Lottia gigantea (Giant owl limpet)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Spiralia; Lophotrochozoa; Mollusca; Gastropoda; Patellogastropoda; Lottioidea; Lottiidae; Lottia

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 23675 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V3ZQS5|V3ZQS5_LOTGI Uncharacterized protein OS=Lottia gigantea OX=225164 GN=LOTGIDRAFT_235302 PE=3 SV=1
EEE2 pKa = 4.92DDD4 pKa = 3.65KK5 pKa = 11.27LLTRR9 pKa = 11.84VVIFEEE15 pKa = 4.49VSTLKKK21 pKa = 10.66KKK23 pKa = 10.92SIPDDD28 pKa = 3.63DD29 pKa = 3.59LLVLVQFVLLDDD41 pKa = 3.59GGSLCSSILSDDD53 pKa = 5.44DD54 pKa = 3.91VLDDD58 pKa = 3.86GGSLCSSILSDDD70 pKa = 5.44DD71 pKa = 3.91VLDDD75 pKa = 3.86GGSLCSSILSDDD87 pKa = 5.44DD88 pKa = 3.91VLDDD92 pKa = 3.86GGSLCSSILSDDD104 pKa = 5.44DD105 pKa = 3.91VLDDD109 pKa = 3.86GGSLCSSLLSDDD121 pKa = 5.46DD122 pKa = 4.36VLDDD126 pKa = 3.86GGSLCSSILSDDD138 pKa = 4.55DD139 pKa = 3.48GMTYYY144 pKa = 10.11SPPQSLLSYYY154 pKa = 10.48AAEEE158 pKa = 5.37MRR160 pKa = 11.84QHHH163 pKa = 6.88NDDD166 pKa = 4.66IEEE169 pKa = 4.89ISDDD173 pKa = 3.33HHH175 pKa = 6.19LTKKK179 pKa = 10.72K

Molecular weight: 18.95 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V3ZXA6|V3ZXA6_LOTGI DNA replication licensing factor MCM7 OS=Lottia gigantea OX=225164 GN=MCM7 PE=3 SV=1
RR1 pKa = 7.42QNQFLRR7 pKa = 11.84INTTLFLNQFLQINITLFLNQFLQINITLFLNQFLRR43 pKa = 11.84INITLFLNQFLRR55 pKa = 11.84INITLFLNQFLRR67 pKa = 11.84INITLFLNLFLRR79 pKa = 11.84INITLFLNQFLRR91 pKa = 11.84INITLFLNQFLQINITLFLNQFLQINITLFLNQFLQINITLFLNQFLRR139 pKa = 11.84INIILFLNQFLQINITLFLNQFLQINITLFLNQFLRR175 pKa = 11.84INIILFLNQFLRR187 pKa = 11.84INITLFLNQFLRR199 pKa = 11.84INITLFLNLFLRR211 pKa = 11.84INITLFLNLFLRR223 pKa = 11.84INITLFF229 pKa = 3.39

Molecular weight: 27.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

23671

4

23675

8983955

49

10830

379.5

42.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.981 ± 0.014

2.317 ± 0.019

5.856 ± 0.016

6.233 ± 0.021

4.205 ± 0.013

5.515 ± 0.02

2.418 ± 0.01

6.454 ± 0.017

6.993 ± 0.02

8.83 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.315 ± 0.008

5.454 ± 0.016

4.546 ± 0.016

4.069 ± 0.015

4.761 ± 0.017

8.019 ± 0.027

6.074 ± 0.019

6.2 ± 0.014

1.107 ± 0.007

3.649 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski