Lysinibacillus phage vB_LspM-01

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GQ61|A0A2D1GQ61_9CAUD Uncharacterized protein OS=Lysinibacillus phage vB_LspM-01 OX=2041389 PE=4 SV=1
MM1 pKa = 7.71SDD3 pKa = 3.69SIVSFEE9 pKa = 4.18FLDD12 pKa = 5.32DD13 pKa = 3.86NGQLIAYY20 pKa = 7.51EE21 pKa = 4.05DD22 pKa = 4.36VYY24 pKa = 11.4FEE26 pKa = 4.45IGDD29 pKa = 4.27AIIHH33 pKa = 6.36IEE35 pKa = 4.28NISDD39 pKa = 3.77YY40 pKa = 11.35LMQGYY45 pKa = 9.91GIDD48 pKa = 3.62TYY50 pKa = 10.63EE51 pKa = 4.38LSDD54 pKa = 3.87AEE56 pKa = 4.38VIEE59 pKa = 4.51LVEE62 pKa = 4.11KK63 pKa = 10.62EE64 pKa = 4.39YY65 pKa = 9.53GTKK68 pKa = 10.15HH69 pKa = 5.51IRR71 pKa = 11.84RR72 pKa = 11.84LL73 pKa = 3.44

Molecular weight: 8.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GQC1|A0A2D1GQC1_9CAUD ISL3 family transposase OS=Lysinibacillus phage vB_LspM-01 OX=2041389 PE=4 SV=1
MM1 pKa = 7.31TKK3 pKa = 10.25VLKK6 pKa = 10.84NDD8 pKa = 3.34VNSSKK13 pKa = 8.11WTATGNEE20 pKa = 4.4MIKK23 pKa = 10.67CPVPGCHH30 pKa = 7.01HH31 pKa = 7.14IGLMITKK38 pKa = 6.84VHH40 pKa = 6.26CRR42 pKa = 11.84MAHH45 pKa = 4.69GLEE48 pKa = 3.96RR49 pKa = 11.84TEE51 pKa = 3.96VEE53 pKa = 4.27KK54 pKa = 10.84KK55 pKa = 10.15YY56 pKa = 10.68GFPSRR61 pKa = 11.84VIQKK65 pKa = 10.5GLGDD69 pKa = 3.74KK70 pKa = 10.51RR71 pKa = 11.84SNIPTYY77 pKa = 10.92NRR79 pKa = 11.84LRR81 pKa = 11.84KK82 pKa = 9.35SDD84 pKa = 3.73YY85 pKa = 11.19

Molecular weight: 9.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

13709

51

870

163.2

18.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.127 ± 0.561

0.773 ± 0.133

5.901 ± 0.286

7.601 ± 0.418

3.983 ± 0.173

6.908 ± 0.548

1.619 ± 0.153

7.061 ± 0.322

7.462 ± 0.548

8.06 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.035 ± 0.22

5.828 ± 0.206

3.035 ± 0.222

3.822 ± 0.245

4.61 ± 0.231

5.515 ± 0.272

6.105 ± 0.268

7.017 ± 0.245

1.269 ± 0.106

4.267 ± 0.192
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski