Zunongwangia atlantica 22II14-10F7

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Zunongwangia; Zunongwangia atlantica

Average proteome isoelectric point is 6.24

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y1T682|A0A1Y1T682_9FLAO DUF3817 domain-containing protein OS=Zunongwangia atlantica 22II14-10F7 OX=1185767 GN=IIF7_05872 PE=4 SV=1
MM1 pKa = 7.47KK2 pKa = 10.34KK3 pKa = 10.74SNFNQLFKK11 pKa = 10.84PVIFTLGILLFSINLEE27 pKa = 4.08AQCPIGNVTINSQSEE42 pKa = 4.37LIQFQNNYY50 pKa = 8.71PNCTIINANLSISSLVTDD68 pKa = 4.46LTPLQNIEE76 pKa = 4.45EE77 pKa = 4.36IKK79 pKa = 11.08GDD81 pKa = 3.7LTISNTNIEE90 pKa = 4.55DD91 pKa = 3.73LTTEE95 pKa = 4.47LSISKK100 pKa = 10.42INGDD104 pKa = 4.31LNIINNNLLRR114 pKa = 11.84HH115 pKa = 5.3VNSFNEE121 pKa = 4.06LTEE124 pKa = 3.89VSNINIDD131 pKa = 3.9RR132 pKa = 11.84ISGYY136 pKa = 10.43FDD138 pKa = 3.62GFNKK142 pKa = 10.32LEE144 pKa = 4.08KK145 pKa = 11.09VNTIDD150 pKa = 5.09LNNLGMSLDD159 pKa = 3.53AFQNLKK165 pKa = 10.35FVEE168 pKa = 4.56EE169 pKa = 4.14SLIMDD174 pKa = 4.85DD175 pKa = 5.07LGLALRR181 pKa = 11.84IYY183 pKa = 10.11QAFNKK188 pKa = 9.7VEE190 pKa = 4.18YY191 pKa = 10.02IKK193 pKa = 10.96GSLVIRR199 pKa = 11.84DD200 pKa = 3.89YY201 pKa = 11.33PHH203 pKa = 7.18GIGLFSEE210 pKa = 4.83LKK212 pKa = 10.94YY213 pKa = 10.59IGNDD217 pKa = 3.08LFINRR222 pKa = 11.84TGINEE227 pKa = 3.68IEE229 pKa = 4.38GFNKK233 pKa = 10.4LEE235 pKa = 4.67SIGSLGGGDD244 pKa = 4.07FEE246 pKa = 4.4ITEE249 pKa = 4.34SLEE252 pKa = 3.95LTVFTGFNNLQRR264 pKa = 11.84VYY266 pKa = 10.83GNMVITGNPEE276 pKa = 3.6LPEE279 pKa = 4.21ISGFYY284 pKa = 10.29NLEE287 pKa = 4.29KK288 pKa = 9.67IDD290 pKa = 3.83RR291 pKa = 11.84ALIIQNNNRR300 pKa = 11.84LAALTGLEE308 pKa = 3.93NLNAVSNNFGNLGLIVSNNQEE329 pKa = 4.16LTDD332 pKa = 4.42CSAICNLLANNRR344 pKa = 11.84VYY346 pKa = 11.47DD347 pKa = 4.45DD348 pKa = 4.78VEE350 pKa = 4.0ILNNPSEE357 pKa = 4.31CSTRR361 pKa = 11.84QEE363 pKa = 4.18IEE365 pKa = 4.6SNCLPDD371 pKa = 4.43FDD373 pKa = 5.87NDD375 pKa = 5.46GIPDD379 pKa = 5.66DD380 pKa = 5.49IDD382 pKa = 6.38LDD384 pKa = 4.39DD385 pKa = 6.24DD386 pKa = 4.26NDD388 pKa = 5.07GIPDD392 pKa = 4.22IIEE395 pKa = 4.54DD396 pKa = 3.79NGILDD401 pKa = 4.3RR402 pKa = 11.84DD403 pKa = 3.83SDD405 pKa = 4.26NDD407 pKa = 3.58GSPDD411 pKa = 4.42KK412 pKa = 10.95RR413 pKa = 11.84DD414 pKa = 3.51LDD416 pKa = 4.05SDD418 pKa = 3.78NDD420 pKa = 3.58GCYY423 pKa = 10.52DD424 pKa = 3.49VLEE427 pKa = 5.0AGFNDD432 pKa = 4.64DD433 pKa = 5.43DD434 pKa = 6.0DD435 pKa = 5.55NGTLGNSPDD444 pKa = 3.82TVDD447 pKa = 3.97EE448 pKa = 5.04DD449 pKa = 3.95GLIINEE455 pKa = 4.29TTGYY459 pKa = 6.34TTPLDD464 pKa = 3.75EE465 pKa = 5.69DD466 pKa = 3.69NNGIYY471 pKa = 10.42DD472 pKa = 3.71FQEE475 pKa = 4.22AFTLSAGNDD484 pKa = 3.27ASINLCQEE492 pKa = 3.72GNAIDD497 pKa = 3.97LFNYY501 pKa = 9.82LGKK504 pKa = 10.14DD505 pKa = 3.36AQRR508 pKa = 11.84GGTWSPNLASNSSVFDD524 pKa = 3.95PSLDD528 pKa = 3.58SEE530 pKa = 4.85GIYY533 pKa = 10.3AYY535 pKa = 9.05TVNNYY540 pKa = 9.96CISQTAIIEE549 pKa = 4.54INFEE553 pKa = 4.08PQPEE557 pKa = 4.37PGLDD561 pKa = 3.54TNISICQYY569 pKa = 9.87EE570 pKa = 4.2EE571 pKa = 3.67AFRR574 pKa = 11.84LVDD577 pKa = 3.79YY578 pKa = 11.6LEE580 pKa = 5.06GNPDD584 pKa = 3.56SNGYY588 pKa = 8.71WEE590 pKa = 4.68PSLPNGIFDD599 pKa = 3.86PQTNEE604 pKa = 3.56EE605 pKa = 4.1GTYY608 pKa = 9.52TYY610 pKa = 10.32HH611 pKa = 7.49ISNEE615 pKa = 3.89NCEE618 pKa = 4.25VLTSEE623 pKa = 5.42IIISFLDD630 pKa = 3.32RR631 pKa = 11.84TEE633 pKa = 4.42TISYY637 pKa = 9.36EE638 pKa = 3.93YY639 pKa = 10.04STALNNDD646 pKa = 2.42GSYY649 pKa = 11.31NIFFEE654 pKa = 4.49TANEE658 pKa = 4.01SGYY661 pKa = 9.03TFSLDD666 pKa = 4.89DD667 pKa = 4.18MTDD670 pKa = 2.88QSNGYY675 pKa = 9.21FYY677 pKa = 10.81NISPGIHH684 pKa = 5.65QIKK687 pKa = 8.0ITEE690 pKa = 4.08INGCGIVNEE699 pKa = 4.24QIALIGFPKK708 pKa = 10.31YY709 pKa = 10.6FSPNSDD715 pKa = 2.71GMNDD719 pKa = 2.9FWQPLGIIDD728 pKa = 5.29NEE730 pKa = 4.35VQTYY734 pKa = 9.97IYY736 pKa = 10.65DD737 pKa = 3.4RR738 pKa = 11.84YY739 pKa = 9.44GTLLRR744 pKa = 11.84KK745 pKa = 9.57LQGDD749 pKa = 3.93EE750 pKa = 4.41HH751 pKa = 6.95WDD753 pKa = 3.13GSYY756 pKa = 10.63NGKK759 pKa = 9.42PMPEE763 pKa = 3.5DD764 pKa = 4.69DD765 pKa = 4.11YY766 pKa = 11.47WFKK769 pKa = 11.54ALIGQEE775 pKa = 4.32SEE777 pKa = 4.4QKK779 pKa = 10.8GHH781 pKa = 6.76FSLIRR786 pKa = 4.08

Molecular weight: 87.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y1T3M7|A0A1Y1T3M7_9FLAO Uncharacterized protein OS=Zunongwangia atlantica 22II14-10F7 OX=1185767 GN=IIF7_12102 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 10.15RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84KK13 pKa = 8.29NKK15 pKa = 9.34HH16 pKa = 4.03GFRR19 pKa = 11.84EE20 pKa = 4.05RR21 pKa = 11.84MASVNGRR28 pKa = 11.84KK29 pKa = 9.21VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.09GRR39 pKa = 11.84KK40 pKa = 7.97KK41 pKa = 10.73LSVSSEE47 pKa = 3.76NRR49 pKa = 11.84HH50 pKa = 4.47KK51 pKa = 10.5HH52 pKa = 4.49

Molecular weight: 6.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4100

0

4100

1425727

29

2394

347.7

39.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.407 ± 0.039

0.649 ± 0.011

5.752 ± 0.031

7.393 ± 0.037

5.249 ± 0.028

6.377 ± 0.036

1.72 ± 0.02

7.716 ± 0.036

7.595 ± 0.054

9.194 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.182 ± 0.017

6.081 ± 0.041

3.453 ± 0.017

3.564 ± 0.022

3.625 ± 0.021

6.6 ± 0.034

5.262 ± 0.028

5.816 ± 0.031

1.147 ± 0.013

4.219 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski