Sewage-associated circular DNA virus-33

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 8.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0B4UGP2|A0A0B4UGP2_9VIRU Capsid protein OS=Sewage-associated circular DNA virus-33 OX=1592100 PE=4 SV=1
MM1 pKa = 7.72PSSNSIQARR10 pKa = 11.84YY11 pKa = 8.33WLLTINDD18 pKa = 3.68TDD20 pKa = 5.58DD21 pKa = 3.62NVSWSPPTAINTGPWTIVQWLRR43 pKa = 11.84GQKK46 pKa = 10.26EE47 pKa = 3.63IGTNTNRR54 pKa = 11.84EE55 pKa = 3.64HH56 pKa = 5.85WQLFVAFKK64 pKa = 10.74KK65 pKa = 10.33KK66 pKa = 9.89IRR68 pKa = 11.84LSPVKK73 pKa = 10.31QLFGGARR80 pKa = 11.84VHH82 pKa = 6.83AEE84 pKa = 3.86PSRR87 pKa = 11.84SEE89 pKa = 3.73AAEE92 pKa = 3.84EE93 pKa = 4.38YY94 pKa = 10.77VFKK97 pKa = 10.81EE98 pKa = 3.97DD99 pKa = 3.1TAIANTRR106 pKa = 11.84FEE108 pKa = 4.79LGAKK112 pKa = 9.62AFNRR116 pKa = 11.84ASKK119 pKa = 9.72TDD121 pKa = 2.96WALAKK126 pKa = 10.31KK127 pKa = 9.55CAKK130 pKa = 9.54EE131 pKa = 3.93GNIDD135 pKa = 3.75GVPDD139 pKa = 3.64DD140 pKa = 5.46VYY142 pKa = 10.99IKK144 pKa = 10.76YY145 pKa = 10.9YY146 pKa = 9.57NTLKK150 pKa = 10.25TIAKK154 pKa = 10.26DD155 pKa = 3.58NMKK158 pKa = 9.92PAEE161 pKa = 4.41NLDD164 pKa = 3.83AVCGIWYY171 pKa = 8.71YY172 pKa = 11.25GPPGVGKK179 pKa = 7.53SHH181 pKa = 7.43RR182 pKa = 11.84ARR184 pKa = 11.84SEE186 pKa = 3.86FPGAYY191 pKa = 9.17MKK193 pKa = 9.38MQNKK197 pKa = 7.48WWCGYY202 pKa = 7.87QNEE205 pKa = 4.9DD206 pKa = 4.76FVILDD211 pKa = 4.49DD212 pKa = 5.14FDD214 pKa = 4.91SKK216 pKa = 11.49QLGHH220 pKa = 6.75HH221 pKa = 6.18LKK223 pKa = 9.88IWADD227 pKa = 3.17KK228 pKa = 10.16YY229 pKa = 11.67AFIAEE234 pKa = 4.4TKK236 pKa = 10.17GYY238 pKa = 10.25AINIRR243 pKa = 11.84PKK245 pKa = 10.77KK246 pKa = 10.4FIITSNYY253 pKa = 10.2SIDD256 pKa = 3.95QIFCEE261 pKa = 4.93DD262 pKa = 3.47SVLANAIKK270 pKa = 10.33RR271 pKa = 11.84RR272 pKa = 11.84FEE274 pKa = 3.74VRR276 pKa = 11.84EE277 pKa = 3.57IPLRR281 pKa = 11.84LFF283 pKa = 3.66

Molecular weight: 32.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0B4UGP2|A0A0B4UGP2_9VIRU Capsid protein OS=Sewage-associated circular DNA virus-33 OX=1592100 PE=4 SV=1
MM1 pKa = 7.74ANRR4 pKa = 11.84AKK6 pKa = 10.29SVPRR10 pKa = 11.84TPMSLQRR17 pKa = 11.84GRR19 pKa = 11.84SRR21 pKa = 11.84SRR23 pKa = 11.84SMRR26 pKa = 11.84TVSMRR31 pKa = 11.84SATRR35 pKa = 11.84SASRR39 pKa = 11.84RR40 pKa = 11.84VLSRR44 pKa = 11.84IGSSVLGRR52 pKa = 11.84VNPYY56 pKa = 10.42VGTALSLAPYY66 pKa = 9.85ARR68 pKa = 11.84AGLRR72 pKa = 11.84TLRR75 pKa = 11.84SIRR78 pKa = 11.84KK79 pKa = 8.87YY80 pKa = 10.52SKK82 pKa = 10.37KK83 pKa = 9.89NYY85 pKa = 9.13KK86 pKa = 9.88RR87 pKa = 11.84GRR89 pKa = 11.84KK90 pKa = 8.98SGGRR94 pKa = 11.84KK95 pKa = 8.63GAAFSKK101 pKa = 10.61SAGFFGGTLKK111 pKa = 10.76DD112 pKa = 3.74SKK114 pKa = 10.58LAPYY118 pKa = 9.99LSKK121 pKa = 11.11GVVQQYY127 pKa = 10.56EE128 pKa = 4.11LGDD131 pKa = 3.87VVSEE135 pKa = 4.33ASRR138 pKa = 11.84QVVVVGHH145 pKa = 5.31STCPPSRR152 pKa = 11.84IIYY155 pKa = 10.05ACFGSLLKK163 pKa = 11.04LLFRR167 pKa = 11.84KK168 pKa = 10.19AGIKK172 pKa = 9.12IKK174 pKa = 10.41NWEE177 pKa = 3.99EE178 pKa = 4.2PILEE182 pKa = 4.43GANIPARR189 pKa = 11.84IAIRR193 pKa = 11.84YY194 pKa = 8.32KK195 pKa = 10.39EE196 pKa = 3.82RR197 pKa = 11.84DD198 pKa = 3.66GYY200 pKa = 11.51VVTTHH205 pKa = 6.69EE206 pKa = 4.42FPITTSLTMGQLVLNMVLWINGFSAINFPGQFLSIQYY243 pKa = 8.74YY244 pKa = 10.34HH245 pKa = 7.55DD246 pKa = 3.97VGTLGSSRR254 pKa = 11.84LIAYY258 pKa = 9.08DD259 pKa = 3.13IDD261 pKa = 3.92MTSTTVQIYY270 pKa = 10.02CRR272 pKa = 11.84SNMKK276 pKa = 9.52IQNRR280 pKa = 11.84TINTSGNDD288 pKa = 3.14QDD290 pKa = 5.11SDD292 pKa = 3.67VDD294 pKa = 3.89NVPLYY299 pKa = 10.8GKK301 pKa = 9.92HH302 pKa = 5.21FTVKK306 pKa = 10.77YY307 pKa = 9.67NGTVYY312 pKa = 10.6RR313 pKa = 11.84DD314 pKa = 3.64YY315 pKa = 10.68NTPAVSGTPQLYY327 pKa = 9.19TDD329 pKa = 4.51PEE331 pKa = 4.51FGSLNWSALPSDD343 pKa = 4.39TGTSLYY349 pKa = 10.86KK350 pKa = 10.2EE351 pKa = 4.45VPEE354 pKa = 4.1KK355 pKa = 11.05SQFIGVKK362 pKa = 9.81SAGPAHH368 pKa = 7.31LDD370 pKa = 3.23PGEE373 pKa = 4.23VKK375 pKa = 9.7TSKK378 pKa = 11.21LEE380 pKa = 4.03FSKK383 pKa = 11.11SLNFNKK389 pKa = 10.38LMMLFKK395 pKa = 10.9AKK397 pKa = 10.59APGGGGFPRR406 pKa = 11.84GVPFYY411 pKa = 10.67FGEE414 pKa = 4.13TRR416 pKa = 11.84FFCFEE421 pKa = 3.75KK422 pKa = 10.15MINAVAMTAEE432 pKa = 4.34NAFKK436 pKa = 10.59LAFEE440 pKa = 4.85IDD442 pKa = 3.77LTVGAICSTYY452 pKa = 10.68EE453 pKa = 3.62NHH455 pKa = 5.33QTAKK459 pKa = 9.84LTFQQTGQLL468 pKa = 3.67

Molecular weight: 51.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

751

283

468

375.5

42.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.856 ± 0.797

1.198 ± 0.129

4.261 ± 1.046

4.394 ± 0.755

5.06 ± 0.068

7.59 ± 1.16

1.465 ± 0.181

5.992 ± 1.067

7.457 ± 0.825

7.057 ± 0.841

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.13 ± 0.43

5.06 ± 0.567

4.66 ± 0.04

3.329 ± 0.089

6.258 ± 0.574

7.989 ± 1.611

6.258 ± 0.786

5.859 ± 0.758

1.731 ± 1.08

4.394 ± 0.092
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski