH28 pKa = 6.36VCVMTDD34 pKa = 2.65GHH36 pKa = 5.77IARR39 pKa = 11.84RR40 pKa = 11.84AHH42 pKa = 6.31EE43 pKa = 4.68CDD45 pKa = 5.58CGITWW50 pKa = 4.09

Molecular weight: 5.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MAT8|A0A345MAT8_9CAUD Uncharacterized protein OS=Mycobacterium phage DonSanchon OX=2283253 GN=91 PE=4 SV=1
MM1 pKa = 6.61GTKK4 pKa = 10.17AVVQSRR10 pKa = 11.84VINVNQTITSPTGVAKK26 pKa = 10.53ASKK29 pKa = 10.34ARR31 pKa = 11.84EE32 pKa = 3.92TLATARR38 pKa = 11.84EE39 pKa = 3.82ILYY42 pKa = 10.75ASGNMRR48 pKa = 11.84AADD51 pKa = 4.88AITPALHH58 pKa = 7.4DD59 pKa = 4.2LTRR62 pKa = 11.84QIADD66 pKa = 3.5YY67 pKa = 9.98YY68 pKa = 10.42RR69 pKa = 11.84RR70 pKa = 11.84RR71 pKa = 11.84AEE73 pKa = 3.78ADD75 pKa = 2.69KK76 pKa = 11.0RR77 pKa = 11.84AAQRR81 pKa = 3.36

Molecular weight: 8.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

97

0

97

21298

36

1991

219.6

23.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.198 ± 0.346

1.038 ± 0.149

6.573 ± 0.253

5.94 ± 0.342

2.305 ± 0.1

9.658 ± 0.665

1.995 ± 0.169

4.587 ± 0.19

2.925 ± 0.178

7.94 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.089 ± 0.119

2.888 ± 0.214

6.043 ± 0.278

3.094 ± 0.131

6.752 ± 0.31

4.939 ± 0.227

7.123 ± 0.233

7.541 ± 0.196

1.855 ± 0.138

2.517 ± 0.105
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski